Electrochemiluminescence detection of methamphetamine in biological matrices

We have studied an electrochemical detection method for the stimulants in the forensic samples using electrochemiluminescence (ECL). In this context, amphetamine type stimulant (methamphetamine (MA)) has been studied as coreactants in the [Ru(bpy)3]2+ (where bpy is 2,2’-bipyridine) ECL system. This approach is developed based on a glassy carbon electrode modified with [Ru(bpy)3]2+ /Nafion composite film. LoD, LoQ and linear working range for MA are studied currently. The ECL intensity was found to be concentrations over the range of 5 x 10-8 to 2.5 x 10-4mol/L. LoD for MA is 1.94 x 10-10 mol/L. The regression coefficient is 0.9931 for the experiment. Our approach was applied in different medium such as saliva and human serum to detect MA This technique is simple, rapid, selective and sensitive, and shows potential for the highthroughput quantitation of MA. the results show that the present electrochemical approach seems to provide a sensitive detection of MA in forensic applications.

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