Sharka: The Past, The Present and The Future

Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

[1]  Frances S Ligler,et al.  Nanomaterials in analytical chemistry. , 2013, Analytical chemistry.

[2]  J. Diaz‐ruiz,et al.  Comparative analysis of transcriptomic and hormonal responses to compatible and incompatible plant-virus interactions that lead to cell death. , 2012, Molecular plant-microbe interactions : MPMI.

[3]  W. Miller,et al.  Interaction of the Trans-Frame Potyvirus Protein P3N-PIPO with Host Protein PCaP1 Facilitates Potyvirus Movement , 2012, PLoS pathogens.

[4]  R. De Francesco,et al.  Metabolism of Phosphatidylinositol 4-Kinase IIIα-Dependent PI4P Is Subverted by HCV and Is Targeted by a 4-Anilino Quinazoline with Antiviral Activity , 2012, PLoS pathogens.

[5]  M. Glasa,et al.  The 3′-proximal part of the Plum pox virus P1 gene determinates the symptom expression in two herbaceous host plants , 2012, Virus Genes.

[6]  D. Goss,et al.  Poly(A)-binding protein increases the binding affinity and kinetic rates of interaction of viral protein linked to genome with translation initiation factors eIFiso4F and eIFiso4F·4B complex. , 2012, Biochemistry.

[7]  S. Parveen,et al.  Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging. , 2012, Nanomedicine : nanotechnology, biology, and medicine.

[8]  F. Qu,et al.  Tritimovirus P1 functions as a suppressor of RNA silencing and an enhancer of disease symptoms. , 2012, Virus research.

[9]  S. Banerjee,et al.  Functional regulation of PVBV Nuclear Inclusion protein-a protease activity upon interaction with Viral Protein genome-linked and phosphorylation. , 2012, Virology.

[10]  M. Harris,et al.  Synthesis and application of virus‐based hybrid nanomaterials , 2012, Biotechnology and bioengineering.

[11]  R. Jain,et al.  Sequence Motifs Comparisons Establish a Functional Portrait of a Multifunctional Protein HC-Pro from Papaya Ringspot Potyvirus , 2008, Journal of Plant Biochemistry and Biotechnology.

[12]  S. German-Retana,et al.  The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E. , 2012, The Journal of general virology.

[13]  Guopeng Li,et al.  Synthesis of size-controlled Fe3O4@SiO2 magnetic nanoparticles for nucleic acid analysis. , 2012, Journal of nanoscience and nanotechnology.

[14]  V. Adam,et al.  Isolation of metallothionein from cells derived from aggressive form of high‐grade prostate carcinoma using paramagnetic antibody‐modified microbeads off‐line coupled with electrochemical and electrophoretic analysis , 2011, Electrophoresis.

[15]  R. Pires das Neves,et al.  Nanoparticles for intracellular-targeted drug delivery , 2011, Nanotechnology.

[16]  J. Hadersdorfer,et al.  Fast and reliable detection of Plum pox virus in woody host plants using the Blue LAMP protocol , 2011 .

[17]  I. Elmaghraby,et al.  SEROLOGICAL AND MOLECULAR CHARACTERIZATION OF ISOLATES OF PLUM POX VIRUS STRAIN EL AMAR TO BETTER UNDERSTAND ITS DIVERSITY, EVOLUTION, AND UNIQUE GEOGRAPHICAL DISTRIBUTION , 2011 .

[18]  W. Denny,et al.  Targeting of nanoparticles in cancer: drug delivery and diagnostics , 2011, Anti-cancer drugs.

[19]  S. Mitragotri,et al.  Adaptive micro and nanoparticles: temporal control over carrier properties to facilitate drug delivery. , 2011, Advanced drug delivery reviews.

[20]  N. Yoshida,et al.  Variability in the P1 gene helps to refine phylogenetic relationships among leek yellow stripe virus isolates from garlic , 2011, Archives of Virology.

[21]  L. Peña,et al.  Resistance to Plum pox virus in plants expressing cytosolic and nuclear single‐chain antibodies against the viral RNA NIb replicase , 2011 .

[22]  R.-H. Wen,et al.  Amino acid changes in P3, and not the overlapping pipo-encoded protein, determine virulence of soybean mosaic virus on functionally immune Rsv1-genotype soybean. , 2011, Molecular plant pathology.

[23]  J. Valkonen,et al.  Why do viruses need phloem for systemic invasion of plants? , 2011, Plant science : an international journal of experimental plant biology.

[24]  M Laird Forrest,et al.  Lymphatic drug delivery using engineered liposomes and solid lipid nanoparticles. , 2011, Advanced drug delivery reviews.

[25]  S. Dallot,et al.  First Report of the Plum Pox Virus Recombinant Strain on Peach in Bulgaria. , 2011, Plant disease.

[26]  H. Radecka,et al.  Impedimetric Immunosensor for Detection of Plum Pox Virus in Plant Extracts , 2011 .

[27]  S. Sells,et al.  Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen , 2011, Journal of Clinical Microbiology.

[28]  W. Turechek,et al.  Ecology and management of whitefly-transmitted viruses of vegetable crops in Florida. , 2011, Virus research.

[29]  Alexander M Seifalian,et al.  Fluorescence nanoparticles “quantum dots” as drug delivery system and their toxicity: a review , 2011, Journal of drug targeting.

[30]  T. Candresse,et al.  Sequence variability, recombination analysis, and specific detection of the W strain of Plum pox virus. , 2011, Phytopathology.

[31]  A. Piqueras,et al.  Implication of peroxidase activity in development of healthy and PPV-infected micropropagated GF305 peach plants , 2011, Plant Growth Regulation.

[32]  Li-Fang Wang,et al.  Design of magnetic nanoparticles-assisted drug delivery system. , 2011, Current pharmaceutical design.

[33]  K. Rantalainen,et al.  Potyviral VPg Enhances Viral RNA Translation and Inhibits Reporter mRNA Translation In Planta , 2011, Journal of Virology.

[34]  Jinzhong Lin,et al.  Structure of the Autocatalytic Cysteine Protease Domain of Potyvirus Helper-component Proteinase* , 2011, The Journal of Biological Chemistry.

[35]  Wolfgang Becker,et al.  Nanoparticles and microparticles for skin drug delivery. , 2011, Advanced drug delivery reviews.

[36]  K. Değirmenci,et al.  Update on Plum pox virus distribution in Turkey , 2011 .

[37]  M. Navrátil,et al.  P1 peptidase – a mysterious protein of family Potyviridae , 2011, Journal of Biosciences.

[38]  Nancy L. Richwine,et al.  Molecular, ultrastructural, and biological characterization of Pennsylvania isolates of Plum pox virus. , 2011, Phytopathology.

[39]  Carlos D. Garcia,et al.  Recent applications of carbon-based nanomaterials in analytical chemistry: critical review. , 2011, Analytica chimica acta.

[40]  D. Gramaje,et al.  Co‐operational PCR Coupled with Dot Blot Hybridization for the Detection of Phaeomoniella chlamydospora on Infected Grapevine Wood , 2011 .

[41]  P. Martínez-Gómez,et al.  Plum pox virus (sharka) sensitivity in Prunus salicina and Prunus cerasifera cultivars against a Dideron‐type isolate , 2011 .

[42]  Jianping Chen,et al.  Interaction between potyvirus P3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants , 2011, Virus Genes.

[43]  Shieh-Yueh Yang,et al.  Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay , 2011 .

[44]  A. Seifalian,et al.  Current trends in the application of nanoparticles in drug delivery. , 2011, Current medicinal chemistry.

[45]  S. Dallot,et al.  Mediterranean and central-eastern European countries host viruses of two different clades of plum pox virus strain M , 2011, Archives of Virology.

[46]  P. Martínez-Gómez,et al.  Changes in the antioxidative metabolism induced by Apple chlorotic leaf spot virus infection in peach [Prunus persica (L.) Batsch] , 2011 .

[47]  K. Tomizawa,et al.  Nanoparticle-based Drug Delivery Systems for Solid Brain Tumors , 2011 .

[48]  X. Su,et al.  Magnetic Nanoparticles in Brain Disease Diagnosis and Targeting Drug Delivery , 2011 .

[49]  Christine Shea,et al.  Nanotechnology as general-purpose technology: empirical evidence and implications , 2011, Technol. Anal. Strateg. Manag..

[50]  S. Franzen,et al.  The Red clover necrotic mosaic virus capsid as a multifunctional cell targeting plant viral nanoparticle. , 2011, Bioconjugate chemistry.

[51]  E. Peña,et al.  Resistance to Citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-RNA silencing. , 2011, Journal of biotechnology.

[52]  Yvonne Lokko,et al.  Nanotechnology and synthetic biology - Potential in crop improvement , 2011 .

[53]  F. Palmisano,et al.  INCIDENCE OF PLUM POX VIRUS AND ITS STRAINS IN KOSOVO , 2011 .

[54]  Aiming Wang,et al.  Mutations in the P3 protein of Soybean mosaic virus G2 isolates determine virulence on Rsv4-genotype soybean. , 2011, Molecular plant-microbe interactions : MPMI.

[55]  A. Bandyopadhyay,et al.  Rapid isolation of genomic DNA from E. coli XL1 Blue strain approaching bare magnetic nanoparticles , 2011 .

[56]  A. Soitamo,et al.  HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers , 2011, BMC Plant Biology.

[57]  Liying Sun,et al.  Mapping the self-interacting domains of TuMV HC-Pro and the subcellular localization of the protein , 2011, Virus Genes.

[58]  P. Tompa,et al.  Structural Flexibility Allows the Functional Diversity of Potyvirus Genome-Linked Protein VPg , 2010, Journal of Virology.

[59]  W. Song,et al.  The Nuclear Inclusion a (NIa) Protease of Turnip Mosaic Virus (TuMV) Cleaves Amyloid-β , 2010, PloS one.

[60]  A. Zherdev,et al.  Interaction of Plum Pox Virus with Specific Colloidal Gold-Labeled Antibodies and Development of Immunochromatographic Assay of the Virus , 2010, Biochemistry (Moscow).

[61]  T. Natsuaki,et al.  Soybean leaf rugose mosaic virus, a new soilborne virus in the family Potyviridae, isolated from soybean in Japan , 2010, Journal of General Plant Pathology.

[62]  Florence Sanchez,et al.  Nanotechnology in concrete – A review , 2010 .

[63]  A. López-Quílez,et al.  Detection, characterization, epidemiology and eradication of Plum pox virus Marcus type in Spain. , 2010 .

[64]  T. Nann,et al.  Use of Nanoparticles to Study and Manipulate Plant cells , 2010 .

[65]  Yelena Katsenovich,et al.  Nanomedicine: magnetic nanoparticles and their biomedical applications. , 2010, Current medicinal chemistry.

[66]  A. Gibbs,et al.  Potyviruses and the digital revolution. , 2010, Annual review of phytopathology.

[67]  B. Ratna,et al.  Virus hybrids as nanomaterials for biotechnology. , 2010, Current opinion in biotechnology.

[68]  V. Adam,et al.  Automated pipetting system coupled with micro- andnanoparticles as a new tool for study of nucleic acid , 2010 .

[69]  Yong-Hwan Lee Erratum : Development of Recombinant Coat Protein Antibody Based IC-RT-PCR and Comparison of Its Sensitivity with Other Immunoassays for the Detection of Papaya ringspot virus Isolates from India , 2010 .

[70]  K. Kasschau,et al.  Formation of Complexes at Plasmodesmata for Potyvirus Intercellular Movement Is Mediated by the Viral Protein P3N-PIPO , 2010, PLoS pathogens.

[71]  A. Zherdev,et al.  Correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity. , 2010, Journal of immunological methods.

[72]  W. Shen,et al.  Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulin. , 2010, Molecular plant pathology.

[73]  R.-H. Wen,et al.  Mutational analysis of the putative pipo of soybean mosaic virus suggests disruption of PIPO protein impedes movement. , 2010, Virology.

[74]  H. Hamamoto,et al.  First report of plum pox virus infecting Japanese apricot (Prunus mume Sieb. et Zucc.) in Japan , 2010, Journal of General Plant Pathology.

[75]  M. Sreenivasulu,et al.  Development of Recombinant Coat Protein Antibody Based IC-RT-PCR and Comparison of its Sensitivity with Other Immunoassays for the Detection of Papaya Ringspot Virus Isolates from India , 2010 .

[76]  V. Adam,et al.  Easy to use and rapid isolation and detection of a viral nucleic acid by using paramagnetic microparticles and carbon nanotubes-based screen-printed electrodes , 2010 .

[77]  S. Elena,et al.  Compensatory molecular evolution of HC-Pro, an RNA-silencing suppressor from a plant RNA virus. , 2010, Molecular biology and evolution.

[78]  C. Masuta,et al.  HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants , 2010, Virus Genes.

[79]  Aiming Wang,et al.  The Tobacco etch virus P3 protein forms mobile inclusions via the early secretory pathway and traffics along actin microfilaments. , 2010, Virology.

[80]  U. Sonnewald,et al.  HSP70 and Its Cochaperone CPIP Promote Potyvirus Infection in Nicotiana benthamiana by Regulating Viral Coat Protein Functions , 2010, Plant Cell.

[81]  D. Pamfil,et al.  Genetic diversity of Plum pox virus isolates in Muntenia, Romania. , 2010 .

[82]  3. Arges,et al.  Genetic diversity of Plum pox virus Isolates in Muntenia , Romania , 2010 .

[83]  N. Chua,et al.  Discriminating mutations of HC-Pro of zucchini yellow mosaic virus with differential effects on small RNA pathways involved in viral pathogenicity and symptom development. , 2010, Molecular plant-microbe interactions : MPMI.

[84]  J. P. Thompson,et al.  Detection of Polymyxa graminis in a barley crop in Australia , 2010, Australasian Plant Pathology.

[85]  D. Otzen,et al.  Interaction of a potyviral VPg with anionic phospholipid vesicles. , 2009, Virology.

[86]  J. Dale,et al.  Expression of Potato virus Y cytoplasmic inclusion protein in tobacco results in disorganization of parenchyma cells, distortion of epidermal cells, and induces mitochondrial and chloroplast abnormalities, formation of membrane whorls and atypical lipid accumulation. , 2009, Micron.

[87]  Joydeep Dutta,et al.  Nanotechnology applications in pollution sensing and degradation in agriculture: a review , 2009 .

[88]  V. Adam,et al.  Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection. , 2009, Talanta.

[89]  A. C. Puhl,et al.  Covalent immobilization of tobacco‐etch‐virus NIa protease: a useful tool for cleavage of the histidine tag of recombinant proteins , 2009, Biotechnology and applied biochemistry.

[90]  S. Shrivastava,et al.  INVITED PAPER: Agrifood Nanotechnology: A Tiny Revolution in Food and Agriculture , 2009 .

[91]  M. J. Adams,et al.  Protein-protein interactions in two potyviruses using the yeast two-hybrid system. , 2009, Virus research.

[92]  T. Candresse,et al.  Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in orchards in the Ankara province of Turkey. , 2009, Virus research.

[93]  D. James,et al.  First Report of Plum pox virus Recombinant Strain on Prunus spp. in Canada. , 2009, Plant disease.

[94]  Minmin Liang,et al.  Application of nanomaterials in environmental analysis and monitoring. , 2009, Journal of nanoscience and nanotechnology.

[95]  M. Cambra,et al.  Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR. , 2009, International microbiology : the official journal of the Spanish Society for Microbiology.

[96]  D. Rochon Fungal Transmission of Plant Viruses , 2009, Current protocols in microbiology.

[97]  A. Maule,et al.  The Cytosolic Protein Response as a Subcomponent of the Wider Heat Shock Response in Arabidopsis[W] , 2009, The Plant Cell Online.

[98]  S. Bai,et al.  Fabrication of mono-sized magnetic anion exchange beads for plasmid DNA purification. , 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[99]  K. Knauer,et al.  Nano-materials: research needs in agriculture. , 2009 .

[100]  K. Knauer,et al.  Nano-materials - the need for research in agriculture. , 2009 .

[101]  N. Milosevic,et al.  Plum pox virus as a stress factor in the one-year-old shoot and fruit growth and yield of plum cv. Stanley. , 2009 .

[102]  D. Pamfil,et al.  Serological and Molecular Typing of Plum pox Virus Isolates in the Transylvania, Romania , 2009 .

[103]  S. German-Retana,et al.  Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus. , 2009, Molecular plant pathology.

[104]  D. Šafářová,et al.  Differentiation of Plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer PCR analysis of HC-Pro genome region. , 2009, Acta virologica.

[105]  N. Milosevic,et al.  Plum pox virus as a stress factor in the vegetative growth, fruit growth and yield of plum (Prunus domestica) cv. ‘Cacanska Rodna’ , 2009, European Journal of Plant Pathology.

[106]  M. Young,et al.  A library of protein cage architectures as nanomaterials. , 2009, Current topics in microbiology and immunology.

[107]  M. Hema,et al.  Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles. , 2008, Indian journal of experimental biology.

[108]  P. Martínez-Gómez,et al.  Study of long-distance movement of Plum pox virus (Sharka) as an alternative resistance-evaluation method in Prunus , 2008 .

[109]  S. Behrens Synthesis of inorganic nanomaterials mediated by protein assemblies , 2008 .

[110]  C. Kao,et al.  Self-assembly approaches to nanomaterial encapsulation in viral protein cages. , 2008, Journal of materials chemistry.

[111]  Trevor Douglas,et al.  Plant viruses as biotemplates for materials and their use in nanotechnology. , 2008, Annual review of phytopathology.

[112]  J. Irudayaraj,et al.  Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA. , 2008, Analytical biochemistry.

[113]  Kuan-Chun Chen,et al.  A single amino acid of niapro of papaya ringspot virus determines host specificity for infection of papaya. , 2008, Molecular plant-microbe interactions : MPMI.

[114]  A. Gibbs,et al.  The potyviruses of Australia , 2008, Archives of Virology.

[115]  M. Krajacic,et al.  MOLECULAR TYPING OF PLUM POX VIRUS ISOLATES IN CROATIA , 2008 .

[116]  D. Pamfil,et al.  SEROLOGICAL AND MOLECULAR TYPING OF PLUM POX VIRUS ISOLATES IN THE NORTH OF ROMANIA , 2008 .

[117]  I. Kamenova PRUNUS CERASIFERA AS A HOST OF PLUM POX VIRUS IN BULGARIA , 2008 .

[118]  V. Bozhkova,et al.  EXPERIMENTS ON TRANSMISSION OF PLUM POX VIRUS THROUGH PRUNUS SEEDS , 2008 .

[119]  E. Kiss,et al.  ORNAMENTAL PRUNUS SPECIES AS NEW NATURAL HOSTS OF PLUM POX VIRUS AND THEIR IMPORTANCE IN THE SPREAD OF THE VIRUS IN HUNGARY , 2008 .

[120]  E. Olmos,et al.  Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus , 2008, Journal of experimental botany.

[121]  Andrew E Firth,et al.  An overlapping essential gene in the Potyviridae , 2008, Proceedings of the National Academy of Sciences.

[122]  W.-S. Kim,et al.  Direct real-time PCR detection of Plum pox virus in field surveys in Ontario , 2008 .

[123]  F. Valentini,et al.  Nanomaterials and Analytical Chemistry , 2008 .

[124]  C. Leyronas,et al.  ROLE OF FUNGAL ASCOSPORES IN THE INFECTION OF ORCHARDGRASS (DACTYLIS GLOMERATA) BY EPICHLOË TYPHINA AGENT OF CHOKE DISEASE , 2008 .

[125]  M. Scortichini,et al.  FURTHER MOLECULAR CHARACTERIZATION OF PSEUDOMONAS SYRINGAE pv. CORYLI , 2008 .

[126]  Ç. U. Serçe,et al.  SEQUENCE VARIABILITY OF HOP STUNT VIROID ISOLATES FROM STONE FRUITS IN TURKEY , 2008 .

[127]  E. Yángüez,et al.  Host-specific effect of P1 exchange between two potyviruses. , 2008, Molecular plant pathology.

[128]  D. Šafářová,et al.  Genetic variability of Plum pox virus isolates in the Czech Republic , 2008, European Journal of Plant Pathology.

[129]  P. Kofinas,et al.  Self-assembly of virus-structured high surface area nanomaterials and their application as battery electrodes. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[130]  N. Vassilakos,et al.  PRELIMINARY BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF MILD PPV ISOLATES NATURALLY INDUCED IN N. BENTHAMIANA PLANTS , 2008 .

[131]  S. Dallot,et al.  SPATIAL PATTERN AND GENETIC STRUCTURE OF PPV-M IN A DELIMITED AREA OF STONE FRUIT ORCHARDS IN SOUTHERN FRANCE , 2008 .

[132]  M. Neumüller,et al.  THE HYPERSENSITIVITY OF EUROPEAN PLUM (PRUNUS DOMESTICA L.) AGAINST THE PLUM POX VIRUS , 2008 .

[133]  M. Cambra,et al.  PROPOSAL FOR A NEW INTERNATIONAL PROTOCOL FOR DETECTION AND IDENTIFICATION OF PLUM POX VIRUS: VALIDATION OF THE TECHNIQUES , 2008 .

[134]  M. Cambra,et al.  Isothermal amplification for detection of Plum pox virus , 2008 .

[135]  M. Monsion,et al.  COMPARATIVE TECHNIQUES TO PERFORM KOCH'S POSTULATES WITH PLUM POX VIRUS , 2008 .

[136]  M. Cambra,et al.  Spot real-time RT-PCR a method for direct detection of Plum pox virus avoiding RNA extraction , 2008 .

[137]  D. Šafářová,et al.  The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic , 2008 .

[138]  S. Dallot,et al.  PREVALENCE AND GENETIC STRUCTURE OF PPV-M IN SIX EUROPEAN COUNTRIES , 2008 .

[139]  J. Polák,et al.  THE DETERMINATION OF SOURCES OF RESISTANCE TO PLUM POX VIRUS SUITABLE FOR PEACH , 2008 .

[140]  M. Călinescu,et al.  DETECTION OF THE VIRAL DISEASES PRESENTLY WITH THE STONE FRUIT SPECIES IN ROMANIA , 2008 .

[141]  H. Ilbağı,et al.  Prunus spinosa L. - A natural wild host of some important fruit viruses in Tekirdağ, Turkey , 2008 .

[142]  A. Palloix,et al.  Natural variation and functional analyses provide evidence for co-evolution between plant eIF4E and potyviral VPg. , 2008, The Plant journal : for cell and molecular biology.

[143]  A. Fereres,et al.  Comparison ofPotato Virus Y andPlum Pox Virus transmission by two aphid species in relation to their probing behavior , 2006, Phytoparasitica.

[144]  I. O. Elibuyuk Current situation of sharka disease in Ankara, Turkey , 2004, Phytoparasitica.

[145]  J. García,et al.  Identification of Plum pox virus pathogenicity determinants in herbaceous and woody hosts. , 2008, Molecular plant-microbe interactions : MPMI.

[146]  M. Glasa,et al.  Plum pox virus variability detected by the advanced analytical methods. , 2008, Acta virologica.

[147]  V. Polischuk,et al.  UDK 634.22:632.38 MOLECULAR IDENTIFICATION OF PLUM POX VIRUS ISOLATES FROM LITHUANIA AND UKRAINE , 2008 .

[148]  M. Ghanim,et al.  Oligonucleotide microarray-based detection and genotyping of Plum pox virus. , 2008, Journal of virological methods.

[149]  Aiming Wang,et al.  Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection , 2008, BMC Genomics.

[150]  G. Reighard,et al.  Origin of resistance to plum pox virus in Apricot: what new AFLP and targeted SSR data analyses tell , 2008, Tree Genetics & Genomes.

[151]  T. Perring,et al.  Intraspecific Variation in Zucchini Yellow Mosaic Virus Transmission by Myzus persicae and the Impact of Aphid Host Plant , 2007, Journal of economic entomology.

[152]  M. J. Adams,et al.  A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host. , 2007, Molecular plant pathology.

[153]  P. Clement,et al.  First Report of Plum pox virus on Plum in New York State. , 2007, Plant disease.

[154]  M. Sánchez-Pina,et al.  Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds. , 2007, Physiologia plantarum.

[155]  N. Waipara,et al.  Partial sequencing of the genomic RNA of Araujia mosaic virus and comparison of the coat protein sequence with those of other potyviruses , 2007, Archives of Virology.

[156]  Z. Kiraly,et al.  Plant resistance to pathogen infection: Forms and mechanisms of innate and acquired resistance , 2007 .

[157]  M. Cambra,et al.  Co‐operational PCR coupled with dot blot hybridization for detection and 16SrX grouping of phytoplasmas , 2007 .

[158]  B. Manachini,et al.  Role of Myzus persicae (Hemiptera: Aphididae) and Its Secondary Hosts in Plum Pox Virus Propagation , 2007, Journal of economic entomology.

[159]  R. Srinivasan,et al.  Effect of Mixed Viral Infections (Potato Virus Y–Potato Leafroll Virus) on Biology and Preference of VectorsMyzus persicae andMacrosiphum euphorbiae (Hemiptera: Aphididae) , 2007, Journal of economic entomology.

[160]  J. Chen,et al.  The ‘6K1’ protein of a strain of Soybean mosaic virus localizes to the cell periphery , 2007, Archives of Virology.

[161]  M. Ravelonandro,et al.  Susceptibility of ornamental Prunus to plum pox potyvirus infection , 2007 .

[162]  O. Le Gall,et al.  Central domain of a potyvirus VPg is involved in the interaction with the host translation initiation factor eIF4E and the viral protein HcPro. , 2007, The Journal of general virology.

[163]  J. López-Moya,et al.  Recombination and gene duplication in the evolutionary diversification of P1 proteins in the family Potyviridae. , 2007, The Journal of general virology.

[164]  R. Scorza,et al.  Prunus Host Range of Plum pox virus (PPV) in the United States by Aphid and Graft Inoculation. , 2007, Plant disease.

[165]  S. Adkins,et al.  Identification and characterization of a novel whitefly-transmitted member of the family potyviridae isolated from cucurbits in Florida. , 2007, Phytopathology.

[166]  M. Navrátil,et al.  EVALUATION OF PPV SYMPTOMS ON PLUM FRUITS , 2007 .

[167]  Wen-Chi Hu,et al.  Zantedeschia mild mosaic virus, a new widespread virus in calla lily, detected by ELISA, dot‐blot hybridization and IC‐RT‐PCR , 2007 .

[168]  Q. Wan,et al.  Surface Modification of Magnetic Silica Microspheres and Its Application to the Isolation of Plant Genomic Nucleic Acids , 2007 .

[169]  T. Candresse,et al.  First Report of the Presence of Plum pox virus Rec Strain in Turkey. , 2007, Plant disease.

[170]  M. Cambra,et al.  Isothermal amplification coupled with rapid flow-through hybridisation for sensitive diagnosis of Plum pox virus. , 2007, Journal of virological methods.

[171]  Y. Yap,et al.  C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum , 2007, Virus Genes.

[172]  I. Moeller,et al.  Eriophyid mite transmission and host range of a Brome streak mosaic virus isolate derived from a full-length cDNA clone , 2007, Archives of Virology.

[173]  M. Krajacic,et al.  Occurrence and distribution of Plum pox virus in Croatia , 2008 .

[174]  C. Hjulsager,et al.  Multiple determinants in the coding region of Pea seed-borne mosaic virus P3 are involved in virulence against sbm-2 resistance. , 2006, Virology.

[175]  J. Laliberté,et al.  Visualization of the Interaction between the Precursors of VPg, the Viral Protein Linked to the Genome of Turnip Mosaic Virus, and the Translation Eukaryotic Initiation Factor iso 4E In Planta , 2006, Journal of Virology.

[176]  P. Periago,et al.  The apoplastic antioxidant system in Prunus : response to long-term plum pox virus infection , 2006 .

[177]  C. Chiang,et al.  Application of silica–magnetite nanocomposites to the isolation of ultrapure plasmid DNA from bacterial cells , 2006 .

[178]  P. Periago,et al.  Effect of Plum Pox virus infection on the antioxidant capacity of leaf apoplast from apricot plants , 2006 .

[179]  J. Polák Hosts and symptoms of Plum pox virus: woody species other than fruit and ornamental species of Prunus , 2006 .

[180]  I. Križanac,et al.  Plum pox virus (PPV) in Croatia , 2006 .

[181]  Y. Prichodko Plum pox virus (PPV) in Russia , 2006 .

[182]  T. Malinowski Plum pox virus (PPV) in Poland , 2006 .

[183]  D. Boscia,et al.  Plum pox virus (PPV) in Italy , 2006 .

[184]  F. Ismaeil Plum pox virus (PPV) in Syria , 2006 .

[185]  J. Verhoeven,et al.  Plum pox virus (PPV) in the Netherlands , 2006 .

[186]  M. Collao,et al.  Plum pox virus (PPV) in Chile , 2006 .

[187]  M. Kölber Plum pox virus (PPV) in Hungary , 2006 .

[188]  C. Varveri Plum pox virus (PPV) in Greece , 2006 .

[189]  D. Ducasse,et al.  Plum pox virus (PPV) in Argentina , 2006 .

[190]  P. Speich Plum pox virus (PPV) in France , 2006 .

[191]  K. Çağlayan Plum pox virus (PPV) in Turkey , 2006 .

[192]  N. Capote,et al.  Plum pox virus (PPV) in Pakistan , 2006 .

[193]  M. Ravelonandro,et al.  Plum pox virus (PPV) in Tunisia , 2006 .

[194]  D. James,et al.  Hosts and symptoms of Plum pox virus: ornamental and wild Prunus species , 2006 .

[195]  A. Lemmetty Plum pox virus (PPV) in Finland , 2006 .

[196]  R. Mumford Plum pox virus (PPV) in the United Kingdom , 2006 .

[197]  D. Jevremović,et al.  Plum pox virus (PPV) in Serbia , 2006 .

[198]  N. Capote,et al.  Plum pox virus (PPV) in Kazakhstan , 2006 .

[199]  W. Jarausch Plum pox virus (PPV) in Germany , 2006 .

[200]  I. Kamenova,et al.  Plum pox virus (PPV) in Bulgaria , 2006 .

[201]  E. Choueiri Plum pox virus (PPV) in Lebanon , 2006 .

[202]  L. Levy Plum pox virus (PPV) in the United States of America , 2006 .

[203]  J. García,et al.  Causal agent of sharka disease: Plum pox virus genome and function of gene products , 2006 .

[204]  M. V. Marn,et al.  Plum pox virus (PPV) in Slovenia , 2006 .

[205]  P. Kondratenko,et al.  Plum pox virus (PPV) in Ukraine , 2006 .

[206]  A. Chernets,et al.  Plum pox virus (PPV) in Moldova , 2006 .

[207]  A. Myrta,et al.  Plum pox virus (PPV) in Albania , 2006 .

[208]  I. Zagrai,et al.  Plum pox virus (PPV) in Romania , 2006 .

[209]  R. Khakvar,et al.  Plum pox virus (PPV) in Iran , 2006 .

[210]  J. Staniulis Plum pox virus (PPV) in Lithuania , 2006 .

[211]  M. Glasa Plum pox virus (PPV) in Slovakia , 2006 .

[212]  A. Cano,et al.  Plum pox virus (PPV) in Spain , 2006 .

[213]  S. Matić,et al.  Plum pox virus (PPV) in Bosnia and Herzegovina , 2006 .

[214]  S. Youssef,et al.  Plum pox virus (PPV) in Egypt , 2006 .

[215]  M. Navrátil Plum pox virus (PPV) in the Czech Republic , 2006 .

[216]  P. Gugerli,et al.  Plum pox virus (PPV) in Switzerland , 2006 .

[217]  D. Šafářová,et al.  Plum pox virus (PPV) in China , 2006 .

[218]  D. Blystad,et al.  Plum pox virus (PPV) in Norway , 2006 .

[219]  E. Maiss,et al.  Detection of 6K1 as a mature protein of 6 kDa in plum pox virus-infected Nicotiana benthamiana. , 2006, The Journal of general virology.

[220]  W. Miller,et al.  Cap-independent translation of plant viral RNAs. , 2006, Virus research.

[221]  J. Chroboczek,et al.  Potyvirus terminal protein VPg, effector of host eukaryotic initiation factor eIF4E. , 2006, Biochimie.

[222]  O. Le Gall,et al.  Plum pox virus induces differential gene expression in the partially resistant stone fruit tree Prunus armeniaca cv. Goldrich. , 2006, Gene.

[223]  A. Svircev,et al.  Plum pox virus in Canada: progress in research and future prospects for disease control , 2006 .

[224]  Yong Zhou Recent Progress in Biomolecule-Templated Nanomaterials , 2006 .

[225]  A. Palloix,et al.  Different mutations in the genome-linked protein VPg of potato virus Y confer virulence on the pvr2(3) resistance in pepper. , 2006, Molecular plant-microbe interactions : MPMI.

[226]  D. Ducasse,et al.  First Report in Argentina of Plum pox virus Causing Sharka Disease in Prunus. , 2006, Plant disease.

[227]  J. Theilmann,et al.  Sequence analysis of isolates of the Canadian Plum pox virus, and comparisons to isolates from Europe and the United States , 2006 .

[228]  S. German-Retana,et al.  The potyviral virus genome‐linked protein VPg forms a ternary complex with the eukaryotic initiation factors eIF4E and eIF4G and reduces eIF4E affinity for a mRNA cap analogue , 2006, The FEBS journal.

[229]  J. García,et al.  Identification of a plum pox virus CI-interacting protein from chloroplast that has a negative effect in virus infection. , 2006, Molecular plant-microbe interactions : MPMI.

[230]  M. Cambra,et al.  Interference Between D and M Types of Plum pox virus in Japanese Plum Assessed by Specific Monoclonal Antibodies and Quantitative Real-Time Reverse Transcription-Polymerase Chain Reaction. , 2006, Phytopathology.

[231]  D. James,et al.  Real-time RT-PCR and SYBR Green I melting curve analysis for the identification of Plum pox virus strains C, EA, and W: effect of amplicon size, melt rate, and dye translocation. , 2006, Journal of virological methods.

[232]  P. Martínez-Gómez,et al.  Behaviour of six Prunus rootstocks in presence of sharka ( Plum Pox Potyvirus) , 2006 .

[233]  F. Tab,et al.  LOCATION OF PLUM POX VIRUS IN PEACH AND APRICOT TREES , 2006 .

[234]  S. Matić,et al.  Diversity of Plum pox virus isolates in Bosnia and Herzegovina , 2006 .

[235]  E. Olmos,et al.  Long-term plum pox virus infection produces an oxidative stress in a susceptible apricot, Prunus armeniaca, cultivar but not in a resistant cultivar , 2006 .

[236]  D. Pamfil,et al.  FIRST DETECTION AND MOLECULAR CARACTERIZATION OF PLUM POX VIRUS RECOMBINANT STRAIN IN ROMANIA , 2006 .

[237]  D. James,et al.  The complete genome sequence of an El Amar isolate of plum pox virus (PPV) and its phylogenetic relationship to other PPV strains , 2006, Archives of Virology.

[238]  M. Cambra,et al.  Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids. , 2005, Journal of virological methods.

[239]  L. Stobbs,et al.  Distribution of Plum pox virus in Residential Sites, Commercial Nurseries, and Native Plant Species in the Niagara Region, Ontario, Canada. , 2005, Plant disease.

[240]  L. Chen,et al.  A comparative survey, by expressed sequence tag analysis, of genes expressed in peach leaves infected with Plum pox virus (PPV) and free from PPV , 2005 .

[241]  V. Savino,et al.  PRESENCE OF A RECOMBINANT ISOLATE OF PLUM POX VIRUS IN APULIA , 2005 .

[242]  F. Beaudoin,et al.  Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. , 2005, Molecular plant pathology.

[243]  D. Stenger,et al.  Plant Virus HC-Pro Is a Determinant of Eriophyid Mite Transmission , 2005, Journal of Virology.

[244]  C. Jianping Progress and prospects of studies on Polymyxa graminis and its transmitted cereal viruses in China , 2005 .

[245]  D. James,et al.  Nucleotide sequence analysis of Plum pox virus isolate W3174: evidence of a new strain. , 2005, Virus research.

[246]  T. Candresse,et al.  Analysis of recombinant Plum pox virus (PPV) isolates from Serbia confirms genetic homogeneity and supports a regional origin for the PPV-Rec subgroup , 2005, Archives of Virology.

[247]  A. Maule,et al.  Virus Induction of Heat Shock Protein 70 Reflects a General Response to Protein Accumulation in the Plant Cytosol1 , 2005, Plant Physiology.

[248]  D. Šafářová,et al.  First Incidence of Plum Pox Virus on Apricot Trees in China. , 2005, Plant disease.

[249]  P. Martínez-Gómez,et al.  Evaluation of resistance to sharka (Plum pox virus) of several Prunus rootstocks , 2005 .

[250]  D. James,et al.  Detection and differentiation of Plum pox virus using real-time multiplex PCR with SYBR Green and melting curve analysis: a rapid method for strain typing. , 2005, Journal of virological methods.

[251]  M. Ronjat,et al.  Effect of gadolinium on the ryanodine receptor/sarcoplasmic reticulum calcium release channel of skeletal muscle , 2005, The FEBS journal.

[252]  P. Rabinowicz,et al.  Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV) , 2005, Archives of Virology.

[253]  ChenJianping Progress and prospects of studies on Polymyxa graminis and its transmitted cereal viruses in China , 2005 .

[254]  E. Cortés,et al.  Production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of Mediterranean isolates , 2005, Archives of Virology.

[255]  M. Ravelonandro,et al.  Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants , 2005, Archives of Virology.

[256]  A. Dombrovsky,et al.  Aphid transmission of a potyvirus depends on suitability of the helper component and the N terminus of the coat protein , 2005, Archives of Virology.

[257]  P. Casati,et al.  Transmission of PPV‐M to Prunus persica by Brachycaudus schwartzi and Phorodon humuli (Hem., Aphididae) , 2004 .

[258]  P. Puustinen,et al.  Uridylylation of the Potyvirus VPg by Viral Replicase NIb Correlates with the Nucleotide Binding Capacity of VPg* , 2004, Journal of Biological Chemistry.

[259]  F. Gildow,et al.  Potential Prunus host range of PPV-PENN isolates by aphid transmission , 2004 .

[260]  G. Krczal,et al.  FIRST DETECTION AND MOLECULAR CHARACTERISATION OF PPV-M STRAINS IN PLUM ORCHARDS IN SOUTH-WESTERN GERMANY , 2004 .

[261]  J. Peyre,et al.  SPATIALLY ARRANGED DIVERSITY OF PLUM POX VIRUS (PPV) ISOLATES IN A CLUSTER OF ORCHARDS IN SOUTHERN FRANCE , 2004 .

[262]  S. Comes,et al.  EVALUATION OF CHERRY CULTIVARS FOR THEIR RESPONSE TO INFECTION BY PLUM POX VIRUS SWEET CHERRY STRAIN , 2004 .

[263]  M. Monsion,et al.  STRATEGY TO IDENTIFY AND TO CHARACTERIZE NEW ISOLATES OF PLUM POX VIRUS , 2004 .

[264]  G. Labonne,et al.  RISK ASSESSMENT OF CONTAMINATION OF CHERRY TREES BY PLUM POX VIRUS IN FRANCE , 2004 .

[265]  M. Glasa,et al.  Aphid transmission of natural recombinant plum pox virus isolates to different Prunus spp.-a contribution for understanding the epidemiology of an atypical PPV , 2004 .

[266]  F. Gildow,et al.  TRANSMISSION OF THREE NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS , 2004 .

[267]  P. Martínez-Gómez,et al.  Localization of coat protein and nucleic acid of Plum Pox Virus in Prunus Petiole and stem tissues , 2004 .

[268]  P. Martínez-Gómez,et al.  Utilization of almond as source of Plum Pox Virus resistance in peach breeding , 2004 .

[269]  E. Olmos,et al.  Effect of Plum Pox Virus infection on photosynthesis and antioxidant enzyme activity in peach 'GF305' , 2004 .

[270]  M. Cambra,et al.  EPIDEMIOLOGY OF PLUM POX VIRUS IN JAPANESE PLUMS IN SPAIN , 2004 .

[271]  I. Mavrič,et al.  CURRENT STATUS OF SHARKA DISEASE IN SLOVENIA , 2004 .

[272]  S. Spiegel,et al.  Detection and Partial Molecular Characterization of Two Plum pox virus Isolates from Plum and Wild Apricot in Southeast Kazakhstan. , 2004, Plant disease.

[273]  R. Frederick,et al.  Specific detection and quantification of Plum pox virus by real-time fluorescent reverse transcription-PCR. , 2004, Journal of virological methods.

[274]  H. Savithri,et al.  Potyviral NIa Proteinase, a Proteinase with Novel Deoxyribonuclease Activity* , 2004, Journal of Biological Chemistry.

[275]  Tomoko Watanabe,et al.  An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein. , 2004, The Journal of general virology.

[276]  J. Valkonen,et al.  Potyviral 6K2 protein long-distance movement and symptom-induction functions are independent and host-specific. , 2004, Molecular plant-microbe interactions : MPMI.

[277]  M. J. Adams,et al.  Investigating resistance to Barley mild mosaic virus , 2004 .

[278]  J. Laliberté,et al.  Turnip mosaic virus VPg does not disrupt the translation initiation complex but interferes with cap binding , 2004 .

[279]  J. Laliberté,et al.  Interaction of VPg-Pro of turnip mosaic virus with the translation initiation factor 4E and the poly(A)-binding protein in planta. , 2004, The Journal of general virology.

[280]  P. Lotrakul,et al.  Whitefly transmission of sweet potato viruses. , 2004, Virus research.

[281]  A. Maule,et al.  A Cysteine-Rich Plant Protein Potentiates Potyvirus Movement through an Interaction with the Virus Genome-Linked Protein VPg , 2004, Journal of Virology.

[282]  N. Mitter,et al.  Peanut Stripe Potyvirus Resistance in Peanut (Arachis Hypogaea L.) Plants Carrying Viral Coat Protein Gene Sequences , 2004, Transgenic Research.

[283]  L. Talbert,et al.  Resistance to wheat streak mosaic virus in transgenic wheat expressing the viral replicase (NIb) gene , 2000, Molecular Breeding.

[284]  M. Fuchs,et al.  Epidemiology of an aphid nontransmissible potyvirus in fields of nontransgenic and coat protein transgenic squash , 1999, Transgenic Research.

[285]  M. T. Martín,et al.  Non-structural plum pox potyvirus proteins detected by immunogold labelling , 1997, European Journal of Plant Pathology.

[286]  Y. Chupeau,et al.  Coat protein gene-mediated protection in Lactuca sativa against lettuce mosaic potyvirus strains , 1997, Molecular Breeding.

[287]  M. Glasa,et al.  A simplified RT-PCR-based detection of recombinant Plum pox virus isolates. , 2004, Acta virologica.

[288]  M. Meyer,et al.  Identification of structural similarities between putative transmission proteins of Polymyxa and Spongospora transmitted bymoviruses and furoviruses , 2004, Virus Genes.

[289]  P. Bianco,et al.  Detection and characterization of Plum Pox Virus isolates in Lombardia and Veneto regions (Italy) , 2004 .

[290]  C. Fauquet,et al.  Structural characterization of Tobacco etch virus coat protein mutants , 2004, Archives of Virology.

[291]  E. Maiss,et al.  The complete nucleotide sequence of Plum pox virus isolates from sweet (PPV-SwC) and sour (PPV-SoC) cherry and their taxonomic relationships within the species , 2003, Archives of Virology.

[292]  K. Çağlayan,et al.  Detection and Characterization of Plum Pox Potyvirus (PPV) by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey , 2003 .

[293]  P. Martínez-Gómez,et al.  Resistance of almond cultivars to Plum pox virus (sharka) , 2003 .

[294]  K. Nakahara,et al.  The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg-HCPro and VPg-VPg interactions. , 2003, The Journal of general virology.

[295]  D. James,et al.  Detection of a New and Unusual Isolate of Plum pox virus in Plum (Prunus domestica). , 2003, Plant disease.

[296]  C. Jenner,et al.  The dual role of the potyvirus P3 protein of Turnip mosaic virus as a symptom and avirulence determinant in brassicas. , 2003, Molecular plant-microbe interactions : MPMI.

[297]  F. Dosba PROGRESS AND PROSPECTS IN STONE FRUIT BREEDING , 2003 .

[298]  C. Jenner,et al.  Strains of Turnip mosaic potyvirus as defined by the molecular analysis of the coat protein gene of the virus. , 2003, Virus research.

[299]  S. German-Retana,et al.  Structural Characterization of HC-Pro, a Plant Virus Multifunctional Protein* , 2003, Journal of Biological Chemistry.

[300]  C. Upton,et al.  Partial molecular characterization of plum pox virus isolates occurring in Canada , 2003 .

[301]  C. Fraser,et al.  The genome‐linked protein VPg of the Norwalk virus binds eIF3, suggesting its role in translation initiation complex recruitment , 2003, The EMBO journal.

[302]  M. Hema,et al.  Development of recombinant coat protein antibody based IC-RT-PCR for detection and discrimination of sugarcane streak mosaic virus isolates from Southern India , 2003, Archives of Virology.

[303]  T. Thomidis,et al.  Using ELISA and PCR to test the potential for spread of plum pox virus by seeds of different stone fruit cultivars , 2003 .

[304]  P. Martínez-Gómez,et al.  Localisation and movement of Plum pox virus in apricot stem tissues , 2003 .

[305]  H. Prieto,et al.  Biological Behavior and Partial Molecular Characterization of Six Chilean Isolates of Plum pox virus. , 2003, Plant disease.

[306]  J. Valkonen,et al.  Localization of a potyvirus and the viral genome-linked protein in wild potato leaves at an early stage of systemic infection. , 2003, Molecular plant-microbe interactions : MPMI.

[307]  J. Valkonen,et al.  Detection of the Potyviral Genome-Linked Protein VPg in Virions and Its Phosphorylation by Host Kinases , 2002, Journal of Virology.

[308]  M. Cambra,et al.  Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses. , 2002, Journal of virological methods.

[309]  M. Pfosser,et al.  Phylogeny and geographical differentiation of zucchini yellow mosaic virus isolates (Potyviridae) based on molecular analysis of the coat protein and part of the cytoplasmic inclusion protein genes , 2002, Archives of Virology.

[310]  S. Youssef,et al.  DETECTION AND IDENTIFICATION OF PRUNE DWARF VIRUS AND PLUM POX VIRUS BY STANDARD AND MULTIPLEX RT-PCR PROBE CAPTURE HYBRIDIZATION (RT-PCR-ELISA) , 2002 .

[311]  B. Moury,et al.  Molecular variability of the P3-6K1 genomic region among geographically and biologically distinct isolates of Plum pox virus , 2002, Archives of Virology.

[312]  N. Katis,et al.  Fluctuations in concentration of two potyviruses in garlic during the growing period and sampling conditions for reliable detection by ELISA , 2002 .

[313]  J. Valkonen,et al.  Viral genome-linked protein (VPg) controls accumulation and phloem-loading of a potyvirus in inoculated potato leaves. , 2002, Molecular plant-microbe interactions : MPMI.

[314]  C. Hjulsager,et al.  A new pathotype of Pea seedborne mosaic virus explained by properties of the P3-6k1- and viral genome-linked protein (VPg)-coding regions. , 2002, Molecular plant-microbe interactions : MPMI.

[315]  D. Burritt,et al.  Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. , 2002, Physiologia plantarum.

[316]  J. Valkonen,et al.  Coat protein gene-mediated resistance to Potato virus A in transgenic plants is suppressed following infection with another potyvirus. , 2001, The Journal of general virology.

[317]  I. E. Johansen,et al.  Mutations in pea seedborne mosaic virus genome-linked protein VPg after pathotype-specific virulence in Pisum sativum. , 2001, Molecular plant-microbe interactions : MPMI.

[318]  N. Minoiu,et al.  CURRENT SITUATION OF PLUM POX DISEASE ON STONE FRUIT SPECIES IN MIDDLE AND EASTERN EUROPE , 2001 .

[319]  E. Zintzaras,et al.  MONITORING AND SPATIOTEMPORAL ANALYSIS OF PPV-M SPREAD IN TWO APRICOT ORCHARDS IN SOUTHERN GREECE , 2001 .

[320]  F. Paprštein,et al.  LONG TERM EVALUATION OF PLUM CULTIVARS ARTIFICIALLY INFECTED BY PPV , 2001 .

[321]  L. Palkovics,et al.  MOLECULAR CHARACTERIZATION OF PLUM POX VIRUS ALMOND ISOLATE , 2001 .

[322]  J. Polák European spindle tree and common privet a new natural hosts of plum pox virus. , 2001 .

[323]  J. Valkonen,et al.  Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. , 2001, The Journal of general virology.

[324]  D. Boscia,et al.  Integrated RT-PCR/nested PCR diagnosis for differentiating between subgroups of plum pox virus. , 2001, Journal of virological methods.

[325]  P. Martínez-Gómez,et al.  Response of antioxidative enzymes to plum pox virus in two apricot cultivars. , 2001, Physiologia plantarum.

[326]  H. Sánchez-Sánchez,et al.  Identification of Wheat streak mosaic virus and Its Vector Aceria tosichella in Mexico. , 2001, Plant disease.

[327]  D. Boscia,et al.  First record of Plum pox virus in Jordan. , 2001 .

[328]  J. Laliberté,et al.  Complex Formation between Potyvirus VPg and Translation Eukaryotic Initiation Factor 4E Correlates with Virus Infectivity , 2000, Journal of Virology.

[329]  J. García,et al.  Preservation of 5'-end integrity of a potyvirus genomic RNA is not dependent on template specificity. , 2000, Virology.

[330]  J. Riechmann,et al.  Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K(1). , 2000, The Journal of general virology.

[331]  J. Valkonen,et al.  Incompatible potyvirus-host interactions in resistant plants: the viral genome-linked protein (VPg) of Potato virus A as an avirulence determinant. , 2000 .

[332]  T. Pirone,et al.  Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. , 1999, The Journal of general virology.

[333]  J. Valkonen,et al.  The 6K2 protein and the VPg of potato virus A are determinants of systemic infection in Nicandra physaloides. , 1999, Molecular plant-microbe interactions : MPMI.

[334]  D. Stenger,et al.  Fully biologically active in vitro transcripts of the eriophyid mite-transmitted wheat streak mosaic tritimovirus. , 1999, Phytopathology.

[335]  J. Carrington,et al.  Functional Analysis of the Interaction between VPg-Proteinase (NIa) and RNA Polymerase (NIb) of Tobacco Etch Potyvirus, Using Conditional and Suppressor Mutants , 1999, Journal of Virology.

[336]  Z. Šubr,et al.  Preparation of diagnostic monoclonal antibodies against two potyviruses. , 1999, Acta virologica.

[337]  A. Fereres,et al.  Aphid Attraction and Preference for Soybean and Pepper Plants Infected with Potyviridae , 1999 .

[338]  A. Aboul-Ela,et al.  PLUM POX POTYVIRUS SITUATION IN EGYPTIAN STONE FRUIT TREES , 1999 .

[339]  A. Maule,et al.  New Advances in Understanding the Molecular Biology of Plant/Potyvirus Interactions , 1999 .

[340]  J. Valkonen,et al.  Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus-host interactions and the same CP motif affects virus transmission and accumulation. , 1999, The Journal of general virology.

[341]  Adams,et al.  Plum pox virus detection in dormant plum trees by PCR and ELISA , 1999 .

[342]  D. Peters,et al.  The Differential Reactivity of Two Polyclonal Antisera to Plum Pox Virus Isolates Collected at Different Stages After Immunization , 1999 .

[343]  A. Maule,et al.  Specificity of resistance to pea seed-borne mosaic potyvirus in transgenic peas expressing the viral replicase (Nlb) gene. , 1998, The Journal of general virology.

[344]  V. Hari,et al.  STUDIES ON AN APRICOT STRAIN OF PLUM POX POTYVIRUS ISOLATED FROM EL AMAR, EGYPT , 1998 .

[345]  M. Navrátil,et al.  DETECTION AND SEROLOGICAL IDENTIFICATION OF PLUM POX VIRUS ISOLATES IN THE CZECH REPUBLIC , 1998 .

[346]  J. Hammond,et al.  A BROAD-SPECTRUM PCR ASSAY COMBINED WITH RFLP ANALYSIS FOR DETECTION AND DIFFERENTIATION OF PLUM POX VIRUS ISOLATES , 1998 .

[347]  R. Scorza,et al.  Challenging transgenic plums expressing potyvirus coat protein genes with viruliferous aphids. , 1998 .

[348]  R. Scorza,et al.  Transferring potyvirus coat protein genes through hybridization of transgenic plants to produce plum pox virus resistant plums (Prunus domestica L.). , 1998 .

[349]  P. Hinrichsen,et al.  Molecular characterization of plum pox virus isolated from apricots, plums and peaches in Chile. , 1998 .

[350]  A. Hadidi,et al.  Molecular evidence for the occurrence of plum pox virus - cherry subgroup in Hungary. , 1998 .

[351]  W. E. Howell,et al.  The versatility of Prunus tomentosa as a bioindicator of viruses. , 1998 .

[352]  E. Rozpara,et al.  THE RESPONSE OF SOME NEW PLUM CULTIVARS TO PLUM POX VIRUS (PPV) , 1998 .

[353]  M. Navrátil,et al.  Results of plum pox virus transmission into germplasm of plums. , 1998 .

[354]  K. Lehto,et al.  Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells. , 1998, The Journal of general virology.

[355]  A. Hunt,et al.  The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection. , 1998 .

[356]  P. Komínek,et al.  Restriction fragment length polymorphism differentiation of plum pox virus isolates. , 1998, Acta virologica.

[357]  M. Glasa,et al.  Characterization of plum pox virus isolates from Slovakia. , 1998, Acta virologica.

[358]  M. Navrátil,et al.  Molecular variability of Czech plum pox virus isolates. , 1998, Acta virologica.

[359]  T. Candresse,et al.  Production of a monoclonal antibody specific to the El Amar strain of plum pox virus. , 1998, Acta virologica.

[360]  A. Maule,et al.  Ultrastructural and temporal observations of the potyvirus cylindrical inclusions (Cls) show that the Cl protein acts transiently in aiding virus movement. , 1998, Virology.

[361]  J. Carrington,et al.  Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movement. , 1998, The Plant journal : for cell and molecular biology.

[362]  C. Martin,et al.  Peer reviewed: nanomaterials in analytical chemistry. , 1998, Analytical chemistry.

[363]  I. E. Johansen,et al.  A single conserved amino acid in the coat protein gene of pea seed-borne mosaic potyvirus modulates the ability of the virus to move systemically in Chenopodium quinoa. , 1998, Virology.

[364]  J. Dale,et al.  Sequence diversity in the coat protein coding region of twelve sugarcane mosaic potyvirus isolates from Australia, USA and South Africa , 1998, Archives of Virology.

[365]  K. Ryu,et al.  Nucleotide sequence analysis of the coat protein genes of two Korean isolates of sweet potato feathery mottle potyvirus , 1998, Archives of Virology.

[366]  B. Cassidy,et al.  Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency , 1998, Archives of Virology.

[367]  E. Maiss,et al.  Ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus , 1998, Archives of Virology.

[368]  T. Pirone,et al.  Charge changes near the N terminus of the coat protein of two potyviruses affect virus movement. , 1998, The Journal of general virology.

[369]  A. Bavoso,et al.  Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein. , 1997, Journal of virological methods.

[370]  M. Glasa,et al.  Susceptibility of peach GF 305 seedlings and selected herbaceous plants to plum pox virus isolates from western Slovakia. , 1997, Acta virologica.

[371]  T. Candresse,et al.  Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA. , 1997, Journal of virological methods.

[372]  J. García,et al.  Cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA. , 1997, The Journal of general virology.

[373]  Paul Linstead,et al.  The coat and cylindrical inclusion proteins of a potyvirus are associated with connections between plant cells. , 1997, Virology.

[374]  L. Giunchedi,et al.  Specific detection of D- and M-isolates of plum pox virus by immunoenzymatic determination of PCR products. , 1997, Journal of virological methods.

[375]  Z. Kiraly,et al.  Local and Systemic Responses of Antioxidants to Tobacco Mosaic Virus Infection and to Salicylic Acid in Tobacco (Role in Systemic Acquired Resistance) , 1997, Plant physiology.

[376]  D. Boscia,et al.  Characterization of the Sweet Cherry Isolate of Plum Pox Potyvirus. , 1997, Plant disease.

[377]  J. García,et al.  Long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. , 1997, Virology.

[378]  D. Colinet,et al.  Evidence for the assignment of two strains of SPLV to the genus Potyvirus based on coat protein and 3' non-coding region sequence data. , 1997, Virus research.

[379]  T. Pirone,et al.  A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. , 1997, Virology.

[380]  W. E. Howell,et al.  Prunus tomentosa as a Diagnostic Host for Detection of Plum Pox Virus and Other Prunus Viruses. , 1997, Plant disease.

[381]  L. Zhang,et al.  Immunocytology shows the presence of tobacco etch virus P3 protein in nuclear inclusions. , 1997, Journal of structural biology.

[382]  A. Hoffmann,et al.  Localization of plum pox virus (PPV, sharka) in plum (Prunus domestica L.) using tissue print-immunoblotting (TPIB) , 1997 .

[383]  P. Valdez,et al.  Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa) , 1997, Journal of virology.

[384]  W. Hartmann,et al.  Lokalisierung des Scharka-Virus bei Zwetschen (Prunus domestica L.) mit Hilfe des tissue print-immunoblotting (TPIB) , 1997 .

[385]  R. Gardner,et al.  Resistance to Vanilla Necrosis Potyvirus in Transgenic Nicotiana benthamiana Plants Containing the Virus Coat Protein Gene , 1997 .

[386]  J. Polák The Role of Prunus Spinosa L. in Epidemiology of Plum Pox Virus in the Czech Republic , 1997 .

[387]  D. Ducasse,et al.  Sequencing and characterization of the coat protein and 3′ non-coding region of a newsweet potato potyvirus , 1997, Archives of Virology.

[388]  Vincent C. Emery,et al.  J. Virol. Methods , 1996 .

[389]  J. Carrington,et al.  Nla and Nlb of peanut stripe potyvirus are present in the nucleus of infected cells, but do not form inclusions. , 1996, Virology.

[390]  V. Savino,et al.  SANITARY STATUS AND SANITATION OF STONE FRUIT TREES IN SOUTH EAST ITALY , 1996 .

[391]  E. Carbonell,et al.  Genetic diversity in apricot, Prunus armeniaca, aimed at improving resistance to plum pox virus , 1996 .

[392]  H. Baumgartnerová First findings of plum pox virus in walnut trees (Juglans Regia L.) , 1996, Acta virologica.

[393]  A. Hadidi,et al.  Characterization of the sour cherry strain of plum pox virus , 1996 .

[394]  T. Candresse,et al.  Sour Cherry Strain of Plum Pox Potyvirus (PPV): Molecular and Serological Evidence for a New Subgroup of PPV Strains , 1996 .

[395]  J. Shaw,et al.  A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells. , 1995, Virology.

[396]  T. Canto,et al.  Transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus Y helper component. , 1995, The Journal of general virology.

[397]  E. Carbonell,et al.  CHARACTERIZATION AND DETECTION OF PLUM POX VIRUS USING MONOCLONAL ANTIBODIES , 1995 .

[398]  V. Bălan,et al.  SUSCEPTIBILITY OF APRICOT, NECTARINE AND PEACH CULTIVARS AND HYBRIDS TO PLUM-POX VIRUS , 1995 .

[399]  A. Hadidi,et al.  3'non-coding region RT-PCR detection and molecular hybridization of plum pox virus in anthers of infected stone fruit , 1995 .

[400]  J. Riechmann,et al.  Processing of the plum pox virus polyprotein at the P3-6K1 junction is not required for virus viability. , 1995, The Journal of general virology.

[401]  M. Nuzzaci,et al.  Plum pox virus (PPV) in sweet cherry. , 1995 .

[402]  F. Dicenta,et al.  Localisation of plum pox virus in apricot stem and petiole tissues by tissue printing onto nitrocellulose membrane , 1995 .

[403]  C. Atreya,et al.  Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids. , 1995, The Journal of general virology.

[404]  J. Valkonen,et al.  Three Strain Groups of Potato A Potyvirus Based on Hypersensitive Responses in Potato, Serological Properties, and Coat Protein Sequences , 1995 .

[405]  E. Maiss,et al.  Molecular analyses of the coat protein region of different viruses on Poaceae belonging to the Potyviridae , 1995 .

[406]  R. S. Nelson,et al.  Differences in Protection Phenotypes in Tobacco Plants Expressing Coat Protein Genes from Peanut Stripe Potyvirus With or Without an Engineered ATG , 1995 .

[407]  C. Vela,et al.  Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins , 1994 .

[408]  T. Candresse,et al.  Detection of plum pox potyvirus and analysis of its molecular variability using immunocapture-PCR. , 1994 .

[409]  F. Rabenstein,et al.  Serological Detection of Members of the Potyviridae with Polyclonal Antisera , 1994 .

[410]  D. Matthews,et al.  Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein , 1994, Cell.

[411]  M. Restrepo-Hartwig,et al.  The tobacco etch potyvirus 6-kilodalton protein is membrane associated and involved in viral replication , 1994, Journal of virology.

[412]  L. Paulin,et al.  The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses. , 1994, The Journal of general virology.

[413]  M. Restrepo-Hartwig,et al.  Internal cleavage and trans-proteolytic activities of the VPg-proteinase (NIa) of tobacco etch potyvirus in vivo , 1993, Journal of virology.

[414]  M. Navrátil,et al.  Monoclonal antibodies suitable for plum pox virus determination. , 1993, Hybridoma.

[415]  G. Massonie,et al.  Experimental transmission of plum pox virus by Brachycaudus persicae Passerini [peach seedling] , 1993 .

[416]  A. Fos,et al.  Transmission expérimentale du virus de la sharka par Brachycaudus persicae Passerini , 1993 .

[417]  M. Cervera,et al.  Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling , 1992 .

[418]  M. Restrepo-Hartwig,et al.  Regulation of nuclear transport of a plant potyvirus protein by autoproteolysis , 1992, Journal of virology.

[419]  T. Candresse,et al.  A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. , 1992, Journal of virological methods.

[420]  E. Hiebert,et al.  Serological relationships involving potyviral nonstructural proteins. , 1992, Archives of virology. Supplementum.

[421]  J. Riechmann,et al.  Highlights and prospects of potyvirus molecular biology. , 1992, The Journal of general virology.

[422]  E. Koonin,et al.  The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase. , 1991, Virology.

[423]  J. Riechmann,et al.  Identification of the initiation codon of plum pox potyvirus genomic RNA. , 1991, Virology.

[424]  J. Fernández-Piqueras,et al.  Peroxidase isozyme analysis of factors involved in development of symptoms in Nicotiana clevelandii infected by plum pox virus , 1991 .

[425]  J. Fernández-Piqueras,et al.  Comparison among the isozyme profiles associated with ethrel treatments of leaves, and with senescence and plum pox virus infection in Chenopodium foetidum , 1991 .

[426]  T. Candresse,et al.  A polymerase chain reaction assay adapted to plum pox potyvirus detection. , 1991, Journal of virological methods.

[427]  T. Candresse,et al.  Nucleotide sequence of the 3'-terminal region of the RNA of the El Amar strain of plum pox potyvirus. , 1991, The Journal of general virology.

[428]  H. Katinger,et al.  A PCR membrane spot assay for the detection of plum pox virus RNA in bark of infected trees. , 1991, Journal of virological methods.

[429]  C. Ward,et al.  Taxonomy of potyviruses: current problems and some solutions. , 1991, Intervirology.

[430]  T. Candresse,et al.  Dot hybridization detection of plum pox virus using 32P-labeled RNA probes representing non-structural viral protein genes. , 1990, Journal of virological methods.

[431]  J. Carrington,et al.  Nuclear transport of plant potyviral proteins. , 1990, The Plant cell.

[432]  J. Carrington,et al.  Cap-independent enhancement of translation by a plant potyvirus 5' nontranslated region , 1990, Journal of virology.

[433]  I. Kamenova,et al.  Plum pox virus ppv , 1990 .

[434]  J. Fernández-Piqueras,et al.  Isozyme profiles associated with the hypersensitive response of Chenopodium foetidum to plum pox virus infection , 1990 .

[435]  R. Delbos,et al.  The complete nucleotide sequence of plum pox virus RNA (strain D). , 1989, Nucleic acids research.

[436]  H. Katinger,et al.  Early screening for anti-plum pox virus monoclonal antibodies with different epitope specificities by means of gold-labelled immunosorbent electron microscopy. , 1988, Journal of virological methods.

[437]  C. Sauter,et al.  Immunofluorescence localization of plum rox virus and prunus necrotic ringspot virus in tissue sections of Prunus cerasus and Prunus juxa , 1988 .

[438]  T. Candresse,et al.  Use of a 32P-labeled transcribed RNA probe for dot hybridization detection of plum pox virus , 1988 .

[439]  E. Fuchs,et al.  Non-transmission of the plum pox virus in plum and apricot seed source clones , 1988 .

[440]  V. Schuster,et al.  Use of peroxidase labelled antibodies for detection of plum pox virus. , 1981 .

[441]  J. Kröll [Verification of the plum pox virus (PPV) using the tray test (author's transl)]. , 1978, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abteilung: Mikrobiologie der Landwirtschaft der Technologie und des Umweltschutzes.

[442]  J. Kröll [Experiments to verify the plum pox virus (PPV) by communicating it on Chenopodium foetidum Schrad. (author's transl)]. , 1975, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abt.: Allgemeine, landwirtschaftliche und technische Mikrobiologie.

[443]  E. Hiebert,et al.  Partial purification and some properties of tobacco etch virus induced intranuclear inclusions. , 1974, Virology.

[444]  J. Shepard Electron microscopy of subtilisin-treated tobacco etch virus nuclear and cytoplasmic inclusions. , 1968, Virology.

[445]  Z. Kiraly,et al.  Role of oxidative metabolism in the localization of plant viruses. , 1960, Virology.

[446]  F. Sheffield II.—THE CYTOPLASMIC AND NUCLEAR INCLUSIONS ASSOCIATED WITH SEVERE ETCH VIRUS , 1941 .

[447]  D. Atanassov Plum pox. A new virus disease. , 1932 .

[448]  I. O. Elibuyuk Detection of Plum Pox Virus in Ornamental Prunus cerasifera , 2022 .