RESEARCH ARTICLE HOST-GUEST INCLUSION COMPLEX OF B-CYCLODEXTRIN AND CEPHALEXIN AND ITS ANALYTICAL APPLICATION

The host–guest inclusion complex between β-cyclodextrin (β-CD) and cephalexin was investigated spectrofluorometrically. The results showed that β-CD reacted with cephalexin to form 1:1 stoichiometry host-guest inclusion complex. Inclusion constant (K = 5.33×10 2 L mol -1 ) was ascertained by the typical double reciprocal plots in pH 5.0 buffer solution. Furthermore, the thermodynamic parameters ( H°, S° and G°) associated with the inclusion process were also determined. The factors affecting the inclusion complex formation were carefully studied, incorporated in the procedure and optimized. A spectrofluorimetric method for the determination of cephalexin in solution in the presence of β-CD was developed based on the remarkable enhancement of the fluorescence intensity of cephalexin. Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.9992) were in the concentration range of 1–10 µg/mL was obtained. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.001 µg/mL and 0.036 µg/mL, respectively. The recovery was in the range of (104–124.5) %. The method has been successfully applied to the determination of cephalexin in pharmaceutical formulation with good accuracy. Key word: cephalexin, inclusion complex; β-cyclodextrin; spectrofluorimetric

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