Versatile nanosecond fluorometer employing a boxcar averager

A nanosecond pulse fluorometer which employs a boxcar averager and has the following novel features is described: (a) It achieves a short data acquisition time with a noise reduction factor in excess of 103; (b) corrects for baseline drift and for exciting light intensity fluctuations; and (c) employs intense nanosecond optical pulses which cover the ultraviolet to the far visible wavelength range. Examples of the performance of the fluorometer, including the measurement of sub‐nanosecond fluorescence decay times, are presented. The effect of the variation of the time profiles of the exciting light pulses with their wavelength on the accurate determination of fluorescence decay times has been investigated for a variety of flashing gases over a wide range of pressures.

[1]  R. D. Dyson,et al.  THE MEASUREMENT OF SUB‐NANOSECOND LIFETIMES BY MONOPHOTON FLUOROMETRY , 1972 .

[2]  S. J. Strickler,et al.  Relationship between Absorption Intensity and Fluorescence Lifetime of Molecules , 1962 .

[3]  G. Weill Rendement quantique, temps de vie de fluorescence et heterogeneité des sites de fixation de la proflavine sur le DNA , 1965 .

[4]  Andreas A. Thaer,et al.  Fluorescence Techniques in Cell Biology , 1973, Springer Berlin Heidelberg.

[5]  I. B. Berlman Handbook of flourescence spectra of aromatic molecules , 1971 .

[6]  P. Wahl,et al.  The wavelength dependence of the response of a pulse fluorometer using the single photoelectron counting method , 1974 .

[7]  Colin Lewis,et al.  The Measurement of Short‐Lived Fluorescence Decay Using the Single Photon Counting Method , 1973 .

[8]  J. B. Birks,et al.  Photophysics of aromatic molecules , 1970 .

[9]  H. Klingenberg,et al.  The influence of an intramolecular relaxation process on the frequency-time behaviour or the fluorescence of tetraphenylethylene in highly viscous solutions , 1973 .

[10]  J. Demas,et al.  Evaluation of photoluminescence lifetimes , 1971 .

[11]  J. Birks,et al.  Phase and modulation fluorometer , 1961 .

[12]  J. B. Birks,et al.  The relations between the fluorescence and absorption properties of organic molecules , 1963, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[13]  B. A. Baldwin,et al.  Absorption Intensity and Fluorescence Lifetimes of Molecules , 1964 .

[14]  T. G. Scott,et al.  Synthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. Studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, [reduced acetylpyridineadenine dinucleotide] and simplified synthetic models , 1970 .

[15]  A. Grinvald,et al.  An improvement of nanosecond fluorimeters to overcome drift problems , 1974 .