Host specificity of DNA produced by Escherichia coli: XIII. Breakdown of cellular DNA upon growth in Ethionine of strains with r15+, rP1+ or rN3+ restriction phenotypes☆☆☆

Abstract All methionine-requiring strains of Escherichia coli which have been tested show a two-fold increase in DNA content when grown in the presence of ethionine or norleucine. In addition, E. coli strains with the restriction phenotype r 15 + , r P1 + or r N3 + will degrade their intracellular DNA when these methionine analogs are substituted for required methionine in the growth medium. Degradation does not occur in restriction-deficient mutants and in strains carrying the r K + , r A + or r B + phenotypes alone. Breakdown of DNA appears to be a characteristic of the restriction gene products rather than of the location of these genes on plasmid or chromosomal DNA molecules.

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