Penetration of penicillin into human phagocytes containing Neisseria gonorrhoeae: intracellular survival and growth at optimum concentrations of antibiotic.

Phagocytes obtained from fresh human buffy coat (predominantly polymorphonuclear phagocytes) or from human buffy coat which had been incubated on a glass surface for 1 to 3 days (predominantly mononuclear phagocytes) were allowed to ingest gonococci, and then incubated with penicillin. More intracellular gonococci were killed at high than at low penicillin concentrations, indicating that penicillin penetrated the phagocytes. This was supported by autoradiography experiments with radiolabelled penicillin. A pilated, small-colony-forming gonococcal strain survived and multiplied for at least 15 h in polymorphonuclear phagocytes which were incubated with penicillin at the optimum concentration for killing the extracellular bacteria but not the intracellular ones; whereas a non-pilated, large-colony-forming strain survived for only 10 h. The former strain survived for at least 6 h in similar experiments with mononuclear phagocytes. Intracellular survival and in growth may be an important facet of the pathogenicity of gonococci.

[1]  H. Smith,et al.  Resistance of Neisseria gonorrhoeae to ingestion and digestion by phagocytes of human buffy coat. , 1976, Journal of medical microbiology.

[2]  J. Short,et al.  An electron-microscope study of naturally occurring and cultured cells of Neisseria Gonorrhoeae. , 1975, Journal of medical microbiology.

[3]  J. Brostoff,et al.  Intracellular killing of Listeria monocytogenes by activated macrophages (Mackaness system) is due to antibiotic , 1975, Nature.

[4]  K. Witt,et al.  DIFFERENTIAL ABILITY OF COLONIAL TYPES OF NEISSERIA GONORRHOEAE TO PRODUCE INFECTION AND AN INFLAMMATORY RESPONSE IN SUBCUTANEOUS PERFORATED PLASTIC CHAMBERS IN GUINEA-PIGS AND RABBITS , 1975 .

[5]  H. Farzadegan,et al.  Scanning electron microscopy and freeze-etching of gonorrhoeal urethral exudate. , 1975, The British journal of venereal diseases.

[6]  H. Smith,et al.  PENETRATION OF PENICILLIN INTO HUMAN PHAGOCYTES CONTAINING GONOCOCCI , 1975, The Lancet.

[7]  F. Ashton,et al.  Studies on gonococcal infection. I. Electron microscopic studies on phagocytosis of Neisseria gonorrhoeae by macrophages. , 1975, Japanese journal of microbiology.

[8]  R. Roberts,et al.  The interaction in vitro between human polymorphonuclear leukocytes and Neisseria gonorrhoeae cultivated in the chick embryo , 1975, The Journal of experimental medicine.

[9]  N. M. Ovcinnikov,et al.  Electron microscopy of gonococci in the urethral secretions of patients with gonorrhoea treated with penicillin and erythromycin. , 1974, The British journal of venereal diseases.

[10]  G. Mandell.  Interaction of intraleukocytic bacteria and antibiotics. , 1973, The Journal of clinical investigation.

[11]  D. Thomas,et al.  Interaction of Gonococci with Phagocytic Leukocytes from Men and Mice , 1973, Infection and immunity.

[12]  H. Smith,et al.  The chemical basis of the virulence of Brucella abortus. X. A surface virulence factor which facilitates intracellular growth of Brucella abortus in bovine phagocytes. , 1972, British journal of experimental pathology.

[13]  M. Ward,et al.  The fate of gonococci in polymorphonuclear leucocytes: an electron microscopic study of the natural disease. , 1972, British journal of experimental pathology.

[14]  N. M. Ovcinnikov,et al.  Electron microscope studies of gonococci in the urethral secretions of patients with gonorrhoea. , 1971, The British journal of venereal diseases.

[15]  P. J. Watt.  The fate of gonococci in polymorphonuclear leucocytes. , 1970, Journal of medical microbiology.

[16]  G. Youmans,et al.  Multiplication of Mycobacterium tuberculosis Within Normal and “Immune” Mouse Macrophages Cultivated With and Without Streptomycin , 1970, Infection and immunity.

[17]  H. Smith.  Biochemical challenge of microbial pathogenicity. , 1984, Bacteriological reviews.

[18]  D. S. Kellogg,et al.  Neisseria gonorrhoeae II. Colonial Variation and Pathogenicity During 35 Months In Vitro , 1968, Journal of bacteriology.

[19]  R. Good,et al.  Protection of Phagocytized Bacteria from the Killing Action of Antibiotics , 1966, Nature.

[20]  Douglas S. Kellogg,et al.  NEISSERIA GONORRHOEAE I , 1963, Journal of bacteriology.

[21]  J. D. Thayer,et al.  Failure of penicillin, chloramphenicol, erythromycin, and novobiocin to kill phagocytized gonococci in tissue. , 1956, Antibiotics annual.