Monte Carlo model of the penetration depth for polarization gating spectroscopy: influence of illumination-collection geometry and sample optical properties.

Polarization-gating has been widely used to probe superficial tissue structures, but the penetration depth properties of this method have not been completely elucidated. This study employs a polarization-sensitive Monte Carlo method to characterize the penetration depth statistics of polarization-gating. The analysis demonstrates that the penetration depth depends on both the illumination-collection geometry [illumination-collection area (R) and collection angle (θ(c))] and on the optical properties of the sample, which include the scattering coefficient (μ(s)), absorption coefficient (μ(a)), anisotropy factor (g), and the type of the phase function. We develop a mathematical expression relating the average penetration depth to the illumination-collection beam properties and optical properties of the medium. Finally, we quantify the sensitivity of the average penetration depth to changes in optical properties for different geometries of illumination and collection. The penetration depth model derived in this study can be applied to optimizing application-specific fiber-optic probes to target a sampling depth of interest with minimal sensitivity to the optical properties of the sample.

[1]  Vadim Backman,et al.  Assessment of blood supply in superficial tissue by polarization-gated elastic light-scattering spectroscopy. , 2006, Applied optics.

[2]  Jessica Ramella-Roman,et al.  Three Monte Carlo programs of polarized light transport into scattering media: part I. , 2005, Optics express.

[3]  Joshua Pfefer,et al.  Depth-sensitive reflectance measurements using obliquely oriented fiber probes. , 2005, Journal of biomedical optics.

[4]  Melissa C Skala,et al.  Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers , 2004, Lasers in surgery and medicine.

[5]  Vadim Backman,et al.  Rectal Mucosal Microvascular Blood Supply Increase Is Associated with Colonic Neoplasia , 2009, Clinical Cancer Research.

[6]  Mia K Markey,et al.  Probing local tissue changes in the oral cavity for early detection of cancer using oblique polarized reflectance spectroscopy: a pilot clinical trial. , 2008, Journal of biomedical optics.

[7]  Vadim Backman,et al.  Simultaneous measurement of angular and spectral properties of light scattering for characterization of tissue microarchitecture and its alteration in early precancer , 2003 .

[8]  Michael S. Feld,et al.  Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ , 1999 .

[9]  Da Xing,et al.  Determination of optical properties of normal and adenomatous human colon tissues in vitro using integrating sphere techniques. , 2005, World journal of gastroenterology.

[10]  Linda T. Nieman,et al.  Fiber optic probe for polarized reflectance spectroscopy in vivo: design and performance. , 2002, Journal of biomedical optics.

[11]  V. Backman,et al.  Increased microvascular blood content is an early event in colon carcinogenesis , 2005, Gut.

[12]  Yang Liu,et al.  Investigation of depth selectivity of polarization gating for tissue characterization. , 2005, Optics express.

[13]  Arjen Amelink,et al.  Measurement of the local optical properties of turbid media by differential path-length spectroscopy. , 2004, Applied optics.

[14]  M. H. Koelink,et al.  Condensed Monte Carlo simulations for the description of light transport. , 1993, Applied optics.

[15]  R. Anderson.  Polarized light examination and photography of the skin. , 1991, Archives of dermatology.

[16]  A. Welch,et al.  Determining the optical properties of turbid mediaby using the adding-doubling method. , 1993, Applied optics.

[17]  Hervé Saint-Jalmes,et al.  Description and time reduction of a Monte Carlo code to simulate propagation of polarized light through scattering media. , 2003, Applied optics.

[18]  Rebecca Richards-Kortum,et al.  Reflectance spectroscopy for diagnosis of epithelial precancer: model-based analysis of fiber-optic probe designs to resolve spectral information from epithelium and stroma. , 2005, Applied optics.

[19]  M. H. Koelink,et al.  Reduced light-scattering properties for mixtures of spherical particles: a simple approximation derived from Mie calculations. , 1992, Applied optics.

[20]  Allen Taflove,et al.  A predictive model of backscattering at subdiffusion length scales , 2010, Biomedical optics express.

[21]  J. Pawley,et al.  Handbook of Biological Confocal Microscopy , 1990, Springer US.

[22]  Vadim Backman,et al.  Development of a bioengineered tissue model and its application in the investigation of the depth selectivity of polarization gating. , 2005, Applied optics.

[23]  Jessica C Ramella-Roman,et al.  Imaging skin pathology with polarized light. , 2002, Journal of biomedical optics.

[24]  Arjen Amelink,et al.  In vivo measurement of the local optical properties of tissue by use of differential path-length spectroscopy. , 2004, Optics letters.

[25]  Linda T. Nieman,et al.  Optical sectioning using a fiber probe with an angled illumination-collection geometry: evaluation in engineered tissue phantoms. , 2004, Applied optics.

[26]  S. Jacques,et al.  Imaging Superficial Tissues With Polarized Light , 2000 .

[27]  Anant Agrawal,et al.  Oblique-incidence illumination and collection for depth-selective fluorescence spectroscopy. , 2005, Journal of biomedical optics.

[28]  S L Jacques,et al.  Measurement of tissue optical properties by the use of oblique-incidence optical fiber reflectometry. , 1997, Applied optics.

[29]  Nirmala Ramanujam,et al.  Relationship between depth of a target in a turbid medium and fluorescence measured by a variable-aperture method. , 2002, Optics letters.

[30]  T Joshua Pfefer,et al.  Multiple-fiber probe design for fluorescence spectroscopy in tissue. , 2002, Applied optics.

[31]  Vadim Backman,et al.  Neo-angiogenesis and the premalignant micro-circulatory augmentation of early colon carcinogenesis. , 2011, Cancer letters.

[32]  T Joshua Pfefer,et al.  Selective detection of fluorophore layers in turbid media: the role of fiber-optic probe design. , 2003, Optics letters.

[33]  Ji Yi,et al.  Polarized Enhanced Backscattering Spectroscopy for Characterization of Biological Tissues at Subdiffusion Length Scales , 2012, IEEE Journal of Selected Topics in Quantum Electronics.

[34]  Alex Vitkin,et al.  Monte Carlo study of pathlength distribution of polarized light in turbid media. , 2007, Optics express.

[35]  Rebecca Richards-Kortum,et al.  Ball lens coupled fiber-optic probe for depth-resolved spectroscopy of epithelial tissue. , 2005, Optics letters.

[36]  Linda T. Nieman,et al.  Compact beveled fiber optic probe design for enhanced depth discrimination in epithelial tissues. , 2009, Optics express.

[37]  R. Alfano,et al.  Optical polarization imaging. , 1997, Applied optics.

[38]  Nirmala Ramanujam,et al.  Effect of fiber optic probe geometry on depth-resolved fluorescence measurements from epithelial tissues: a Monte Carlo simulation. , 2003, Journal of biomedical optics.

[39]  Roberto Reif,et al.  Analytical model of light reflectance for extraction of the optical properties in small volumes of turbid media. , 2007, Applied optics.

[40]  Rebecca Richards-Kortum,et al.  Fluorescence spectroscopy of oral tissue: Monte Carlo modeling with site-specific tissue properties. , 2009, Journal of biomedical optics.

[41]  Vadim Backman,et al.  Nonscalar elastic light scattering from continuous random media in the Born approximation. , 2009, Optics letters.

[42]  Alex Vitkin,et al.  A Monte Carlo study of penetration depth and sampling volume of polarized light in turbid media , 2008 .

[43]  Masoud Panjehpour,et al.  Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements. , 2011, Gastroenterology.

[44]  Vadim Backman,et al.  Measuring mucosal blood supply in vivo with a polarization-gating probe. , 2008, Applied optics.

[45]  Scott A Prahl,et al.  Three Monte Carlo programs of polarized light transport into scattering media: part II. , 2005, Optics express.

[46]  Toshimitsu Asakura,et al.  Photon pathlength distribution from polarized backscattering in random media , 1996 .

[47]  Vadim Backman,et al.  Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk. , 2008, Gastroenterology.