Development of a multi-band photoacoustic tomography imaging system based on a capacitive micromachined ultrasonic transducer array.

Photoacoustic tomography (PAT) as a hybrid technology combines the high optical contrast and high acoustic resolution in a single imaging modality. However, most of the available PAT systems cannot comprehensively or accurately characterize biological systems at multiple length scales due to the use of narrow bandwidth commercial ultrasonic transducers. In this study, we fabricated a novel multi-band capacitive micromachined ultrasonic transducer (CMUT) array, and first developed a CMUT-based multi-band photoacoustic tomography (MBPAT) imaging system. The MBPAT imaging system was examined by the phantom experiment, and then was successfully applied to image the zebrafish in vivo. The imaging results indicated that CMUT-array-based MBPAT can provide a more comprehensive and accurate characterization of biological tissues, which exhibit the potential of MBPAT/CMUT in various areas of biomedical imaging.

[1]  Lihong V. Wang,et al.  High-numerical-aperture-based virtual point detectors for photoacoustic tomography. , 2008, Applied physics letters.

[2]  R. Kruger,et al.  Photoacoustic ultrasound (PAUS)--reconstruction tomography. , 1995, Medical physics.

[3]  Zhen Yuan,et al.  Multi-spectral photoacoustic elasticity tomography. , 2016, Biomedical optics express.

[4]  Jie Yuan,et al.  The functional pitch of an organ: quantification of tissue texture with photoacoustic spectrum analysis. , 2014, Radiology.

[5]  B.T. Khuri-Yakub,et al.  Calculation and measurement of electromechanical coupling coefficient of capacitive micromachined ultrasonic transducers , 2003, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[6]  Lei Xi,et al.  Variable-thickness multilayered polyvinylidene fluoride transducer with improved sensitivity and bandwidth for photoacoustic imaging , 2012 .

[7]  Alexander Sampaleanu,et al.  Top orthogonal to bottom electrode (TOBE) 2-D CMUT arrays for 3-D photoacoustic imaging , 2014, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control.

[8]  Lihong V. Wang,et al.  Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs , 2012, Science.

[9]  Roger J. Zemp,et al.  Multifrequency Interlaced CMUTs for Photoacoustic Imaging , 2017, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control.

[10]  Lihong V. Wang,et al.  Photoacoustic imaging in biomedicine , 2006 .

[11]  A. F. Sarioglu,et al.  Singulation for imaging ring arrays of capacitive micromachined ultrasonic transducers , 2014, Journal of micromechanics and microengineering : structures, devices, and systems.

[12]  Lei Xi,et al.  Whole-body multispectral photoacoustic imaging of adult zebrafish. , 2016, Biomedical optics express.

[13]  John T. Wei,et al.  Quantifying Gleason scores with photoacoustic spectral analysis: feasibility study with human tissues. , 2015, Biomedical optics express.

[14]  Pai-Chi Li,et al.  A photoacoustic imager with light illumination through an infrared-transparent silicon CMUT array , 2012, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[15]  Da Xing,et al.  Characterization of lipid-rich aortic plaques by intravascular photoacoustic tomography: ex vivo and in vivo validation in a rabbit atherosclerosis model with histologic correlation. , 2014, Journal of the American College of Cardiology.

[16]  Butrus T. Khuri-Yakub,et al.  Capacitive Micromachined Ultrasonic Transducers: Theory and Technology , 2003 .

[17]  Xueding Wang,et al.  Interstitial photoacoustic spectral analysis: instrumentation and validation. , 2017, Biomedical optics express.

[18]  B.T. Khuri-Yakub,et al.  Finite-element analysis of capacitive micromachined ultrasonic transducers , 2005, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[19]  O. Oralkan,et al.  Capacitive micromachined ultrasonic transducers: fabrication technology , 2005, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[20]  Zhen Yuan,et al.  Long-term in vivo monitoring of injury induced brain regeneration of the adult zebrafish by using spectral domain optical coherence tomography , 2016 .

[21]  Geng Ku,et al.  Multiple-bandwidth photoacoustic tomography. , 2004, Physics in medicine and biology.

[22]  Jian Zhang,et al.  In vivo three-dimensional characterization of the adult zebrafish brain using a 1325 nm spectral-domain optical coherence tomography system with the 27 frame/s video rate. , 2015, Biomedical optics express.