Decreased semen volume and spermatozoa motility in HIV-1-infected patients under antiretroviral treatment.

Inconsistent results have been reported for the semen quality in HIV-infected men, due to the biases inherent in some studies. The objective of the present study was to investigate the semen parameters in HIV-1-infected patients and to compare their sperm characteristics with those of a control group of fertile, noninfected men. Factors implicated in semen alterations in HIV-1 patients were also analyzed. HIV-infected men (n=190), of whom 91% were undergoing antiretroviral therapy, and 218 fertile men were studied. Infertility risk factors were recorded and clinical examinations were performed for both groups. Records of history of HIV infection, antiretroviral treatment, and HIV-1 RNA detection in the blood as well as HIV-1 genome detection in the semen were obtained for the infected patients. Semen volumes, percentages of progressive motile spermatozoa, total sperm counts, and polymorphonuclear cell counts were decreased, while the pH values and spermatozoa multiple anomaly indices were increased in HIV-infected patients. Even after adjustment for possible sources of bias, the decreases in semen volume and progressive motility and the increase in pH remained significant. The present study demonstrates sperm motility and ejaculate volume alterations in HIV-1-infected patients, most of whom were receiving antiretroviral therapy. In HIV-1 patients, further longitudinal studies are required to analyze the impact of treatment regimen on sperm parameter alterations.

[1]  Adeeba Kamarulzaman,et al.  AIDS Res Hum Retroviruses , 2006 .

[2]  K. Tashima,et al.  Assisted Reproduction in HIV-Discordant Couples , 2006 .

[3]  A. Collier,et al.  Lower Genitourinary Tract Sources of Seminal HIV , 2006, Journal of acquired immune deficiency syndromes.

[4]  M. Hardy,et al.  Clinical Male Infertility and Assisted Reproduction , 2006 .

[5]  V. de Leo,et al.  ICSI for treatment of human immunodeficiency virus and hepatitis C virus-serodiscordant couples with infected male partner. , 2005, Human reproduction.

[6]  M. Meseguer,et al.  Semen characteristics in human immunodeficiency virus (HIV)- and hepatitis C (HCV)-seropositive males: predictors of the success of viral removal after sperm washing. , 2005, Human reproduction.

[7]  S. Repping,et al.  Semen parameters of a semen donor before and after infection with human immunodeficiency virus type 1: case report. , 2004, Human reproduction.

[8]  J. Nicopoullos,et al.  The effect of human immunodeficiency virus on sperm parameters and the outcome of intrauterine insemination following sperm washing. , 2004, Human reproduction.

[9]  C. Pasquier,et al.  Insemination with isolated and virologically tested spermatozoa is a safe way for human immunodeficiency type 1 virus-serodiscordant couples with an infected male partner to have a child. , 2004, Fertility and sterility.

[10]  P. Reiss,et al.  Is the male genital tract really a sanctuary site for HIV? Arguments that it is not. , 2004, AIDS.

[11]  A. Berrebi,et al.  Factors of intermittent HIV-1 excretion in semen and efficiency of sperm processing in obtaining spermatozoa without HIV-1 genomes , 2004, AIDS.

[12]  Roland Tubiana,et al.  Evidence of genotypic resistance diversity of archived and circulating viral strains in blood and semen of pre-treated HIV-infected men , 2004, AIDS.

[13]  B. Jégou,et al.  Viruses in semen and male genital tissues--consequences for the reproductive system and therapeutic perspectives. , 2004, Current pharmaceutical design.

[14]  M. Meseguer,et al.  ASSISTED REPRODUCTION IN HIV AND HCV INFECTED MEN OF SERODISCORDANT COUPLES , 2004, Archives of andrology.

[15]  C. Rongières,et al.  Assisted reproduction techniques for HIV serodiscordant couples: 18 months of experience. , 2003, Human reproduction.

[16]  M. Sauer,et al.  Providing assisted reproductive care to male haemophiliacs infected with human immunodeficiency virus: preliminary experience , 2003, Haemophilia : the official journal of the World Federation of Hemophilia.

[17]  T. Toth,et al.  Seasonal variation and age-related changes in human semen parameters. , 2003, Journal of andrology.

[18]  P. Reynier,et al.  Increased sperm mitochondrial DNA content in male infertility. , 2003, Human reproduction.

[19]  P. Vernazza,et al.  Mitochondrial DNA and sperm quality in patients under antiretroviral therapy. , 2002, AIDS.

[20]  C. Rouzioux,et al.  Semen alterations in HIV-1 infected men. , 2002, Human reproduction.

[21]  M. Sauer,et al.  Successive pregnancies with delivery of two healthy infants in a couple who was discordant for human immunodeficiency virus infection. , 2002, Fertility and sterility.

[22]  M. Sauer,et al.  Establishing a clinical program for human immunodeficiency virus 1-seropositive men to father seronegative children by means of in vitro fertilization with intracytoplasmic sperm injection. , 2002, American journal of obstetrics and gynecology.

[23]  M. Poirier,et al.  Transplacental genotoxicity of combined antiretroviral nucleoside analogue therapy in Erythrocebus patas monkeys. , 2002 .

[24]  D. Pillay,et al.  Antiretroviral drug concentrations in semen of HIV-infected men: differential penetration of indinavir, ritonavir and saquinavir. , 2001, The Journal of antimicrobial chemotherapy.

[25]  D. Dunson,et al.  Antiretroviral therapy effects on genetic and morphologic end points in lymphocytes and sperm of men with human immunodeficiency virus infection. , 2001, The Journal of infectious diseases.

[26]  B. Jégou,et al.  Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System , 2001, Microbiology and Molecular Biology Reviews.

[27]  S. Taylor,et al.  Sperm mitochondrial DNA deletions as a consequence of long term highly active antiretroviral therapy. , 2001, AIDS.

[28]  M. Poirier,et al.  Plasma Drug Levels Compared with DNA Incorporation of 3′-azido-3′-Deoxythymidine (AZT) in Adult Cynomolgus (Macaca fascicularis) Monkeys , 2001, Experimental biology and medicine.

[29]  C. Chougnet,et al.  Incorporation of Zidovudine into Cord Blood DNA of Infants and Peripheral Blood DNA of Their HIV‐1‐Positive Mothers , 2000, Annals of the New York Academy of Sciences.

[30]  C. Gilling‐Smith,et al.  HIV prevention. Assisted reproduction in HIV-discordant couples. , 2000, The AIDS reader.

[31]  F. Comhaire.  Clinical andrology: from evidence-base to ethics. The 'E' quintet in clinical andrology. , 2000, Human reproduction.

[32]  A. Berrebi,et al.  Sperm washing and virus nucleic acid detection to reduce HIV and hepatitis C virus transmission in serodiscordant couples wishing to have children , 2000, AIDS.

[33]  N. Mcclure,et al.  Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa. , 2000, Human reproduction.

[34]  T. Quinn,et al.  Viral load and heterosexual transmission of human immunodeficiency virus type 1. Rakai Project Study Group. , 2000, The New England journal of medicine.

[35]  R. Tarone,et al.  Incorporation of zidovudine into leukocyte DNA from HIV-1-positive adults and pregnant women, and cord blood from infants exposed in utero. , 1999, AIDS.

[36]  C. Rouzioux,et al.  Detection and quantification of HIV-1 in semen: identification of a subpopulation of men at high potential risk of viral sexual transmission. , 1999, AIDS.

[37]  J. Krieger,et al.  Effects of clinical stage and immunological status on semen analysis results in human immunodeficiency virus type 1‐seropositive men , 1998, Andrologia.

[38]  R. Byrn,et al.  Analysis of human immunodeficiency virus in semen: indications of a genetically distinct virus reservoir. , 1998, Journal of reproductive immunology.

[39]  P. Vernazza,et al.  Resistance of HIV‐1 to antiretroviral agents in blood and seminal plasma: implications for transmission , 1998, AIDS.

[40]  J. Smeitink,et al.  Adverse effects of reverse transcriptase inhibitors: mitochondrial toxicity as common pathway , 1998, AIDS.

[41]  E. Ruiz-Pesini,et al.  Correlation of sperm motility with mitochondrial enzymatic activities. , 1998, Clinical chemistry.

[42]  R. Expósito,et al.  Human immunodeficiency virus type 1--serodiscordant couples can bear healthy children after undergoing intrauterine insemination. , 1998, Fertility and sterility.

[43]  L. Fitzgerald,et al.  Human immunodeficiency virus in semen arises from a genetically distinct virus reservoir. , 1998, AIDS research and human retroviruses.

[44]  A. Collier,et al.  Association between culturable human immunodeficiency virus type 1 (HIV-1) in semen and HIV-1 RNA levels in semen and blood: evidence for compartmentalization of HIV-1 between semen and blood. , 1998, The Journal of infectious diseases.

[45]  L. Kingsley,et al.  High viral load in semen of human immunodeficiency virus type 1-infected men at all stages of disease and its reduction by therapy with protease and nonnucleoside reverse transcriptase inhibitors , 1997, Journal of virology.

[46]  D. Pillay,et al.  Semen characteristics in HIV-1 positive men and the effect of semen washing. , 1997, Genitourinary medicine.

[47]  P. Vernazza,et al.  Quantification of HIV in semen: correlation with antiviral treatment and immune status , 1997, AIDS.

[48]  R. Royce,et al.  Sexual transmission of HIV. , 1997, The New England journal of medicine.

[49]  T. O'Brien,et al.  Correlation of HIV-1 RNA levels in plasma and heterosexual transmission of HIV-1 from infected transfusion recipients. , 1996, Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association.

[50]  F. Dondero,et al.  Semen analysis in HIV seropositive men and in subjects at high risk for HIV infection. , 1996, Human reproduction.

[51]  D. Anderson,et al.  Heterosexual HIV transmission. , 1995, Infectious agents and disease.

[52]  D. Johns.  Seminars in medicine of the Beth Israel Hospital, Boston. Mitochondrial DNA and disease. , 1995, The New England journal of medicine.

[53]  M. Dalakas,et al.  Mitochondrial toxicity of antiviral drugs , 1995, Nature Medicine.

[54]  J. Auger,et al.  Debate about sperm count decline. , 1998, Environmental health perspectives.

[55]  P. Vernazza,et al.  Detection and biologic characterization of infectious HIV-1 in semen of seropositive men , 1994, AIDS.

[56]  D. Anderson,et al.  The effects of disease progression and zidovudine therapy on semen quality in human immunodeficiency virus type 1 seropositive men. , 1994, Fertility and sterility.

[57]  P. Øian,et al.  Andrology: Mitochondrial disease and reduced sperm motility , 1993 .

[58]  A. Semprini.  Insemination of HIV-negative women with processed semen of HIV-positive partners , 1993, The Lancet.

[59]  G. Pardi,et al.  Insemination of HIV-negative women with processed semen of HIV-positive partners , 1992, The Lancet.

[60]  David Handelsman,et al.  Semen analysis in human immunodeficiency virus infection. , 1992 .

[61]  T. O'Brien,et al.  Effects of disease stage and zidovudine therapy on the detection of human immunodeficiency virus type 1 in semen. , 1992, JAMA.

[62]  A. Collier,et al.  Fertility parameters in men infected with human immunodeficiency virus. , 1991, The Journal of infectious diseases.

[63]  M. Plantavid,et al.  Increased levels of serum follicle-stimulating hormone and luteinizing hormone associated with intrinsic testicular hyperthermia in oligospermic infertile men. , 1989, The Journal of clinical endocrinology and metabolism.

[64]  P. Jouannet,et al.  Male factors and the likelihood of pregnancy in infertile couples. I. Study of sperm characteristics. , 1988, International journal of andrology.

[65]  H. Balfour,et al.  Concurrent zidovudine levels in semen and serum determined by radioimmunoassay in patients with AIDS or AIDS-related complex. , 1988, JAMA.

[66]  D. Loutradis,et al.  Birth of two infants who were seronegative for human immunodeficiency virus type 1 (HIV-1) after intracytoplasmic injection of sperm from HIV-1-seropositive men. , 2001, Fertility and sterility.