Genotyping of the Helicobacter pylori isolates of raw milk and traditional dairy products

Notwithstanding the substantial clinical impact of Helicobacter pylori , its convinced routes of transmission and sources have not been reported. Based on the quarrelsome hypothesis, foods and especially dairy products play an authoritative role in the transmission of H. pylori to humans. The current investigation was done to study the prevalence rate and distribution of vacA genotypes in the H. pylori strains isolated from the raw milk and traditional dairy products. Three-hundred milk and dairy samples were collected and directly transported to laboratory. Samples were cultured and H. pylori isolates were approved using the 16s rRNAbased PCR amplification. Positive strains were tested for distribution of vacA genotypes using the multiplex-PCR. Sixty out of 300 samples (20%) harbored H. pylori. Prevalence of H. pylori in milk and traditional dairy products were 38.75% and 13.18%, respectively. Ovine milk (45%) and traditional cheese (40%) had the highest prevalence of H. pylori. VacAs1a (91.66%), vacAm1a (61.61%) vacAs2 (36.66%) and vacAm2 (31.66%) were the most commonly detected genotypes. Ovine milk and traditional cheese had the most diverse genotypes. S1am1a (41.66%), s2m1a (25%), s1am2 (16.66%) and s2m2 (13.33%) were the most commonly detected combined genotypes. Raw milk and traditional dairy products are latent sources of H. pylori . Similarity in the genotyping pattern of H. pylori strains of various samples represents their similar sources of infection. Further studies are required to found the exact sources of H. pylori strains in raw milk and traditional dairy products.

[1]  M. Camorlinga-Ponce,et al.  Helicobacter pylori vacA s1m1 genotype but not cagA or babA2 increase the risk of ulcer and gastric cancer in patients from Southern Mexico , 2017, Gut Pathogens.

[2]  E. Rahimi,et al.  VacA and cagA genotypes of Helicobacter pylori isolated from raw meat in Isfahan province, Iran , 2017, Veterinary research forum : an international quarterly journal.

[3]  F. Ghorbani,et al.  Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items , 2016 .

[4]  F. Carneiro,et al.  Helicobacter pylori vacA Genotypes in Chronic Gastritis and Gastric Carcinoma Patients from Macau, China , 2016, Toxins.

[5]  E. Saeidi,et al.  vacA Genotype Status of Helicobacter pylori Isolated from Foods with Animal Origin , 2016, BioMed research international.

[6]  E. Rahimi,et al.  VacA, cagA, iceA and oipA genotypes status and antimicrobial resistance properties of Helicobacter pylori isolated from various types of ready to eat foods , 2016, Annals of Clinical Microbiology and Antimicrobials.

[7]  A. El-Gohary,et al.  Epidemiological Study on H. pylori in Cattle and Its Milk with SpecialReference to its Zoonotic Importance , 2015 .

[8]  F. S. Tameshkel,et al.  Prevalence of Helicobacter pyloriinIranian milk and dairy products using culture and ureC based-PCR techniques , 2015 .

[9]  H. Momtaz,et al.  Milk of livestock as a possible transmission route of Helicobacter pylori infection , 2015, Gastroenterology and hepatology from bed to bench.

[10]  E. Rahimi,et al.  Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran , 2014, Journal of Venomous Animals and Toxins including Tropical Diseases.

[11]  A. Memon,et al.  Association between Helicobacter pylori genotypes and severity of chronic gastritis, peptic ulcer disease and gastric mucosal interleukin-8 levels: Evidence from a study in the Middle East , 2014, Gut Pathogens.

[12]  F. Khamesipour,et al.  Helicobacter pylori in Vegetables and Salads: Genotyping and Antimicrobial Resistance Properties , 2014, BioMed research international.

[13]  V. Y. Miendje Deyi,et al.  Vacuolating Cytotoxin Genotypes Are Strong Markers of Gastric Cancer and Duodenal Ulcer-Associated Helicobacter pylori Strains: a Matched Case-Control Study , 2014, Journal of Clinical Microbiology.

[14]  E. Rahimi,et al.  Detection of Helicobacter pylori in Various Types of Vegetables and Salads , 2014, Jundishapur journal of microbiology.

[15]  B. Marshall,et al.  Comparative Analysis of the Full Genome of Helicobacter pylori Isolate Sahul64 Identifies Genes of High Divergence , 2013, Journal of bacteriology.

[16]  Bingrong Liu,et al.  Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes and correlation with clinical outcome , 2012, Experimental and therapeutic medicine.

[17]  E. Rahimi,et al.  Molecular Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Ruminant and Donkey Raw Milk Samples and Traditional Dairy Products in Iran , 2012, TheScientificWorldJournal.

[18]  K. Bhat,et al.  Role of Helicobacter pylori specific heat shock protein-60 antibodies in the aetiology of coronary artery disease , 2012 .

[19]  E. Rahimi,et al.  Detection of Helicobacter pylori in bovine, buffalo, camel, ovine, and caprine milk in Iran. , 2012, Foodborne pathogens and disease.

[20]  L. Bravo,et al.  Non‐invasive Genotyping of Helicobacter pylori cagA, vacA, and hopQ from Asymptomatic Children , 2012, Helicobacter.

[21]  Janice S. Chen,et al.  Non-invasive genotyping of transgenic animals using fecal DNA , 2012, Lab Animal.

[22]  Savita Sambashivaiah,et al.  Helicobacter Pylori in periodontal pockets of chronic periodontitis patients with and without type II diabetes mellitus: a randomized controlled trial , 2011 .

[23]  E. Rahimi,et al.  Helicobacter pylori as a zoonotic infection: the detection of H. pylori antigens in the milk and faeces of cows , 2011, Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences.

[24]  D. Graham,et al.  Helicobacter pylori Virulence Factor Genotypes in Children in the United States: Clues about Genotype and Outcome Relationships , 2010, Journal of Clinical Microbiology.

[25]  J. Vítor,et al.  Transmission pathway of Helicobacter pylori: does food play a role in rural and urban areas? , 2010, International journal of food microbiology.

[26]  Y. Yamaoka,et al.  vacA genotypes of Helicobacter pylori in relation to cagA status and clinical outcomes in Iranian populations. , 2008, Japanese journal of infectious diseases.

[27]  S. Fujimura,et al.  Detection of Helicobacter pylori in cow's milk , 2002, Letters in applied microbiology.

[28]  Debra,et al.  Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals , 1991, Journal of clinical microbiology.

[29]  R. Cali.  Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children , 2014 .

[30]  A. G. Herrera.  Helicobacter pylori and food products: a public health problem. , 2004, Methods in molecular biology.