Endothelial Morphometry by Image Analysis of Corneas Organ Cultured at 31 ° C

METHODS. The endothelia of 505 of the 559 corneas consecutively stored at the eye bank were routinely analyzed with Sambacornea image-analysis software (ver. 1.2.10; Tribvn, Châtillon, France) on three large-field images of 75

[1]  D. Hodge,et al.  Initial endothelial cell density and chronic endothelial cell loss rate in corneal transplants with late endothelial failure. , 1999, Ophthalmology.

[2]  E. Godehardt,et al.  [Endothelial evaluation of corneal transplants by digital imaging]. , 1999, Klinische Monatsblätter für Augenheilkunde.

[3]  D. Glasser,et al.  Corneal endothelial morphology after anterior chamber lens implantation. , 1985, Archives of ophthalmology.

[4]  When Sperling,et al.  Organ culture: the method of choice for preservation of human donor corneas , 1997 .

[5]  P Gain,et al.  Is manual counting of corneal endothelial cell density in eye banks still acceptable? The French experience , 2003, The British journal of ophthalmology.

[6]  Gilles Thuret,et al.  Comparison of two semiautomated methods for evaluating endothelial cells of eye bank corneas. , 2007, Investigative ophthalmology & visual science.

[7]  M J Doughty,et al.  Morphometry of the corneal endothelium in glassblowers compared to non-glassblowers. , 2002, Journal of photochemistry and photobiology. B, Biology.

[8]  P. Gain,et al.  Standard microlithographic mosaics to assess endothelial cell counting methods by light microscopy in eye banks using organ culture. , 2006, Investigative ophthalmology & visual science.

[9]  M. Balls,et al.  Organ culture , 1975, Nature.

[10]  N Efron,et al.  Effects of long-term extended contact lens wear on the human cornea. , 1985, Investigative ophthalmology & visual science.

[11]  B. Delbosc,et al.  Morphology, Histology, and Ultrastructure of Human C31 Organ-Cultured Corneas , 1995, Cornea.

[12]  P. Gain,et al.  Automated tri-image analysis of stored corneal endothelium , 2002, The British journal of ophthalmology.

[13]  J. McLaren,et al.  Variations in human corneal endothelial cell morphology and permeability to fluorescein with age. , 1988, Experimental eye research.

[14]  Sanjay V. Patel,et al.  Corneal endothelium and postoperative outcomes 15 years after penetrating keratoplasty. , 2005, Transactions of the American Ophthalmological Society.

[15]  P. Gain,et al.  Assessment of the human corneal endothelium: in vivo Topcon SP2000P specular microscope versus ex vivo sambacornea eye bank analyser , 2007, British Journal of Ophthalmology.

[16]  M. Matsuda,et al.  Morphological change in the corneal endothelium due to ultraviolet radiation in welders. , 1984, The British journal of ophthalmology.

[17]  M J Doughty,et al.  Toward a quantitative analysis of corneal endothelial cell morphology: a review of techniques and their application. , 1989, Optometry and vision science : official publication of the American Academy of Optometry.

[18]  M. Doughty,et al.  A clinical assessment of the anterior eye in arc welders , 2005, Clinical & experimental optometry.

[19]  M. Boulton,et al.  Apoptosis in the endothelium of human corneas for transplantation. , 2000, Investigative ophthalmology & visual science.

[20]  R O Schultz,et al.  Corneal endothelial changes in type I and type II diabetes mellitus. , 1984, American journal of ophthalmology.

[21]  P. Gain,et al.  Controlled study of the influence of storage medium type on endothelial assessment during corneal organ culture , 2004, British Journal of Ophthalmology.

[22]  P. Gain,et al.  Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique , 2002, The British journal of ophthalmology.

[23]  D. Rootman,et al.  Endothelial Cell Analysis of Corneas from Donor Eyes with an Intraocular Lens: A Comparative Study , 1997, Cornea.

[24]  M. Doughty Prevalence of ‘non‐hexagonal’ cells in the corneal endothelium of young Caucasian adults, and their inter‐relationships , 1998, Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians.

[25]  J. Aquavella,et al.  Automated pattern analysis of corneal endothelium. , 1979, Ophthalmology.

[26]  L. Laroche,et al.  Donor organ cultured corneal tissue selection before penetrating keratoplasty , 1998, The British journal of ophthalmology.

[27]  J. McLaren,et al.  Estimation of corneal endothelial pump function in long-term contact lens wearers. , 1999, Investigative ophthalmology & visual science.

[28]  R. Beck,et al.  Baseline Donor Characteristics in the Cornea Donor Study , 2005, Cornea.

[29]  J. Strahan,et al.  Effect of a desensitizing dentifrice on dentinal hypersensitivity. , 1989, Endodontics & dental traumatology.

[30]  C. McGhee,et al.  The effects of corneal parameters on the assessment of endothelial cell density in the elderly eye , 2004, British Journal of Ophthalmology.

[31]  P. Gain,et al.  Reproducibility of endothelial assessment during corneal organ culture: comparison of a computer-assisted analyzer with manual methods. , 2007, Investigative ophthalmology & visual science.

[32]  J. Aquavella,et al.  Pseudophakic Bullous Keratopathy: Relationship to Preoperative Corneal Endothelial Status , 1984 .

[33]  M. Stur,et al.  Automated digital image analysis of organ culture preserved donor corneas. , 1993, Ophthalmic research.

[34]  M. Reim,et al.  Metabolic changes of the human donor cornea during organ-culture. , 1999, Acta ophthalmologica Scandinavica.

[35]  H F Edelhauser,et al.  Correlation of corneal endothelial pump site density, barrier function, and morphology in wound repair. , 1985, Investigative ophthalmology & visual science.

[36]  R Manabe,et al.  Quantitative analysis of endothelial mosaic pattern changes in anterior keratoconus. , 1984, American journal of ophthalmology.

[37]  W M Bourne,et al.  Corneal endothelium five years after transplantation. , 1994, American journal of ophthalmology.

[38]  P. Gain,et al.  Urgent Need for Normalization of Corneal Graft Quality Controls in French Eye Banks , 2004, Transplantation.

[39]  J V Aquavella,et al.  Endothelial cell morphology and corneal deturgescence. , 1979, Annals of ophthalmology.

[40]  M. Baudrimont,et al.  Evaluation of the Deswelling Period in Dextran‐Containing Medium After Corneal Organ Culture , 1997, Cornea.

[41]  R O Schultz,et al.  Changes in the normal corneal endothelial cellular pattern as a function of age. , 1985, Current eye research.

[42]  J V Aquavella,et al.  The functional reserve of corneal endothelium. , 1978, Ophthalmology.

[43]  A. Graham,et al.  Morphometry of Cells and Guttae in Subjects With Normal or Guttate Endothelium With a Contour Detection Algorithm , 2005, Eye & contact lens.

[44]  C G Connor,et al.  Contact Lens‐Induced Corneal Endothelial Polymegathism: Functional Significance and Possible Mechanisms , 1986, American journal of optometry and physiological optics.

[45]  A. Ruggeri,et al.  A new system for the automatic estimation of endothelial cell density in donor corneas , 2005, British Journal of Ophthalmology.

[46]  W. Gillies,et al.  A comparison of corneal endothelial morphology in cornea guttata, Fuchs' dystrophy and bullous keratopathy. , 1988, Australian and New Zealand journal of ophthalmology.

[47]  G. M. Zoega,et al.  Prevalence and risk factors for cornea guttata in the Reykjavik Eye Study. , 2006, Ophthalmology.

[48]  W. M. Bourne,et al.  Long-term morphologic changes in the endothelium of transplanted corneas. , 1985, Archives of ophthalmology.

[49]  J. Aquavella,et al.  Cell size-shape relationships in corneal endothelium. , 1982, Investigative ophthalmology & visual science.

[50]  M. O'Neill,et al.  Benefits of Specular Microscopy in Evaluating Eye Donors Aged 66 and Older , 1995, Cornea.