Tyrosinase based biosensor for the electrochemical determination of sulfamethoxazole

Abstract A disposable amperometric biosensor has been developed for the determination of sulfamethoxazole (SMX). Tyrosinase (TYR) has been cross-linked to screen-printed carbon electrodes previously modified by gold nanoparticles. The oxidation current recorded at +500 mV vs Ag/AgCl SPE has been related to SMX concentration. The biosensor showed an acceptable inter and intra immobilization assay, with a RSD of 5.8% ( n  = 4) and 6.7% ( n  = 4), respectively, in the concentration range from 20 μM to 0.2 mM. The capability of detection was 22.6 ± 2.1 μM for a probability of false positive and negative of 0.05. Finally, the developed biosensors have been successfully applied to the determination of SMX in different water samples.

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