Automatic processing and interpretation of DNA distributions: comparison of several techniques.

Abstract There exist a large number of apparently different methods for interpreting the distribution of DNA content in a population of cells as measured by an impulse cytophotometer (FMF). Equations are derived to provide a model of the observed numbers of cells with a given DNA content. Methods are described to compute the “ideal” distribution of cells necessary to find the number of cells in G1, S and G2 + M phase. A comparison is made of several standard methods for computing the cell age distribution. The methods are shown to be formally nearly equivalent and to give similar results when applied to an in vitro control study.

[1]  D. Prescott,et al.  Buoyant density of DNA synthesized at different stages of the S phase of mouse L cells. , 1971, Experimental cell research.

[2]  H. Edenberg,et al.  Eukaryotic chromosome replication. , 1975, Annual review of genetics.

[3]  I. Tamm,et al.  DNA REPLICATION: DIRECTION AND RATE OF CHAIN GROWTH IN MAMMALIAN CELLS , 1973, The Journal of cell biology.

[4]  W. Göhde,et al.  Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle , 1975, Radiation and environmental biophysics.

[5]  S. I. Rubinow,et al.  A maturity-time representation for cell populations. , 1968, Biophysical journal.

[6]  J. H. Taylor,et al.  Replication of DNA in mammalian chromosomes. IV. Partial characterization of DNA fragments released from replicating DNA of CHO cells. , 1977, Experimental cell research.

[7]  J. Fried.  Method for the quantitative evaluation of data from flow microfluorometry. , 1976, Computers and biomedical research, an international journal.

[8]  E J Freireich,et al.  The effect of adriamycin on the cell cycle traverse of a human lymphoid cell line. , 1976, Cancer research.

[9]  B. Drewinko,et al.  Harvesting methods of immunoglobulin producing cells. , 1971, Revue europeenne d'etudes cliniques et biologiques. European journal of clinical and biological research.

[10]  David G. Kendall,et al.  ON THE ROLE OF VARIABLE GENERATION TIME IN THE DEVELOPMENT OF A STOCHASTIC BIRTH PROCESS , 1948 .

[11]  G. Steel,et al.  CELL LOSS FROM EXPERIMENTAL TUMOURS , 1968 .

[12]  B. Drewinko,et al.  The ability of tumor cells of the lymphoreticular system to grow in vitro. , 1972, Cancer research.

[13]  J. Gray.  CELL‐CYCLE ANALYSIS OF PERTURBED CELL POPULATIONS: COMPUTER SIMULATION OF SEQUENTIAL DNA DISTRIBUTIONS , 1976, Cell and tissue kinetics.

[14]  J. R. Roti Roti,et al.  A MATHEMATICAL MODEL OF THE CELL CYCLE OF L5178Y , 1973, Cell and tissue kinetics.

[15]  H. Ozer,et al.  Replication kinetics of three DNA sequence families in synchronized mouse cells , 1977, Journal of cellular physiology.

[16]  B. Barlogie,et al.  Pulse cytophotometric analysis of synchronized cells in vitro. , 1976, Cancer research.

[17]  J. Jett,et al.  MATHEMATICAL ANALYSIS OF DNA DISTRIBUTIONS DERIVED FROM FLOW MICROFLUOROMETRY , 1974, The Journal of cell biology.

[18]  R. Hand.  Regulation of DNA replication on subchromosomal units of mammalian cells , 1975, The Journal of cell biology.

[19]  Carl F. Evert.  CELLDYN - a digital program for modeling the dynamics of cells , 1975 .

[20]  P. Walker.  The Mitotic Index and Interphase Processes , 1954 .

[21]  Manabu Takahashi,et al.  Theoretical basis for cell cycle analysis I. Labelled mitosis wave method , 1966 .

[22]  C E Donaghey,et al.  A computer simulation program for the study of cellular growth kinetics and its application to the analysis of human lymphoma cells in vitro. , 1975, Computers and biomedical research, an international journal.

[23]  G. Hahn.  State vector description of the proliferation of mammalian cells in tissue culture. I. Exponential growth. , 1966, Biophysical journal.

[24]  H. Tapiero,et al.  Replication of repetitious DNA in synchronized chick fibroblast cells. , 1974, Experimental cell research.

[25]  J. H. Taylor,et al.  Sequence programming of DNA replication over the S phase of Chinese hamster cells. , 1977, Experimental cell research.

[26]  K. Pearson.  Contributions to the Mathematical Theory of Evolution , 1894 .

[27]  H. Crissman,et al.  Cell-Cycle Analysis in 20 Minutes , 1974, Science.

[28]  P. Volpe,et al.  A method for measuring cell cycle phases in suspension cultures. , 1973, Methods in cell biology.

[29]  R. White,et al.  State-vector models of the cell cycle. 1. Parametrization and fits to labeled mitoses data. , 1977, Journal of theoretical biology.

[30]  R. Meyn,et al.  Evaluation of S phase synchronization by analysis of DNA replication in 5-bromodeoxyuridine. , 1973, Experimental cell research.