A facile method to clickable sensing polymeric nanoparticles.

Clickable biocompatible nanoparticles were prepared in a one-pot process by microemulsion polymerization using acrylamide, N,N'-methylene bisacrylamide and either N-(11-azido-3,6,9-trioxaundecanyl)acrylamide or N-propargylacrylamide, which were then readily modified by CuAAC reaction to afford sensing nanomaterials.

[1]  E. Vauthey,et al.  Protease responsive nanoprobes with tethered fluorogenic peptidyl 3-arylcoumarin substrates. , 2009, Chemical communications.

[2]  W. Binder,et al.  'Click' Chemistry in Polymer and Material Science: An Update , 2008 .

[3]  P. Lovell,et al.  Click chemistry as a route to surface functionalization of polymer particles dispersed in aqueous media. , 2009, Chemical communications.

[4]  Jonathan W Aylott,et al.  Optical calcium sensors: development of a generic method for their introduction to the cell using conjugated cell penetrating peptides. , 2005, The Analyst.

[5]  Morten Meldal,et al.  Cu-catalyzed azide-alkyne cycloaddition. , 2008, Chemical reviews.

[6]  C. Hawker,et al.  Orthogonal approaches to the simultaneous and cascade functionalization of macromolecules using click chemistry. , 2005, Journal of the American Chemical Society.

[7]  Morten Meldal,et al.  Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides. , 2002, The Journal of organic chemistry.

[8]  Hong Gu,et al.  Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes , 2006 .

[9]  Dong Wang,et al.  Click Chemistry, A Powerful Tool for Pharmaceutical Sciences , 2008, Pharmaceutical Research.

[10]  Barrie W. Bycroft,et al.  An appraisal of new variants of Dde amine protecting group for solid phase peptide synthesis , 1998 .

[11]  K. Sharpless,et al.  Polytriazoles as copper(I)-stabilizing ligands in catalysis. , 2004, Organic letters.

[12]  H. Nakayama,et al.  New fluorogenic substrates for subtilisin. , 1985, Chemical & pharmaceutical bulletin.

[13]  M. G. Finn,et al.  Click Chemistry: Diverse Chemical Function from a Few Good Reactions. , 2001, Angewandte Chemie.

[14]  P. Coupland,et al.  Using fluorescent pH-sensitive nanosensors to report their intracellular location after Tat-mediated delivery. , 2009, Integrative biology : quantitative biosciences from nano to macro.