Pathogenesis of the human opportunistic pathogen Pseudomonas aeruginosa PA14 in Arabidopsis.

The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 is a multihost pathogen that can infect Arabidopsis. We found that PA14 pathogenesis in Arabidopsis involves the following steps: attachment to the leaf surface, congregation of bacteria at and invasion through stomata or wounds, colonization of intercellular spaces, and concomitant disruption of plant cell wall and membrane structures, basipetal movement along the vascular parenchyma, and maceration and rotting of the petiole and central bud. Distinctive features of P. aeruginosa pathogenesis are that the surface of mesophyll cell walls adopt an unusual convoluted or undulated appearance, that PA14 cells orient themselves perpendicularly to the outer surface of mesophyll cell walls, and that PA14 cells make circular perforations, approximately equal to the diameter of P. aeruginosa, in mesophyll cell walls. Taken together, our data show that P. aeruginosa strain PA14 is a facultative pathogen of Arabidopsis that is capable of causing local and systemic infection, which can result in the death of the infected plant.

[1]  F. Ausubel,et al.  Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[2]  A. C. Braun,et al.  Pseudomonas aeruginosa: Its Rôle as a Plant Pathogen , 1942, Journal of bacteriology.

[3]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[4]  B. Postic,et al.  Introduction of Pseudomonas aeruginosa into a Hospital via Vegetables , 1972, Applied microbiology.

[5]  C. Boucher,et al.  Ralstonia solanacearum produces Hrp‐dependent pili that are required for PopA secretion but not for attachment of bacteria to plant cells , 2000, Molecular microbiology.

[6]  F. Ausubel,et al.  Isolation of new Arabidopsis mutants with enhanced disease susceptibility to Pseudomonas syringae by direct screening. , 1998, Genetics.

[7]  C. Boucher,et al.  The hrpB and hrpG regulatory genes of Ralstonia solanacearum are required for different stages of the tomato root infection process. , 2000, Molecular plant-microbe interactions : MPMI.

[8]  F. Ausubel,et al.  Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[9]  F. Ausubel,et al.  Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Frederick M. Ausubel,et al.  Molecular Mechanisms of Bacterial Virulence Elucidated Using a Pseudomonas Aeruginosa– Caenorhabditis Elegans Pathogenesis Model , 2022 .

[11]  Xinnian Dong,et al.  Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance. , 1994, The Plant cell.

[12]  W. H. Burkholder.  Sour skin, a bacterial rot of Onion bulbs. , 1950 .

[13]  G. Pier,et al.  Pseudomonas aeruginosa invasion of and multiplication within corneal epithelial cells in vitro , 1995, Infection and immunity.

[14]  J. Goldberg,et al.  Role of Mutant CFTR in Hypersusceptibility of Cystic Fibrosis Patients to Lung Infections , 1996, Science.

[15]  M. Romantschuk.  Attachment of plant pathogenic bacteria to plant surfaces. , 1992, Annual review of phytopathology.

[16]  J. Cho.  Ornamental Plants as Carriers of Pseudomonas aeruginosa , 1975 .

[17]  F. Ausubel,et al.  Positive Correlation between Virulence ofPseudomonas aeruginosa Mutants in Mice and Insects , 2000, Journal of bacteriology.

[18]  C. D. Cox,et al.  Augmentation of oxidant injury to human pulmonary epithelial cells by the Pseudomonas aeruginosa siderophore pyochelin , 1997, Infection and immunity.

[19]  B. Postic,et al.  Introduction of Pseudomonas aeruginosa into a Hospital via Vegetables , 1972, Applied microbiology.

[20]  J. Lyczak,et al.  Cystic Fibrosis Transmembrane Conductance Regulator-Mediated Corneal Epithelial Cell Ingestion of Pseudomonas aeruginosaIs a Key Component in the Pathogenesis of Experimental Murine Keratitis , 1999, Infection and Immunity.

[21]  F. Ausubel,et al.  Correlation of defense gene induction defects with powdery mildew susceptibility in Arabidopsis enhanced disease susceptibility mutants. , 1998, The Plant journal : for cell and molecular biology.

[22]  R. Kolter,et al.  Green fluorescent protein as a marker for Pseudomonas spp , 1997, Applied and environmental microbiology.

[23]  F. Ausubel,et al.  Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene. , 1991, The Plant cell.

[24]  F. Ausubel,et al.  Common virulence factors for bacterial pathogenicity in plants and animals. , 1995, Science.