Phylogenetic foundation of spirochetes.

The spirochetes are free-living or host-associated, helical bacteria, some of which are pathogenic to man and animal. Comparisons of 16S rRNA sequences demonstrate that the spirochetes represent a monophyletic phylum within the bacteria. The spirochetes are presently classified in the Class Spirochaetes in the order Spirochetales and are divided into three major phylogenetic groupings, or families. The first family Spirochaetaceae contains species of the genera Borrelia, Brevinema, Cristispira, Spirochaeta, Spironema, and Treponema. The second family Brachyspiraceae contains the genus Brachyspira (Serpulina). The third family Leptospiraceae contains species of the genera Leptonema and Leptospira. Novel spirochetal species, or phylotypes, that can not be presently cultivated in vitro, have been identified from the human oral cavity, the termite gut, and other host-associated or free-living sources. There are now over 200 spirochetal species or phylotypes, of which more than half is presently not cultivable. It is likely that there is still a significant unrecognized spirochetal diversity that should be evaluated.

[1]  T. Jukes.  CHAPTER 24 – Evolution of Protein Molecules , 1969 .

[2]  T. Stanton,et al.  Enumeration and selective isolation of rumen spirochetes , 1979, Applied and environmental microbiology.

[3]  Philip Hugenholtz,et al.  Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity , 1998, Journal of bacteriology.

[4]  F E Dewhirst,et al.  Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis , 1994, Infection and immunity.

[5]  W. Ludwig,et al.  16S rDNA sequence and phylogenetic position of an uncultivated spirochete from the hindgut of the termite Mastotermes darwiniensis Froggatt. , 1994, FEMS microbiology letters.

[6]  R. C. Johnson,et al.  Brevinema andersonii gen. nov., sp. nov., an infectious spirochete isolated from the short-tailed shrew (Blarina brevicauda) and the white-footed mouse (Peromyscus leucopus). , 1995, International journal of systematic bacteriology.

[7]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[8]  B. Paster,et al.  Involvement of periplasmic fibrils in motility of spirochetes , 1980, Journal of bacteriology.

[9]  S. Block,et al.  Morphology and dynamics of protruding spirochete periplasmic flagella , 1992, Journal of bacteriology.

[10]  T. Schmidt,et al.  Phylogenetic diversity of termite gut spirochaetes. , 1999, Environmental microbiology.

[11]  Strain Cip,et al.  Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 40. , 1992, International journal of systematic bacteriology.

[12]  Yves Van de Peer,et al.  TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment , 1994, Comput. Appl. Biosci..

[13]  R. Ericson,et al.  The diversity of periodontal spirochetes by 16S rRNA analysis. , 2000, Oral microbiology and immunology.

[14]  B. J. Newton,et al.  Isolation and characterization of Borrelia burgdorferi from Illinois Ixodes dammini , 1991, Journal of clinical microbiology.

[15]  M. J. Kennedy,et al.  Isolation, Oxygen Sensitivity, and Virulence of NADH Oxidase Mutants of the Anaerobic Spirochete Brachyspira(Serpulina) hyodysenteriae, Etiologic Agent of Swine Dysentery , 1999, Applied and Environmental Microbiology.

[16]  N. Pace,et al.  The Analysis of Natural Microbial Populations by Ribosomal RNA Sequences , 1986 .

[17]  Phylogenetic relationships of symbiotic spirochetes in the gut of diverse termites. , 1999, FEMS microbiology letters.

[18]  C. Woese,et al.  Phylogenetic analysis of the spirochetes , 1991, Journal of bacteriology.

[19]  Floyd E. Dewhirst,et al.  Taxonomy of spirochetes , 2000 .

[20]  D J Trott,et al.  Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. , 1996, International journal of systematic bacteriology.

[21]  James R. Cole,et al.  A new version of the RDP (Ribosomal Database Project) , 1999, Nucleic Acids Res..

[22]  D. Postic,et al.  Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens. , 1998, International journal of systematic bacteriology.

[23]  Ira Schwartz,et al.  Molecular Typing of Borrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications , 1999, Clinical Microbiology Reviews.

[24]  N. Pace,et al.  Identifying microbial diversity in the natural environment: a molecular phylogenetic approach. , 1996, Trends in biotechnology.

[25]  F. Dewhirst,et al.  Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization , 1998 .

[26]  S. Leschine,et al.  Rifampin-resistant RNA polymerase in spirochetes , 1986 .

[27]  L. Poulsen,et al.  Phylogenetic position of the spirochetal genus Cristispira , 1996, Applied and environmental microbiology.

[28]  S. Leschine,et al.  Rifampin as a Selective Agent for Isolation of Oral Spirochetes , 1980, Journal of clinical microbiology.

[29]  L. Poulsen,et al.  Phylogeny of not-yet-cultured spirochetes from termite guts , 1996, Applied and environmental microbiology.