Membrane phospholipid composition of Caulobacter crescentus

The phospholipid composition of Caulobacter crescentus CB13 and CB15 was determined. The acidic phospholipids, phosphatidylglycerol and cardiolipin, comprise approximately 87% of the total phospholipids. Neither phosphatidylethanolamine nor its precursor phosphatidylserine was detected. The outer and inner membranes of C. crescentus CB13 were separated, and phospholipid analysis revealed heterogeneity with respect to the relative amounts of phosphatidylglycerol and cardiolipin in the two membranes. As has been shown to be the case for other bacterial membranes, the concentration of cardiolipin increases and phosphatidylglycerol decreases as cell cultures enter stationary phase.

[1]  M. Kania,et al.  Identification of acyl phosphatidylglycerol as a minor phospholipid of Pseudomonas BAL-31. , 1976, Biochimica et biophysica acta.

[2]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[3]  R. Lester,et al.  In vitro studies of phospholipid biosynthesis in Saccharomyces cerevisiae. , 1972, Biochimica et biophysica acta.

[4]  G. Marinetti,et al.  [54a] Chromatography of lipids on silica gel-loaded filter paper , 1969 .

[5]  N. Agabian,et al.  Caulobacter crescentus cell envelope: effect of growth conditions on murein and outer membrane protein composition , 1978, Journal of bacteriology.

[6]  J E Gander,et al.  Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. , 1972, The Journal of biological chemistry.

[7]  A. de Siervo,et al.  Biosynthesis of cardiolipin in the membranes of Micrococcus lysodeikticus. , 1971, Biochimica et biophysica acta.

[8]  J. Cronan.  Phospholipid Alterations During Growth of Escherichia coli , 1968, Journal of bacteriology.

[9]  R. C. Johnson,et al.  Isolation of spontaneously derived mutants of Caulobacter crescentus. , 1977, Genetics.

[10]  D. L. Diedrich,et al.  Heterogeneity in Lipid Composition of the Outer Membrane and Cytoplasmic Membrane of Pseudomonas BAL-31 , 1974 .

[11]  L. Shapiro.  Differentiation in the Caulobacter cell cycle. , 1976, Annual review of microbiology.

[12]  G. Ames.  Lipids of Salmonella typhimurium and Escherichia coli: Structure and Metabolism , 1968, Journal of bacteriology.

[13]  R. Franklin,et al.  Structure and synthesis of a lipid-containing bacteriophage. V. Phospholipids of the host BAL-31 and of the bacteriophage PM2. , 1971, Virology.

[14]  Jean M. Schmidt,et al.  Fatty Acid Composition of Caulobacter crescentus , 1974 .

[15]  L. Shapiro,et al.  Effect of Carbon Source and the Role of Cyclic Adenosine 3′,5′-Monophosphate on the Caulobacter Cell Cycle , 1977, Journal of bacteriology.

[16]  J. Shively,et al.  Phospholipids of Thiobacillus thiooxidans , 1967, Journal of bacteriology.

[17]  J. Dittmer,et al.  A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. , 1964, Journal of lipid research.

[18]  P. Hagen,et al.  PHOSPHOLIPIDS OF CLOSTRIDIUM BUTYRICUM. STUDIES ON PLASMALOGEN COMPOSITION AND BIOSYNTHESIS. , 1965, The Journal of biological chemistry.

[19]  C. Hanes,et al.  Separation of the Phosphoric Esters on the Filter Paper Chromatogram , 1949, Nature.