Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation.

In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy, spectra in a previously unexplored wavelength region 0.8-2.3 micron were recorded from various pig heart tissue samples in vitro: normal myocardium (with and without endo/epicardium), aorta, fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA), revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin, collagen and cholesterol as well as with published data. The results obtained with the NIR spectroscopy technique in terms of its potential to classify different tissue types were compared with those from laser-induced fluorescence (LIF) using 337 nm excitation. LIF and NIR spectroscopy can in combination with PCA be used to discriminate between all previously mentioned tissue groups, apart from fatty versus fibrous tissue (LIF) and aorta versus fibrous tissue (NIR), respectively. The NIR analysis was improved by focusing the PCA to the wavelength segment 2.0-2.3 microns, resulting in successful spectral characterisation of all cardiovascular tissue groups.

[1]  Henry H. Mantsch,et al.  Molecular spectroscopy in biodiagnostics (from Hippocrates to Herschel and beyond) , 1995 .

[2]  C. Salles,et al.  Determination of serum cholesterol by near-infrared reflectance spectrometry. , 1987, Analytical chemistry.

[3]  R. Rava,et al.  Biochemical analysis and mapping of atherosclerotic human artery using FT-IR microspectroscopy. , 1993, Atherosclerosis.

[4]  J. W. Hall,et al.  Near-infrared spectrophotometry: a new dimension in clinical chemistry. , 1992, Clinical chemistry.

[5]  D. F. Morrison,et al.  Multivariate Statistical Methods , 1968 .

[6]  K. Schomacker,et al.  Intraoperative myocardial ischemia detection with laser-induced fluorescence. , 1994, The Journal of thoracic and cardiovascular surgery.

[7]  Tsuyoshi Miura,et al.  Studies on Spectra/Structure Correlations in Near-Infrared Spectra of Proteins and Polypeptides. Part I: A Marker Band for Hydrogen Bonds , 1994 .

[8]  J. Tulip,et al.  Laser‐induced fluorescence emission: I. The spectroscopic identification of fibrotic endocardium and myocardium , 1991, Lasers in surgery and medicine.

[9]  A F Gmitro,et al.  Measurement depth of laser-induced tissue fluorescence with application to laser angioplasty. , 1988, Applied optics.

[10]  I J Bigio,et al.  Spectroscopic diagnosis of bladder cancer with elastic light scattering , 1995, Lasers in surgery and medicine.

[11]  L. Deckelbaum.  Cardiovascular applications of laser technology , 1994, Lasers in surgery and medicine.

[12]  J. Curcio,et al.  Near infrared absorption spectrum of liquid water , 1951 .

[13]  S L Jacques,et al.  XeCl laser-induced fluorescence of atherosclerotic arteries. Spectral similarities between lipid-rich lesions and peroxidized lipoproteins. , 1993, Circulation research.

[14]  R. Lodder,et al.  Near-infrared spectrophotometric monitoring of stroke-related changes in the protein and lipid composition of whole gerbil brains. , 1993, Analytical Chemistry.

[15]  K. Norris,et al.  A new approach for the estimation of body composition: infrared interactance. , 1984, The American journal of clinical nutrition.

[16]  H. Mantsch,et al.  Modification of the extracellular matrix following myocardial infarction monitored by FTIR spectroscopy. , 1996, Biochimica et biophysica acta.

[17]  E. Lanza.  Determination of Moisture, Protein, Fat, and Calories in Raw Pork and Beef By Near Infrared Spectroscopy , 1983 .

[18]  T. Papazoglou,et al.  Malignancies and atherosclerotic plaque diagnosis--is laser induced fluorescence spectroscopy the ultimate solution? , 1995, Journal of photochemistry and photobiology. B, Biology.

[19]  O Jarlman,et al.  Medical transillumination imaging using short-pulse diode lasers. , 1993, Applied optics.

[20]  L A Cassis,et al.  Near-IR imaging of atheromas in living arterial tissue. , 1993, Analytical chemistry.

[21]  F. Jöbsis.  Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. , 1977, Science.

[22]  J. W. Hall,et al.  Near-infrared spectroscopic determination of serum total proteins, albumin, globulins, and urea. , 1993, Clinical biochemistry.