Crystallization and preliminary X-ray analysis of human thioredoxin peroxidase-B from red blood cells.

Two different crystal forms of human thioredoxin peroxidase-B have been grown by vapour diffusion using polyethylene glycol 400 as a precipitant. Monoclinic P21 crystals were grown from freshly purified protein, whilst orthorhombic P212121 crystals were grown from purified protein that had been stored in ammonium sulfate, but otherwise under the same conditions. The diffraction from both crystal forms was observed to extend to beyond 2.0 A resolution using synchrotron radiation. Complete native data sets to 1.8 and 3. 7 A have been collected from the monoclinic and orthorhombic crystals, respectively.

[1]  C. Ponting,et al.  Porcine natural-killer-enhancing factor-B: oligomerisation and identification as a calpain substrate in vitro. , 1998, Biochimica et biophysica acta.

[2]  L. Obeid,et al.  Thioredoxin Peroxidase Is a Novel Inhibitor of Apoptosis with a Mechanism Distinct from That of Bcl-2* , 1997, The Journal of Biological Chemistry.

[3]  R. V. van Etten,et al.  The PAG gene product, a stress-induced protein with antioxidant properties, is an Abl SH3-binding protein and a physiological inhibitor of c-Abl tyrosine kinase activity. , 1997, Genes & development.

[4]  S. Richter,et al.  Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica. , 1997, The Biochemical journal.

[5]  D. O’Rourke,et al.  Murine thioredoxin peroxidase delays neuronal apoptosis and is expressed in areas of the brain most susceptible to hypoxic and ischemic injury. , 1997, DNA and cell biology.

[6]  S. Rhee,et al.  Thioredoxin-dependent peroxide reductase from yeast. , 1994, The Journal of biological chemistry.

[7]  R. Moore,et al.  Calcium-activated potassium transport and high molecular weight forms of calpromotin. , 1992, The Journal of biological chemistry.

[8]  Y. Matsui,et al.  Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. , 1990, Gene.

[9]  B. Ames,et al.  An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties. , 1989, The Journal of biological chemistry.

[10]  Z. Otwinowski,et al.  [20] Processing of X-ray diffraction data collected in oscillation mode. , 1997, Methods in enzymology.

[11]  C. Hedrick,et al.  Endogenous natural killer enhancing factor-B increases cellular resistance to oxidative stresses. , 1997, Free radical biology & medicine.