Flexible dual-beam geometry for advanced optical micromanipulation experiments

We present a novel method for optical manipulation of microobjects and nanoobjects employing adaptive optical element to control properties of two counter-propagating beams overlapping in a sample chamber. We show that using this system one can eliminate optical aberrations in both pathways, online realign the system remotely from a computer interface, arbitrarily switch in real time between various beams types (i.e. Gaussian, Bessel, vortex) and their spatial intensity distributions (beam width, vorticity). We demonstrate optical manipulation of both high- and low-index particles in water or air, particle delivery in an optical conveyor belt using stationary mechanical components, formation of colloidal solitons, visualization of fluid flow in microcapillary as well as the rotation and reorientation of a trapped cell.

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