Ribonucleoprotein particles from Escherichia coli

In exponential cultures, 25% of the dry weight of E. coli is accounted for by RNA. Of this about 80 to 90% is present in ribonucleoprotein particles and 10 to 20% in the “soluble” or “non-sedimentable” fraction. Magnesium stabilizes the particles, and on varying its concentration, four kinds of components are observed, with sedimentation constants of 30, 50, 70 and 100 S . 70 S is formed of one 30 S and one 50 S ; 100 S , of two 70 S . Each type of particle has been isolated. They all contain about 63% RNA and 37% protein, and have the same density. The molecular weights of the 30 S , 50 S and 70 S particles are about 0·7 × 10 6 , 1·8 × 10 6 , and 2·6 × 10 6 respectively.

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