Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital

A single strain of a novel ascomycetous yeast species belonging to the genus Candida was isolated from the external ear canal of an inpatient in a Japanese hospital. Analyses of the 26S rDNA D1/D2 domain, nuclear ribosomal DNA ITS region sequences, and chemotaxonomic studies indicated that this strain represents a new species with a close phylogenetic relationship to Candida ruelliae and Candida haemulonii in the Metschnikowiaceae clade. This strain grew well at 40 °C, but showed slow and weak growth at 42 °C. The taxonomic description of Candida auris sp. nov. is proposed (type strain JCM15448T= CBS10913T= DSM21092T).

[1]  P. Saluja,et al.  Candida ruelliae sp. nov., a novel yeast species isolated from flowers of Ruellia sp. (Acanthaceae). , 2008, FEMS yeast research.

[2]  F. Bai,et al.  Candida alocasiicola sp. nov., Candida hainanensis sp. nov., Candida heveicola sp. nov. and Candida musiphila sp. nov., novel anamorphic, ascomycetous yeast species isolated from plants , 2008, Antonie van Leeuwenhoek.

[3]  M. Nei,et al.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. , 2007, Molecular biology and evolution.

[4]  J. Thomson,et al.  Candida gelsemii sp. nov., a yeast of the Metschnikowiaceae clade isolated from nectar of the poisonous Carolina jessamine , 2007, Antonie van Leeuwenhoek.

[5]  M. Takashima,et al.  Candida pseudohaemulonii Sp. Nov., an Amphotericin B‐ and Azole‐Resistant Yeast Species, Isolated from the Blood of a Patient from Thailand , 2006, Microbiology and immunology.

[6]  H. Yamaguchi,et al.  Differentiation of Candida albicans and Candida dubliniensis using a single-enzyme PCR-RFLP method. , 2005, Japanese journal of infectious diseases.

[7]  A. Nagai,et al.  A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball , 2003, Mycoses.

[8]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[9]  K. Seifert,et al.  The yeasts, a taxonomic study , 2007, Antonie van Leeuwenhoek.

[10]  D. Yarrow Methods for the isolation, maintenance and identification of yeasts , 1998 .

[11]  R. Payne,et al.  64 – Candida Berkhout , 1998 .

[12]  T. White Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[13]  Teun Boekhout,et al.  The yeasts : a taxonomic study , 1972 .