Synergistic Effects of Trichoderma harzianum, 1,3 Dichloropropene and Organic Matter in Controlling the Root-Knot Nematode Meloidogyne incognita on Tomato

Environmental concerns raised by synthetic nematicides are encouraging integrated management strategies based on their combination with non-chemical control tools, such as biocontrol agents and/or organic amendments. In this study, the combination of the fumigant 1,3-dichloropropene (1,3-D) with a commercial formulation of the biocontrol agent Trichoderma harzianum (TH) and an organic fertilizer (OF) was investigated in two consecutive tomato crops for its effect on the root-knot nematode Meloidogyne incognita and plant growth and yield. The application of 1,3-D was only performed on the first crop, while TH and OF were provided to both crops. Almost all treatments significantly reduced nematode infestation in both crops, though the greatest nematicidal effect was caused by a combination of the three products. The treatment with 1,3-D limited its nematicidal efficacy to the first crop only. Fumigant integration with TH and OF also resulted in the greatest increases of plant growth and yield. Therefore, the integrated management of root-knot nematodes with a soil fumigant, a bionematicide as T. harzianum and a source of organic matter demonstrated effective nematode suppression though limiting the number of chemical applications.

[1]  Pratik Doshi,et al.  Composted Municipal Green Waste Infused with Biocontrol Agents to Control Plant Parasitic Nematodes—A Review , 2021, Microorganisms.

[2]  T. Grey,et al.  Root-knot nematode management for pepper and squash rotations using plasticulture systems with fumigants and non-fumigant nematicides , 2021, Crop Protection.

[3]  R. Kataoka,et al.  Effect of co-application of Trichoderma spp. with organic composts on plant growth enhancement, soil enzymes and fungal community in soil , 2021, Archives of Microbiology.

[4]  A. Cao,et al.  Bio-activation of soil with beneficial microbes after soil fumigation reduces soil-borne pathogens and increases tomato yield. , 2021, Environmental pollution.

[5]  T. Mukhtar,et al.  Bioefficacy of Trichoderma Species Against Javanese Root-Knot Nematode, Meloidogyne javanica, in Green Gram , 2021 .

[6]  Xiaoyu Liu,et al.  Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita , 2020, BMC microbiology.

[7]  M. Malinconico,et al.  Trichoderma spp. and a carob (Ceratonia siliqua) galactomannan to control the root-knot nematode Meloidogyne incognita on tomato plants , 2020 .

[8]  E. Rosskopf,et al.  Organic Amendments for Pathogen and Nematode Control. , 2020, Annual review of phytopathology.

[9]  W. Bert,et al.  Compost is a carrier medium for Trichoderma harzianum , 2020, BioControl.

[10]  Baoqiang Hao,et al.  Organic fertilizer improves soil fertility and restores the bacterial community after 1,3-dichloropropene fumigation. , 2020, The Science of the total environment.

[11]  J. Poveda,et al.  Biological Control of Plant-Parasitic Nematodes by Filamentous Fungi Inducers of Resistance: Trichoderma, Mycorrhizal and Endophytic Fungi , 2020, Frontiers in Microbiology.

[12]  R. Jackson,et al.  Endophytes vs tree pathogens and pests: can they be used as biological control agents to improve tree health? , 2019, European Journal of Plant Pathology.

[13]  M. Lorito,et al.  Integrated management strategies of Meloidogyne incognita and Pseudopyrenochaeta lycopersici on tomato using a Bacillus firmus-based product and two synthetic nematicides in two consecutive crop cycles in greenhouse , 2019, Crop Protection.

[14]  Junling Shi,et al.  Beneficial effects of endophytic fungi colonization on plants , 2019, Applied Microbiology and Biotechnology.

[15]  S. Lumyong,et al.  Use of endophytes as biocontrol agents , 2019, Fungal Biology Reviews.

[16]  F. Grundler,et al.  Parasitic nematodes manipulate plant development to establish feeding sites. , 2018, Current opinion in microbiology.

[17]  P. Castillo,et al.  Anatomical Alterations in Plant Tissues Induced by Plant-Parasitic Nematodes , 2017, Front. Plant Sci..

[18]  Jia Liu,et al.  Mechanisms and Characterization of Trichoderma longibrachiatum T6 in Suppressing Nematodes (Heterodera avenae) in Wheat , 2017, Front. Plant Sci..

[19]  B. Kowalska,et al.  Application of organic waste material overgrown with Trichoderma atroviride as a control strategy for Sclerotinia sclerotiorum and Chalara thielavioides in soil , 2017 .

[20]  Rajan Chaurasia,et al.  Harnessing Biocontrol Potential of Trichoderma harzianum for Control of Meloidogyne incognita in Tomato , 2017 .

[21]  C. Pieterse,et al.  Shifting from priming of salicylic acid- to jasmonic acid-regulated defences by Trichoderma protects tomato against the root knot nematode Meloidogyne incognita. , 2017, The New phytologist.

[22]  M. Moosavi,et al.  Effect of soil texture and its organic content on the efficacy of Trichoderma harzianum (MIAU 145 C) in controlling Meloidogyne javanica and stimulating the growth of kidney beans , 2017 .

[23]  Céline Bellard,et al.  Massive yet grossly underestimated global costs of invasive insects , 2016, Nature Communications.

[24]  A. Alix,et al.  Integrating ecosystem services into crop protection and pest management: Case study with the soil fumigant 1,3‐dichloropropene and its use in tomato production in Italy , 2016, Integrated environmental assessment and management.

[25]  J. Larsen,et al.  Ecological functions of Trichoderma spp. and their secondary metabolites in the rhizosphere: interactions with plants. , 2016, FEMS microbiology ecology.

[26]  K. Saikkonen,et al.  Endophytic phyllosphere fungi and nutrient cycling in terrestrial ecosystems , 2015 .

[27]  Kee-Choon Park,et al.  Effects of dazomet on soil organisms and recolonisation of fumigated soil , 2014 .

[28]  Y. Gan,et al.  The parasitic and lethal effects of Trichoderma longibrachiatum against Heterodera avenae , 2014 .

[29]  Johannes Helder,et al.  Top 10 plant-parasitic nematodes in molecular plant pathology. , 2013, Molecular plant pathology.

[30]  Liping Lei,et al.  Nematicidal effect of volatiles produced by Trichoderma sp. , 2012 .

[31]  A. Ragozzino,et al.  Interactions between nematodes and fungi: a concise review , 2012 .

[32]  J. Coosemans,et al.  Biocontrol potential of native Trichoderma isolates against root-knot nematodes in West African vegetable production systems , 2011 .

[33]  M. Lorito,et al.  Translational research on Trichoderma: from 'omics to the field. , 2010, Annual review of phytopathology.

[34]  I. Chet,et al.  Improved attachment and parasitism of Trichoderma on Meloidogyne javanica in vitro , 2009, European Journal of Plant Pathology.

[35]  M. Gullino,et al.  Application of an emulsifiable mixture of 1,3-dichloropropene and chloropicrin against root knot nematodes and soilborne fungi for greenhouse tomatoes in Italy , 2006 .

[36]  I. Chet,et al.  MECHANISMS AND IMPROVED BIOCONTROL OF THE ROOT-KNOT NEMATODES BY TRICHODERMA SPP. , 2005 .

[37]  P. Timper,et al.  Soil fumigation and oxamyl drip applications for nematode and insect control in vegetable plasticulture , 2004 .

[38]  I. Chet,et al.  Biological Control of the Root-Knot Nematode Meloidogyne javanica by Trichoderma harzianum. , 2001, Phytopathology.

[39]  T. Bongers,et al.  Feeding habits in soil nematode families and genera-an outline for soil ecologists. , 1993, Journal of nematology.

[40]  R. Chand,et al.  Efficacy of Trichoderma viride and T. harzianum culture filtrate on egg hatching and larval mortality of root-knot nematode, Meloidogyne incognita , 2021, Annals of Plant Protection Sciences.

[41]  R. Ansari,et al.  Role of Trichoderma spp. in the Management of Plant-Parasitic Nematodes Infesting Important Crops , 2020 .

[42]  J. Bhatt,et al.  Bio-efficacy of Trichoderma viride against the root-knot nematode (Meloidogyne incognita) in tomato plant , 2018 .

[43]  Bijendra Singh,et al.  Nematodes: A Threat to Sustainability of Agriculture , 2015 .

[44]  I. Chet,et al.  Plant-beneficial effects of Trichoderma and of its genes. , 2012, Microbiology.

[45]  I. Chet,et al.  Trichoderma as a Biological Control Agent , 2011 .

[46]  I. Barber,et al.  The soil ecotoxicology of 1,3-dichloropropene under commercial growing conditions. , 2008, Communications in agricultural and applied biological sciences.

[47]  K. Sivasithamparam,et al.  Trichoderma–plant–pathogen interactions , 2008 .

[48]  L. Leandro,et al.  Population dynamics of Trichoderma in fumigated and compost-amended soil and on strawberry roots , 2007 .

[49]  Gary E. Harman,et al.  Trichoderma species — opportunistic, avirulent plant symbionts , 2004, Nature Reviews Microbiology.

[50]  C. R. Howell.  Mechanisms Employed by Trichoderma Species in the Biological Control of Plant Diseases: The History and Evolution of Current Concepts. , 2003, Plant disease.