The expression of E-cadherin and catenins in sporadic colorectal carcinoma.

The E-cadherin/catenin complex plays a major role in epithelial cell-cell adhesion. Immunohistochemical studies have highlighted perturbation in the expression and distribution of E-cadherin and catenins in sporadic colorectal neoplasms. In this study, we compared the expression of E-cadherin and catenins (alpha-, beta-, and gamma-catenin) in 30 sporadic colorectal carcinomas with that in the adjacent nonneoplastic mucosa and assessed whether any perturbation in the level of expression occurred at the messenger RNA (mRNA) or protein level. We also compared the expression of E-cadherin and catenins in 13 lymph node deposits and the primary tumors. Immunohistochemistry was used to study the level of expression and cellular distribution of E-cadherin and catenins. Levels of mRNA were studied by in situ hybridization. E-cadherin and catenin immunoreactivity was increased with cytoplasmic accumulation in more than 85% of the neoplasms. There were marked increases in the levels of mRNA in the carcinomas compared with the nonneoplastic mucosa. Nuclear localization of beta-catenin was higher at the invasive margin of some tumors, but expression of E-cadherin and catenin transcripts in the lymph node deposits showed no consistent relationship to that in the primary tumors.

[1]  A. Børresen-Dale,et al.  Re‐expression of E‐cadherin, α‐catenin and β‐catenin, but not of γ‐catenin, in metastatic tissue from breast cancer patients , 2000 .

[2]  W. Bodmer,et al.  Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Y. Doki,et al.  Aberrant expression and phosphorylation of beta-catenin in human colorectal cancer. , 1998, British Journal of Cancer.

[4]  P. McCrea,et al.  Phosphorylation of beta-catenin and epidermal growth factor receptor by intestinal trefoil factor. , 1997, Laboratory investigation; a journal of technical methods and pathology.

[5]  J. Palazzo,et al.  Reciprocity between membranous and nuclear expression of β-catenin in colorectal tumours , 1997, Virchows Archiv.

[6]  I. El-Hariry,et al.  Expression of E-cadherin-associated molecules (alpha-, beta-, and gamma-catenins and p120) in colorectal polyps. , 1997, The American journal of pathology.

[7]  J. Palazzo,et al.  Reduced expression of molecules of the cadherin/catenin complex in the transition from colorectal adenoma to carcinoma. , 1997, Anticancer research.

[8]  K. Kinzler,et al.  Constitutive Transcriptional Activation by a β-Catenin-Tcf Complex in APC−/− Colon Carcinoma , 1997, Science.

[9]  S. Hiscox,et al.  Expression of E-cadherin, alpha, beta and gamma-catenin in human colorectal cancer. , 1997, Anticancer research.

[10]  Michael Kühl,et al.  Functional interaction of β-catenin with the transcription factor LEF-1 , 1996, Nature.

[11]  P. Polakis,et al.  Wnt-1 regulates free pools of catenins and stabilizes APC-catenin complexes , 1996, Molecular and cellular biology.

[12]  R. Weinel,et al.  Expression and potential role of E-cadherin in pancreatic carcinoma , 1996, International journal of pancreatology : official journal of the International Association of Pancreatology.

[13]  P. Polakis,et al.  Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Y. Niitsu,et al.  Loss of E-cadherin-dependent cell-cell adhesion due to mutation of the beta-catenin gene in a human cancer cell line, HSC-39 , 1995, Molecular and cellular biology.

[15]  S. Hirohashi,et al.  A Truncated β-Catenin Disrupts the Interaction between E-Cadherin and α-Catenin: A Cause of Loss of Intercellular Adhesiveness in Human Cancer Cell Lines , 1994 .

[16]  H. Shiozaki,et al.  Immunohistochemical detection of alpha-catenin expression in human cancers. , 1994, The American journal of pathology.

[17]  J. Arends,et al.  L‐CAM expression in lymph node and liver metastases of colorectal carcinomas , 1994, The Journal of pathology.

[18]  P. McCrea,et al.  Catenins as mediators of the cytoplasmic functions of cadherins , 1993, Journal of Cell Science.

[19]  M. Mareel,et al.  Immunohistochemical analysis of E-cadherin expression in human colorectal tumours. , 1993, Pathology, research and practice.

[20]  G. Stamp,et al.  Loss of cell-cell and cell-matrix adhesion molecules in colorectal cancer. , 1993, British Journal of Cancer.

[21]  W. Isaacs,et al.  Reduction of E-Cadherin Levels and Deletion of the α-Catenin Gene in Human Prostate Cancer Cells , 1993 .

[22]  A. Kinsella,et al.  The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis. , 1993, British Journal of Cancer.

[23]  W. Birchmeier,et al.  E-cadherin expression in primary and metastatic gastric cancer: down-regulation correlates with cellular dedifferentiation and glandular disintegration. , 1993, Cancer research.

[24]  R. Poulsom,et al.  E-cadherin expression in colorectal cancer. An immunocytochemical and in situ hybridization study. , 1993, The American journal of pathology.

[25]  Frans,et al.  Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene , 1993, The Journal of cell biology.

[26]  M. Takeichi,et al.  p60v‐src causes tyrosine phosphorylation and inactivation of the N‐cadherin‐catenin cell adhesion system. , 1993, The EMBO journal.

[27]  M. Takeichi,et al.  Altered expression of E-cadherin in gastric cancer tissues and carcinomatous fluid. , 1992, British Journal of Cancer.

[28]  J. Arends,et al.  L‐Cam expression in normal, premalignant, and malignant colon mucosa , 1992, The Journal of pathology.

[29]  W. Isaacs,et al.  Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. , 1992, Cancer research.

[30]  S. Hirohashi,et al.  Identification of a neural α-catenin as a key regulator of cadherin function and multicellular organization , 1992, Cell.

[31]  H. Shiozaki,et al.  Expression of E-cadherin in normal, benign, and malignant tissues of female genital organs. , 1992, American journal of clinical pathology.

[32]  W. Fiers,et al.  Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role , 1991, Cell.

[33]  Y Shimoyama,et al.  Expression of E- and P-cadherin in gastric carcinomas. , 1991, Cancer research.

[34]  W. Birchmeier,et al.  E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells , 1991, The Journal of cell biology.

[35]  M. Ringwald,et al.  Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[36]  W. Birchmeier,et al.  Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion , 1989, The Journal of cell biology.

[37]  C. Dukes,et al.  The classification of cancer of the rectum , 1980 .

[38]  W. Hohenberger,et al.  Nuclear overexpression of the oncoprotein beta-catenin in colorectal cancer is localized predominantly at the invasion front. , 1998, Pathology, research and practice.

[39]  R. Poulsom,et al.  A robust method for isotopic riboprobe in situ hybridisation to localise mRNAs in routine pathology specimens. , 1998, European journal of histochemistry : EJH.

[40]  S. Jordan,et al.  Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival. , 1997, Gastroenterology.

[41]  M. Monden,et al.  Beta-catenin expression in human cancers. , 1996, The American journal of pathology.

[42]  M. Mareel,et al.  Cancer metastasis: negative regulation by an invasion-suppressor complex. , 1995, Cancer detection and prevention.

[43]  Y. Doki,et al.  E-Cadherin and α-Catenin Expression in Human Esophageal Cancer , 1994 .

[44]  D. L. Sobin,et al.  Histological Typing of Intestinal Tumours , 1989, World Health Organization.