SIMULTANEOUS DETERMINATION OF TOLPERISONE AND PARACETAMOL IN PURE AND FIXED DOSE COMBINATION BY UV - SPECTROPHOTOMETRY

Two new simple accurate and sensitive UV– spectrophotometeric methods have been developed for determination Simultaneous equation and Derivative spectroscopy method for Tolperisone and Paracetamol in bulk and in combined dosage form. Double distilled was used as a solvent. The wavelength selected for Simultaneous method for Tolperisone at 261nm and Paracetamol at 243nm respectively. Beer’s law was obeyed with the concentration ranges from 0-2.5µg/ml, 3- 9µg/ml respectively. For derivative spectroscopy method(1-5µg/ml,6-30µg/ml) the zero order spectrum was derivatized to first order derivative with the zero crossing points of Tolperisone has maxima at 243nm and Paracetamol has maxima at 261nm.The % recovery was found in the range 99.0 ± 0.012, 100 ± 0.342.The developed method was validated statically by recovery studies. The %R.S.D was found to be less than 2. Thus the proposed method was simple, precise, economic, rapid, accurate and successfully applied for simultaneous determination.