A Microtiter Plate Assay as a Reliable Method to Assure the Identification and Classification of the Veil-Forming Yeasts during Sherry Wines Ageing

Yeasts involved in veil formation during biological ageing of Sherry wines are mainly Saccharomyces cerevisiae, and they have traditionally been divided into four races or varieties: beticus, cheresiensis, montuliensis and rouxii. Recent progress in molecular biology has led to the development of several techniques for yeast identification, based on similarity or dissimilarity of DNA, RNA or proteins. In view of the latest yeast taxonomy, there are no more races. However, molecular techniques are not enough to understand the real veil-forming yeast diversity and dynamics in Sherry wines. We propose a reliable method, using a microtiter reader, to evaluate the fermentation and assimilation of carbon and nitrogen sources, the osmotolerance and the antibiotic resistance, using 18 S. cerevisiae and 5 non-Saccharomyces yeast strains, to allow correct identification and classification of the yeast strains present in the velum of flor complex.

[1]  J. Ibeas,et al.  Population analysis of biofilm yeasts during fino sherry wine aging in the Montilla-Moriles D.O. region. , 2017, International journal of food microbiology.

[2]  J. Moreno,et al.  Flor Yeast: New Perspectives Beyond Wine Aging , 2016, Front. Microbiol..

[3]  K. Sterflinger,et al.  Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation , 2016, Applied Microbiology and Biotechnology.

[4]  M. Sipiczki.  Overwintering of Vineyard Yeasts: Survival of Interacting Yeast Communities in Grapes Mummified on Vines , 2016, Front. Microbiol..

[5]  G. Cordero-Bueso,et al.  Rapid and not culture-dependent assay based on multiplex PCR-SSR analysis for monitoring inoculated yeast strains in industrial wine fermentations , 2016, Archives of Microbiology.

[6]  L. Rebordinos,et al.  Enological Behaviour of Biofilms Formed by Genetically-Characterized Strains of Sherry Flor Yeast , 2013 .

[7]  H. Alexandre.  Flor yeasts of Saccharomyces cerevisiae--their ecology, genetics and metabolism. , 2013, International journal of food microbiology.

[8]  L. Rebordinos,et al.  Using RFLP-mtDNA for the rapid monitoring of the dominant inoculated yeast strain in industrial wine fermentations. , 2011, International journal of food microbiology.

[9]  C. Kurtzman,et al.  The Yeasts : A Taxonomic Study Ed. 5 , 2011 .

[10]  L. Rebordinos,et al.  Genomic characterization and selection of wine yeast to conduct industrial fermentations of a white wine produced in a SW Spain winery , 2010, Journal of applied microbiology.

[11]  E. Matallana,et al.  Btn2p is involved in ethanol tolerance and biofilm formation in flor yeast. , 2008, FEMS yeast research.

[12]  Daniel J. Kvitek,et al.  Variations in Stress Sensitivity and Genomic Expression in Diverse S. cerevisiae Isolates , 2008, PLoS genetics.

[13]  E. Vaudano,et al.  Discrimination of Saccharomyces cerevisiae wine strains using microsatellite multiplex PCR and band pattern analysis. , 2008, Food microbiology.

[14]  A. Querol,et al.  Authentication and identification of Saccharomyces cerevisiae‘flor’ yeast races involved in sherry ageing , 2004, Antonie van Leeuwenhoek.

[15]  A. Querol,et al.  RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and the differentiation of flor yeasts , 2000, Antonie van Leeuwenhoek.

[16]  A. Querol,et al.  Yeast Population Dynamics during the Fermentation and Biological Aging of Sherry Wines , 2001, Applied and Environmental Microbiology.

[17]  L. Rebordinos,et al.  Characterization of Yeasts Involved in the Biological Ageing of Sherry Wines , 1999 .

[18]  J. Jiménez,et al.  Mitochondrial DNA loss caused by ethanol in Saccharomyces flor yeasts , 1997, Applied and environmental microbiology.

[19]  C. Kurtzman,et al.  Principles and Methods Used in Yeast Classification, and an Overview of Currently Accepted Yeast Genera , 1996 .

[20]  T. Benítez,et al.  Physiological and molecular characterization of flor yeasts: Polymorphism of flor yeast populations , 1995, Yeast.

[21]  M. Malfeito-Ferreira,et al.  LONG‐CHAIN FATTY ACID COMPOSITION AS A CRITERION FOR YEAST DISTINCTION IN THE BREWING INDUSTRY , 1994 .

[22]  A. Querol,et al.  Molecular Monitoring of Wine Fermentations Conducted by Active Dry Yeast Strains , 1992, Applied and environmental microbiology.

[23]  K. Kersters,et al.  Differentiation of Yeast Species Based On Electrophoretic Whole-cell Protein-patterns , 1991 .

[24]  J. P. Walt,et al.  Chapter II – Methods for the isolation, maintenance, classification and identification of yeasts , 1984 .

[25]  Teun Boekhout,et al.  The yeasts : a taxonomic study , 1972 .