Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.

In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by double-strand DNA breaks (DSBs). Meiotic DSBs occur at relatively high frequencies in some genomic regions (hotspots) and relatively low frequencies in others (coldspots). We used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes. Hotspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles. Coldspots were nonrandomly associated with the centromeres and telomeres.

[1]  T. Petes,et al.  Meiosis-specific double-strand DNA breaks at the HIS4 recombination hot spot in the yeast Saccharomyces cerevisiae: control in cis and trans , 1995, Molecular and cellular biology.

[2]  M. Lichten,et al.  Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase , 1993, Molecular and cellular biology.

[3]  T. Petes,et al.  Control of Meiotic Recombination and Gene Expression in Yeast by a Simple Repetitive DNA Sequence That Excludes Nucleosomes , 1999, Molecular and Cellular Biology.

[4]  R. Pearlman,et al.  Chromatin organization at meiosis , 1988, BioEssays : news and reviews in molecular, cellular and developmental biology.

[5]  T. Petes,et al.  Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain. , 1999, Genetics.

[6]  A. Nicolas,et al.  Clustering of meiotic double-strand breaks on yeast chromosome III. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. McCarthy,et al.  Strong Amerind/white sex bias and a possible Sephardic contribution among the founders of a population in northwest Colombia. , 2000, American journal of human genetics.

[8]  H. Y. Steensma,et al.  Enhanced meiotic recombination on the smallest chromosome of Saccharomyces cerevisiae. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[9]  B. Dujon.  The yeast genome project: what did we learn? , 1996, Trends in genetics : TIG.

[10]  H Yoshikawa,et al.  The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI , 1997, Genes to cells : devoted to molecular & cellular mechanisms.

[11]  D. Botstein,et al.  The transcriptional program of sporulation in budding yeast. , 1998, Science.

[12]  G. Roeder,et al.  A yeast acts in (Cis) to inhibit meiotic gene conversion of adjacent sequences , 1988, Cell.

[13]  N. Kleckner,et al.  Meiotic chromosomes: integrating structure and function. , 1999, Annual review of genetics.

[14]  A. Nicolas,et al.  An atypical topoisomerase II from archaea with implications for meiotic recombination , 1997, Nature.

[15]  B. Bainbridge,et al.  Genetics , 1981, Experientia.

[16]  W. L. Fangman,et al.  Replication profile of Saccharomyces cerevisiae chromosome VI , 1997, Genes to cells : devoted to molecular & cellular mechanisms.

[17]  P. Brown,et al.  Exploring the metabolic and genetic control of gene expression on a genomic scale. , 1997, Science.

[18]  M. Lichten,et al.  Use of a Recombination Reporter Insert To Define Meiotic Recombination Domains on Chromosome III ofSaccharomyces cerevisiae , 1999, Molecular and Cellular Biology.

[19]  A. Goldman,et al.  Meiotic recombination hotspots. , 1995, Annual review of genetics.

[20]  M Lichten,et al.  Meiosis-induced double-strand break sites determined by yeast chromatin structure. , 1994, Science.

[21]  T. Petes,et al.  Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[22]  K. Luo,et al.  SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. , 1997, Genes & development.

[23]  M. Lichten,et al.  Factors that affect the location and frequency of meiosis-induced double-strand breaks in Saccharomyces cerevisiae. , 1995, Genetics.

[24]  M. Sans,et al.  Admixture studies in Latin America: from the 20th to the 21st century. , 2000, Human biology.

[25]  Nancy Kleckner,et al.  Cohesins Bind to Preferential Sites along Yeast Chromosome III, with Differential Regulation along Arms versus the Centric Region , 1999, Cell.

[26]  C. Newlon,et al.  Analysis of replication origin function on chromosome III of Saccharomyces cerevisiae. , 1993, Cold Spring Harbor symposia on quantitative biology.

[27]  T. Petes,et al.  The Tn3 beta-lactamase gene acts as a hotspot for meiotic recombination in yeast. , 1991, Genetics.

[28]  Nihon Hassei Seibutsu Gakkai,et al.  Genes to cells , 1996 .

[29]  P. Sharp,et al.  Regional base composition variation along yeast chromosome III: evolution of chromosome primary structure. , 1993, Nucleic acids research.

[30]  P. Detloff,et al.  DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[31]  S. Keeney,et al.  Meiosis-Specific DNA Double-Strand Breaks Are Catalyzed by Spo11, a Member of a Widely Conserved Protein Family , 1997, Cell.

[32]  A. Sherman,et al.  Multiple sites for double‐strand breaks in whole meiotic chromosomes of Saccharomyces cerevisiae. , 1992, The EMBO journal.

[33]  D. Voytas,et al.  Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence. , 1998, Genome research.