Frequent blood donations alter susceptibility of red blood cells to storage‐ and stress‐induced hemolysis

Frequent whole blood donations increase the prevalence of iron depletion in blood donors, which may subsequently interfere with normal erythropoiesis. The purpose of this study was to evaluate the associations between donation frequency and red blood cell (RBC) storage stability in a racially/ethnically diverse population of blood donors.

[1]  J. Widness,et al.  Red blood cell (RBC) survival determined in humans using RBCs labeled at multiple biotin densities , 2011, Transfusion.

[2]  A. Forster,et al.  Clinical effects of blood donor characteristics in transfusion recipients: protocol of a framework to study the blood donor–recipient continuum , 2015, BMJ Open.

[3]  J. Hayman,et al.  Frequent whole blood donors: understanding this population and predictors of lapse , 2017, Transfusion.

[4]  M. Hall.  National Heart, Lung, and Blood Institute , 2020, The Grants Register 2022.

[5]  Grier P Page,et al.  Ethnicity, sex, and age are determinants of red blood cell storage and stress hemolysis: results of the REDS-III RBC-Omics study. , 2017, Blood advances.

[6]  A. Tai,et al.  Inhibition of free radical-induced erythrocyte hemolysis by 2-O-substituted ascorbic acid derivatives. , 2007, Free radical biology & medicine.

[7]  Holger Kiesewetter,et al.  Daily doses of 20 mg of elemental iron compensate for iron loss in regular blood donors: a randomized, double‐blind, placebo‐controlled study , 2004, Transfusion.

[8]  H. Ullum,et al.  Association of Donor Age and Sex With Survival of Patients Receiving Transfusions , 2017, JAMA internal medicine.

[9]  M. Gladwin,et al.  Testosterone‐dependent sex differences in red blood cell hemolysis in storage, stress, and disease , 2016, Transfusion.

[10]  S. Lewis,et al.  Recommendations for reference method for haemoglobinometry in human blood (ICSH standard 1995) and specifications for international haemiglobinocyanide standard (4th edition). , 1996, Journal of clinical pathology.

[11]  H. Howie,et al.  Metabolic pathways that correlate with post-transfusion circulation of stored murine red blood cells , 2016, Haematologica.

[12]  M. Gladwin,et al.  Assessing the influence of component processing and donor characteristics on quality of red cell concentrates using quality control data , 2016, Vox sanguinis.

[13]  G. Brittenham,et al.  Iron-deficient erythropoiesis in blood donors and red blood cell recovery after transfusion: initial studies with a mouse model. , 2017, Blood transfusion = Trasfusione del sangue.

[14]  D. Wright,et al.  Quantification of body iron and iron absorption in the REDS‐II Donor Iron Status Evaluation (RISE) study , 2017, Transfusion.

[15]  D. Wright,et al.  Estimates of total body iron indicate 19 mg and 38 mg oral iron are equivalent for the mitigation of iron deficiency in individuals experiencing repeated phlebotomy , 2017, American journal of hematology.

[16]  J. A. Hopkins,et al.  Iron replacement therapy in the routine management of blood donors , 2012, Transfusion.

[17]  G. Bartosz,et al.  ‘PINK TEST’ AND OSMOTIC FRAGILITY TEST FOR THE DIAGNOSIS OF HEREDITARY SPHEROCYTOSIS: ANOTHER VIEW , 1989, European journal of haematology.

[18]  H. Klein,et al.  Teenage Blood Donors: Are We Asking Too Little and Taking Too Much? , 2017, Pediatrics.

[19]  Hadley Wickham,et al.  ggplot2 - Elegant Graphics for Data Analysis (2nd Edition) , 2017 .

[20]  S. Glynn,et al.  The National Heart, Lung, and Blood Institute Recipient Epidemiology and Donor Evaluation Study (REDS‐III): a research program striving to improve blood donor and transfusion recipient outcomes , 2014, Transfusion.

[21]  D. Bellissimo,et al.  Behavioral, biochemical, and genetic analysis of iron metabolism in high‐intensity blood donors , 2008, Transfusion.

[22]  I. Papassideri,et al.  Donor variation effect on red blood cell storage lesion: a multivariable, yet consistent, story , 2016, Transfusion.

[23]  S. Glynn,et al.  Oral iron supplementation after blood donation: a randomized clinical trial. , 2015, JAMA.

[24]  Melissa S. Murphy.  The epidemiology of transfusion: where blood goes and why we should care about it , 2017, Transfusion.

[25]  S. Glynn,et al.  Effect of iron supplementation on iron stores and total body iron after whole blood donation , 2016, Transfusion.

[26]  O. Hermine,et al.  The real superdonors , 2014, Transfusion.

[27]  Brigden Ml.  Clinical utility of serum tests for iron deficiency in hospitalized patients. , 1990 .

[28]  H. Ullum,et al.  Predictors of iron levels in 14,737 Danish blood donors: results from the Danish Blood Donor Study , 2013, Transfusion.

[29]  A. Forster,et al.  Association of Blood Donor Age and Sex With Recipient Survival After Red Blood Cell Transfusion. , 2016, JAMA internal medicine.

[30]  E. Murphy,et al.  Demographic and epidemiologic characterization of transfusion recipients from four US regions: evidence from the REDS‐III recipient database , 2017, Transfusion.

[31]  M. Scott,et al.  Clinical utility of the soluble transferrin receptor and comparison with serum ferritin in several populations. , 1998, Clinical chemistry.

[32]  A. Mast,et al.  The strategies to reduce iron deficiency in blood donors randomized trial: design, enrolment and early retention , 2015, Vox sanguinis.

[33]  M. Germain,et al.  Trends in US minority red blood cell unit donations , 2017, Transfusion.

[34]  A. Mast,et al.  A randomized, blinded, placebo‐controlled trial of education and iron supplementation for mitigation of iron deficiency in regular blood donors , 2016, Transfusion.

[35]  Janet S. Lee,et al.  Sickle Cell Trait Increases Red Blood Cell Storage Hemolysis and Post-Transfusion Clearance in Mice , 2016, EBioMedicine.

[36]  A. Forster,et al.  Effect of Blood Donor Characteristics on Transfusion Outcomes: A Systematic Review and Meta-Analysis. , 2016, Transfusion medicine reviews.

[37]  J. Lagerberg,et al.  A method for red blood cell biotinylation in a closed system , 2018, Transfusion.

[38]  J. Kiss.  Laboratory and genetic assessment of iron deficiency in blood donors. , 2015, Clinics in laboratory medicine.

[39]  A. Mast,et al.  The operational implications of donor behaviors following enrollment in STRIDE (Strategies to Reduce Iron Deficiency in blood donors) , 2017, Transfusion.

[40]  S. Glynn,et al.  Iron deficiency in blood donors: the REDS‐II Donor Iron Status Evaluation (RISE) study , 2012, Transfusion.

[41]  M. Gladwin,et al.  Nitric oxide, hemolysis, and the red blood cell storage lesion: interactions between transfusion, donor, and recipient , 2012, Transfusion.

[42]  Melanie C. Chansky,et al.  Qualitative assessment of pica experienced by frequent blood donors , 2017, Transfusion.

[43]  Marion C Lanteri,et al.  Intradonor reproducibility and changes in hemolytic variables during red blood cell storage: results of recall phase of the REDS‐III RBC‐Omics study , 2018, Transfusion.

[44]  Grier P Page,et al.  Blood, sweat, and tears: Red Blood Cell‐Omics study objectives, design, and recruitment activities , 2018, Transfusion.