Production of Lactic Acid from Cellobiose and Cellotriose by Lactobacillus delbrueckii Mutant Uc-3

ABSTRACT Lactobacillus delbrueckii mutant Uc-3 utilizes both cellobiose and cellotriose efficiently, converting it into L(+) lactic acid. The enzyme activities of cellobiose and cellotriose utilization were determined for cell extracts, whole cells, and disrupted cells. Aryl-β-glucosidase activity was detected only for whole cells and disrupted cells, suggesting that these activities are cell bound. The mutant produced 90 g/liter of lactic acid from 100 g/liter of cellobiose with 2.25 g/liter/h productivity.

[1]  D. Gokhale,et al.  Lactic acid production from waste sugarcane bagasse derived cellulose , 2007 .

[2]  M. Fick,et al.  Lactic acid production by Bacillus coagulans—kinetic studies and optimization of culture medium for batch and continuous fermentations , 1999 .

[3]  F. Carr,et al.  The Lactic Acid Bacteria: A Literature Survey , 2002, Critical reviews in microbiology.

[4]  Mark Holtzapple,et al.  Coordinated development of leading biomass pretreatment technologies. , 2005, Bioresource technology.

[5]  M. Kanellaki,et al.  Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material. , 2003, Journal of agricultural and food chemistry.

[6]  J. Parajó,et al.  Multi-step feeding systems for lactic acid production by simultaneous saccharification and fermentation of processed wood , 2000 .

[7]  D. Gokhale,et al.  Polysaccharides from bagasse: applications in cellulase and xylanase production , 2004 .

[8]  G. Caminal,et al.  Kinetic modeling of the enzymatic hydrolysis of pretreated cellulose , 1985, Biotechnology and bioengineering.

[9]  B. Mattiasson,et al.  Lactic acid fermentation in a recycle batch reactor using immobilized Lactobacillus casei. , 1997, Biotechnology and bioengineering.

[10]  L. Lynd,et al.  A functionally based model for hydrolysis of cellulose by fungal cellulase , 2006, Biotechnology and bioengineering.

[11]  K. Nakasaki,et al.  Effects of intermittent addition of cellulase for production of L-lactic acid from wastewater sludge by simultaneous saccharification and fermentation. , 2003, Biotechnology and bioengineering.

[12]  S Abe,et al.  Simultaneous saccharification and fermentation of cellulose to lactic acid , 1991, Biotechnology and bioengineering.

[13]  M. Himmel,et al.  Outlook for cellulase improvement: screening and selection strategies. , 2006, Biotechnology advances.

[14]  K. Shanmugam,et al.  Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid , 2006, Applied and Environmental Microbiology.

[15]  John N. Saddler,et al.  Enzyme recycling during fed-batch hydrolysis of cellulose derived from steam-explodedEucalyptus viminalis , 1994 .

[16]  L. Xia,et al.  Kinetics of Simultaneous Saccharification and Lactic Acid Fermentation Processes , 1997, Biotechnology progress.

[17]  D. Gokhale,et al.  Strain improvement of Penicillium janthinellum NCIM 1171 for increased cellulase production. , 2007, Bioresource technology.

[18]  D. Gokhale,et al.  Strain improvement of Lactobacillus delbrueckii NCIM 2365 for lactic acid production , 2006 .

[19]  Ana Belén Moldes,et al.  Strategies to improve the bioconversion of processed wood into lactic acid by simultaneous saccharification and fermentation , 2001 .

[20]  K. Venkatesh Simultaneous saccharification and fermentation of cellulose to lactic acid , 1997 .

[21]  J. Parajó,et al.  Production of lactic acid from lignocellulose in a single stage of hydrolysis and fermentation , 1997 .