Bioanalytical and chemical evaluation of disinfection by-products in swimming pool water.

Pool water disinfection is vital to prevent microbial pathogens. However, potentially hazardous disinfection by-products (DBP) are formed from the reaction between disinfectants and organic/inorganic precursors. The aim of this study was to evaluate the presence of DBPs in various swimming pool types in Brisbane, Australia, including outdoor, indoor and baby pools, and the dynamics after a complete water renewal. Chemical analysis of 36 regulated and commonly found DBPs and total adsorbable organic halogens as well as in vitro bioassays targeting cytotoxicity, oxidative stress and genotoxicity were used to evaluate swimming pool water quality. Dichloroacetic acid and trichloroacetic acid dominated in the pool water samples with higher levels (up to 2600 μg/L) than the health guideline values set by the Australian Drinking Water Guidelines (100 μg/L). Chlorinated DBPs occurred at higher concentrations compared to tap water, while brominated DBPs decreased gradually with increasing pool water age. Biological effects were expressed as chloroacetic acid equivalent concentrations and compared to predicted effects from chemical analysis and biological characterisation of haloacetic acids. The quantified haloacetic acids explained 35-118% of the absorbable organic halogens but less than 4% of the observed non-specific toxicity (cytotoxicity), and less than 1% of the observed oxidative stress response and genotoxicity. While the DBP concentrations in Australian pools found in this study are not likely to cause any adverse health effect, they are higher than in other countries and could be reduced by better hygiene of pool users, such as thorough showering prior to entering the pool and avoiding urination during swimming.

[1]  M. Plewa,et al.  Comparative genotoxicity of nitrosamine drinking water disinfection byproducts in Salmonella and mammalian cells. , 2012, Mutation research.

[2]  Ann Richard,et al.  Evaluation of high-throughput genotoxicity assays used in profiling the US EPA ToxCast chemicals. , 2009, Regulatory toxicology and pharmacology : RTP.

[3]  B. Escher,et al.  Bioanalytical and chemical assessment of the disinfection by-product formation potential: role of organic matter. , 2013, Water research.

[4]  H. Albrechtsen,et al.  Effect of pH on the formation of disinfection byproducts in swimming pool water--is less THM better? , 2012, Water research.

[5]  Simon Toze,et al.  Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway. , 2012, Journal of environmental monitoring : JEM.

[6]  Rolf Altenburger,et al.  Benchmarking organic micropollutants in wastewater, recycled water and drinking water with in vitro bioassays. , 2014, Environmental science & technology.

[7]  Simon Toze,et al.  Bioanalytical tools for the evaluation of organic micropollutants during sewage treatment, water recycling and drinking water generation. , 2011, Water research.

[8]  Xiangru Zhang,et al.  Characterization of high molecular weight disinfection byproducts resulting from chlorination of aquatic humic substances. , 2002, Environmental science & technology.

[9]  Rolf Altenburger,et al.  Most oxidative stress response in water samples comes from unknown chemicals: the need for effect-based water quality trigger values. , 2013, Environmental science & technology.

[10]  Frederic D.L. Leusch,et al.  Bioanalytical Tools in Water Quality Assessment , 2011 .

[11]  Xiangru Zhang,et al.  Decomposition of trihaloacetic acids and formation of the corresponding trihalomethanes in drinking water. , 2002, Water research.

[12]  M. Kogevinas,et al.  Genotoxic Effects in Swimmers Exposed to Disinfection By-products in Indoor Swimming Pools , 2010, Environmental health perspectives.

[13]  D. DeMarini,et al.  Occurrence, genotoxicity, and carcinogenicity of regulated and emerging disinfection by-products in drinking water: a review and roadmap for research. , 2007, Mutation research.

[14]  O. Thomas,et al.  Determinants of chlorination by-products in indoor swimming pools. , 2011, International journal of hygiene and environmental health.

[15]  Andreas Natsch,et al.  The Nrf2-Keap1-ARE toxicity pathway as a cellular sensor for skin sensitizers--functional relevance and a hypothesis on innate reactions to skin sensitizers. , 2010, Toxicological sciences : an official journal of the Society of Toxicology.

[16]  Soohyung Lee,et al.  Formation of disinfection by-products in chlorinated swimming pool water. , 2002, Chemosphere.

[17]  S. Richardson,et al.  Occurrence of a new generation of disinfection byproducts. , 2006, Environmental science & technology.

[18]  Beatrice Gralton,et al.  Washington DC - USA , 2008 .

[19]  Ana Deletic,et al.  Toxicity characterization of urban stormwater with bioanalytical tools. , 2013, Water research.

[20]  F. Frimmel,et al.  Swimming pool water--fractionation and genotoxicological characterization of organic constituents. , 2005, Water research.

[21]  P. Duerksen-Hughes,et al.  p53 induction as a genotoxic test for twenty-five chemicals undergoing in vivo carcinogenicity testing. , 1999, Environmental health perspectives.

[22]  Ramesh D. Kashinkunti,et al.  Differential toxicity of drinking water disinfected with combinations of ultraviolet radiation and chlorine. , 2012, Environmental science & technology.

[23]  M. Andersen,et al.  Organic extract contaminants from drinking water activate Nrf2-mediated antioxidant response in a human cell line. , 2013, Environmental science & technology.

[24]  M. Plewa,et al.  Biological mechanism for the toxicity of haloacetic acid drinking water disinfection byproducts. , 2011, Environmental science & technology.

[25]  J. E. Simmons,et al.  Mammalian cell cytotoxicity and genotoxicity of the haloacetic acids, a major class of drinking water disinfection by‐products , 2010, Environmental and molecular mutagenesis.

[26]  M. Xia,et al.  Human cell toxicogenomic analysis linking reactive oxygen species to the toxicity of monohaloacetic acid drinking water disinfection byproducts. , 2013, Environmental science & technology.

[27]  Manuel J Rodriguez,et al.  Variability of chlorination by-product occurrence in water of indoor and outdoor swimming pools. , 2013, Water research.

[28]  F. Frimmel,et al.  Trichloramine in swimming pools--formation and mass transfer. , 2011, Water research.

[29]  M. Jun,et al.  Production of various disinfection byproducts in indoor swimming pool waters treated with different disinfection methods. , 2010, International journal of hygiene and environmental health.

[30]  Debra Silverman,et al.  Bladder cancer and exposure to water disinfection by-products through ingestion, bathing, showering, and swimming in pools. , 2006, American journal of epidemiology.

[31]  B. Escher,et al.  Headspace-free setup of in vitro bioassays for the evaluation of volatile disinfection by-products. , 2013, Chemical research in toxicology.

[32]  H. Shinagawa,et al.  Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens. , 1985, Mutation research.

[33]  J. Keller,et al.  Understanding the operational parameters affecting NDMA formation at Advanced Water Treatment Plants. , 2011, Journal of hazardous materials.

[34]  T. Karanfil,et al.  Disinfection byproducts in swimming pool: occurrences, implications and future needs. , 2014, Water research.

[35]  M. Gallego,et al.  Haloacetic acids in swimming pools: swimmer and worker exposure. , 2011, Environmental science & technology.

[36]  K. Cammann,et al.  False results in headspace—gas chromatographic analysis of trihalomethanes in swimming pool water due to elevated headspace temperatures , 1993 .

[37]  D. Reckhow,et al.  Comparison of disinfection byproduct formation from chlorine and alternative disinfectants. , 2007, Water research.

[38]  M. Plewa,et al.  Mammalian cell cytotoxicity and genotoxicity analysis of drinking water disinfection by‐products , 2002, Environmental and molecular mutagenesis.

[39]  Beate I Escher,et al.  Mixture effects of organic micropollutants present in water: towards the development of effect-based water quality trigger values for baseline toxicity. , 2013, Water research.

[40]  Jonathan Bridgeman,et al.  Predicting chlorine decay and THM formation in water supply systems , 2011 .

[41]  Christian Zwiener,et al.  Drowning in disinfection byproducts? Assessing swimming pool water. , 2007, Environmental science & technology.

[42]  F. Dossier-Berne,et al.  Concentration levels of urea in swimming pool water and reactivity of chlorine with urea. , 2011, Water research.

[43]  M. Plewa,et al.  Genotoxicity of water concentrates from recreational pools after various disinfection methods. , 2010, Environmental science & technology.

[44]  C Roland Wolf,et al.  Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents. , 2006, Cancer research.

[45]  S. Judd,et al.  The fate of chlorine and organic materials in swimming pools. , 2003, Chemosphere.

[46]  U. Stenius,et al.  Re: Yang, J. and Duerksen-Hughes, P. (1998) A new approach to identifying genotoxic carcinogens: p53 induction as an indicator of genotoxic damage. Carcinogenesis, 19, 1117-1125. , 1999, Carcinogenesis.

[47]  L. Attardi,et al.  Deconstructing p53 transcriptional networks in tumor suppression. , 2012, Trends in cell biology.

[48]  S. Richardson,et al.  Comparative mammalian cell toxicity of N-DBPs and C-DBPs , 2008 .

[49]  P. Hartemann,et al.  Health effects of disinfection by-products in chlorinated swimming pools. , 2011, International journal of hygiene and environmental health.

[50]  T. Karanfil,et al.  Formation of disinfection by-products in indoor swimming pool water: the contribution from filling water natural organic matter and swimmer body fluids. , 2011, Water research.

[51]  S. Richardson,et al.  Halonitromethane drinking water disinfection byproducts: chemical characterization and mammalian cell cytotoxicity and genotoxicity. , 2004, Environmental science & technology.

[52]  Nathaniel Rothman,et al.  Polymorphisms in GSTT1, GSTZ1, and CYP2E1, Disinfection By-products, and Risk of Bladder Cancer in Spain , 2010, Environmental health perspectives.

[53]  M. Bartkow,et al.  Bioanalytical assessment of the formation of disinfection byproducts in a drinking water treatment plant. , 2012, Environmental science & technology.

[54]  M. Rebhun,et al.  Formation and distribution of haloacetic acids, THM and TOX in chlorination of bromide-rich lake water , 1993 .

[55]  Steven O Simmons,et al.  Cellular stress response pathway system as a sentinel ensemble in toxicological screening. , 2009, Toxicological sciences : an official journal of the Society of Toxicology.

[56]  William A. Mitch,et al.  Comparative Mammalian cell cytotoxicity of water concentrates from disinfected recreational pools. , 2011, Environmental science & technology.

[57]  Kees Meliefste,et al.  What’s in the Pool? A Comprehensive Identification of Disinfection By-products and Assessment of Mutagenicity of Chlorinated and Brominated Swimming Pool Water , 2010, Environmental health perspectives.