Antibacterial and synergistic effects of Nardostachytis rhizoma extracts on methicillin-resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is a serious clinical problem worldwide. Few new drugs are available against MRSA, because it has the ability to acquire resistance to most antibiotics which consequently increases the cost of medication. In the present study, the antibacterial activity of Nardostachytis rhizoma was investigated. The most effective method is to develop antibiotics from the natural products without having any toxic or side effects. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases. The use of two drugs in combination is a good alternative to slow the process of developing drug resistance and to restore the effectiveness of drugs that are no longer prescribed. Combination therapy is the most commonly recommended empirical treatment for bacterial infections in intensive care units, where monotherapy may not be effective against all potential pathogens, and for preventing the emergence of resistant. Five clinical isolates (MRSA) were obtained from five different patients at Wonkwang University Hospital (Iksan, South Korea). The other two strains were S. aureus ATCC 33591 (methicillin-resistant strain) and S. aureus ATCC 25923 (methicillin-susceptible strain). Antibacterial activity (minimal inhibitory concentrations, MICs) was determined by broth dilution method, disc diffusion method, MTT test and checkerboard dilution test. Antimicrobial activity of n-hexane fraction of N. rhizoma was significant. Against the seven strains, the disc diffusion test was in the range of 14 to 18 mm and had a MICs ranging from 31.25 to 125 ig/ml. FICI values for n-hexane fraction (HFL) of N. rhizome + ampicillin (AM) and HFL + oxacillin (OX) were 0.1875 and 0.078125-0.09375, showing the increase of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effective combinations could be new promising agents in the management of MRSA and MSSA. Key words : Nardostachytis rhizoma, synergism, antibacterial, methicillin-resistant Staphylococcus aureus (MRSA).

[1]  Mohammad Wahid Ansari,et al.  The legal status of in vitro embryos , 2014 .

[2]  N. Bahtiti.  Chemical Analysis and Biological Activity of Jordanian Chamomile Extracts , 2012 .

[3]  Jihua Wang,et al.  Chemical Analysis and Biological Activity of the Essential Oils of Two Valerianaceous Species from China: Nardostachys chinensis and Valeriana officinalis , 2010, Molecules.

[4]  M. Falagas,et al.  Synergy of fosfomycin with other antibiotics for Gram-positive and Gram-negative bacteria , 2010, European Journal of Clinical Pharmacology.

[5]  S. Dancer.  The effect of antibiotics on methicillin-resistant Staphylococcus aureus. , 2007, The Journal of antimicrobial chemotherapy.

[6]  Yujun Shi,et al.  A new method for antimicrobial susceptibility testing of in vitro-cultured bacteria by means of resonance light scattering technique. , 2008, Journal of microbiology and biotechnology.

[7]  L. Drago,et al.  In vitro evaluation of antibiotics' combinations for empirical therapy of suspected methicillin resistant Staphylococcus aureus severe respiratory infections , 2007, BMC infectious diseases.

[8]  L. Bopp,et al.  Antimicrobial Activities of Daptomycin, Vancomycin, and Oxacillin in Human Monocytes and of Daptomycin in Combination with Gentamicin and/or Rifampin in Human Monocytes and in Broth against Staphylococcus aureus , 2007, Antimicrobial Agents and Chemotherapy.

[9]  F. Solórzano-Santos,et al.  Annals of Clinical Microbiology and Antimicrobials Open Access in Vitro Activity Effects of Combinations of Cephalothin, Dicloxacillin, Imipenem, Vancomycin and Amikacin against Methicillin-resistant Staphylococcus Spp. Strains , 2022 .

[10]  I. Ahmad,et al.  Evaluation of anti-methicillin-resistant Staphylococcus aureus (MRSA) activity and synergy of some bioactive plant extracts. , 2006, Biotechnology journal.

[11]  Soojin Lee,et al.  Inhibitory effects of aqueous extracts from nardostachys Chinensis on α‐melanocyte stimulating hormone‐induced melanogenesis in B16F10 cells , 2006 .

[12]  Clinical,et al.  Performance standards for antimicrobial disk susceptibility tests : approved standard , 2006 .

[13]  S. Dastidar,et al.  In vitro and in vivo synergism between tetracycline and the cardiovascular agent oxyfedrine HCl against common bacterial strains. , 2005, Biological & pharmaceutical bulletin.

[14]  F. Odds,et al.  Synergy, antagonism, and what the chequerboard puts between them. , 2003, The Journal of antimicrobial chemotherapy.

[15]  D. Durham,et al.  Baicalin Synergy with β‐Lactam Antibiotics Against Methicillin‐resistant Staphylococcus aureus and Other β‐Lactam‐resistant Strains of S. aureus , 2000 .

[16]  D. Durham,et al.  Baicalin synergy with beta-lactam antibiotics against methicillin-resistant Staphylococcus aureus and other beta-lactam-resistant strains of S. aureus. , 2000, The Journal of pharmacy and pharmacology.

[17]  W. Witte,et al.  Antibiotic resistance in gram-positive bacteria: epidemiological aspects. , 1999, The Journal of antimicrobial chemotherapy.

[18]  H. Miörner,et al.  Evaluation of a colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. , 1998, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[19]  H. Morita,et al.  Cytotoxic sesquiterpenes from Nardostachys chinensis. , 1993, Chemical & pharmaceutical bulletin.

[20]  J. Waitz.  Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically , 1990 .

[21]  H. Mossmann,et al.  Direct skin test in highly sensitized guinea pigs for rapid and sensitive determination of staphylococcal enterotoxin B , 1983, Applied and environmental microbiology.