Modified membrane-filter procedure for concentration of enteroviruses from tap water

Enteroviruses added to 114 liters of dechlorinated tap water were recovered in a 16-ml sample by a two-stage concentration procedure in which different types of membrane filters were used in each concentration stage. Viruses in tap water at pH 3.5 were first adsorbed to 10-in. (ca. 25.4-cm) epoxy-fiber glass filters (Filterite). Viruses adsorbed to these filters were eluted with a solution of 0.2 M sodium trichloroacetate buffered at pH 9 with 0.2 M lysine. Viruses in this solution were adsorbed to 47-mm asbestos filters (Seitz) without pH adjustment or other modification of the solution. Viruses were recovered from the Seitz filters with 16 ml of either Casitone or fetal calf serum at pH 9. With these procedures ca. 45% of several types of enteroviruses added to 114 liters of tap water could be recovered in the final 16-ml sample.

[1]  M. Sobsey,et al.  Method for recovery of enteric viruses from estuarine sediments with chaotropic agents , 1983, Applied and environmental microbiology.

[2]  S. Farrah,et al.  Influence of salts on electrostatic interactions between poliovirus and membrane filters , 1983, Applied and environmental microbiology.

[3]  L. Ingram,et al.  Effects of chaotropic and antichaotropic agents on elution of poliovirus adsorbed on membrane filters. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[4]  P. Payment,et al.  A simple low cost apparatus for conditioning large volumes of water for virological analysis. , 1980, Canadian journal of microbiology.

[5]  M. Sobsey,et al.  Concentration of poliovirus from tap water using positively charged microporous filters , 1979, Applied and environmental microbiology.

[6]  C. Gerba,et al.  Concentration of enteroviruses from large volumes of turbid estuary water. , 1977, Canadian journal of microbiology.

[7]  B. Fattal,et al.  Organic Flocculation: an Efficient Second-Step Concentration Method for the Detection of Viruses in Tap Water , 1976, Applied and environmental microbiology.

[8]  C. Gerba,et al.  Concentration of viruses from large volumes of tap water using pleated membrane filters , 1976, Applied and environmental microbiology.

[9]  M. Sobsey,et al.  Concentration of Enteroviruses from Large Volumes of Water , 1973, Applied microbiology.

[10]  C. Wallis,et al.  Enterovirus concentration on cellulose membranes. , 1972, Applied microbiology.

[11]  C. Gerba,et al.  Concentration of viruses from water by membrane chromatography. , 1979, Annual review of microbiology.