Molecular identification of sex in the monomorphic breed of pigeons

In many avian species, especially in monomorphic species and breeds, sex identification creates a serious problem, as they do not show any phenotypic differences. One of such breeds is the Wroclaw Meat Pigeon. In this study, molecular identification of sex with P2 and P8 primers used for the CHD1 (chromo-helicase-DNA-binding-protein) gene amplification was performed. Peripheral blood samples were analyzed from 46 birds, and their DNA was isolated with the phenol-chloroform method. The fragments (370 bp CHD1-Z; 350 bp CHD1-W) obtained from the PCR were cut with the BsuRI. Only the sequence in the Z chromosome was cut into fragments of 305 and 65 bp by the restriction enzyme. The difference between CHD1-Z and CHD1-W was visualized in 3% agarose gel. A single band was identified as male, whereas two bands (plus 1 invisible) were identified as female. Consequently, 23 specimens in each sex were identified.

[1]  Jasdeep Singh,et al.  Z-chromosome linked dinucleotide strs: association with reproduction traits in chicken , 2014 .

[2]  Jakaria,et al.  Avian Sex Determination Based on Chromo Helicase DNA-binding (CHD) Genes Using Polymerase Chain Reaction (PCR) , 2013 .

[3]  J. Stevanović,et al.  Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing. , 2013, Zoo biology.

[4]  S. Giokas,et al.  Efficiency and Accuracy of PCR-Based Sex Determination Methods in the European Phalacrocoracidae , 2013 .

[5]  H. Malik,et al.  Phylogenetic analysis of ratite bird emu (Dromaius Novaehollandiae) using sex specific sequences. , 2013 .

[6]  J. A. Cabral,et al.  Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR)-based methods. , 2012, Theriogenology.

[7]  I. Volodin,et al.  THE TECHNIQUE OF NONINVASIVE DISTANT SEXING FOR FOUR MONOMORPHIC DENDROCYGNA WHISTLING DUCK SPECIES BY THEIR LOUD WHISTLES , 2009 .

[8]  A. Sinclair,et al.  Avian sex determination: what, when and where? , 2007, Cytogenetic and Genome Research.

[9]  H. Cerit,et al.  Sex Determination by CHDW and CHDZ Genes of Avian Sex Chromosomes in Nymphicus hollandicus , 2007 .

[10]  Chean-Ping Wu,et al.  A novel sex-specific DNA marker in Columbidae birds. , 2007, Theriogenology.

[11]  A. Dubiec,et al.  Molecular techniques for sex identification in birds , 2006 .

[12]  D. Soglia,et al.  A non-invasive test for sex identification in short-toed Eagle (Circaetus gallicus). , 2004, Molecular and cellular probes.

[13]  H. Richner.  AVIAN LAPAROSCOPY AS A FIELD TECHNIQUE FOR SEXING BIRDS AND AN ASSESSMENT OF ITS EFFECTS ON WILD BIRDS , 2004 .

[14]  F. Pernasetti,et al.  Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers , 2003 .

[15]  A. Guzmán-Velasco,et al.  Loss of restriction site DdeI, used for avian molecular sexing, in Oreophasis derbianus. , 2002, Reproduction in domestic animals = Zuchthygiene.

[16]  H. Ellegren,et al.  A simple and universal method for molecular sexing of non-ratite birds , 1999 .

[17]  N. W. Kahn,et al.  Chromosome-Specific Intron Size Differences in the Avian CHD Gene Provide an Efficient Method for Sex Identification in Birds , 1998 .

[18]  R. Griffiths,et al.  A DNA test to sex most birds , 1998, Molecular ecology.

[19]  S. Daan,et al.  Sex identification in birds using two CHD genes , 1996, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[20]  Richard Griffiths,et al.  Sex of the last wild Spix's macaw , 1995, Nature.

[21]  R. Griffiths,et al.  The isolation of molecular genetic markers for the identification of sex. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[22]  J. Longmire,et al.  Gender identification in birds using microsatellite DNA fingerprint analysis , 1993 .