Changes in cell proliferation due to environmental non-ionizing radiation: 2. Microwave radiation

Abstract Due to the use of mobile telephones, there is an increased exposure of the environment to weak radiofrequency (RF) electromagnetic fields, emitted by these devices. This study was undertaken to investigate if the microwave radiation from these fields will have a similar effect on cell proliferation as weak electromagnetic (ELF) fields. The field was generated by signal simulation of the Global System for Mobile communications (GSM) of 960 MHz. Cell cultures, growing in microtiter plates, were exposed in a specially constructed chamber, a Transverse Electromagnetic (TEM) cell. The Specific Absorption Rate (SAR) values for each cell well were calculated for this exposure system. Experiments were performed on cell cultures of transformed human epithelial amnion cells (AMA), which were exposed to 960 MHz microwave fields at three different power levels and three different exposure times, respectively. It was found that cell growth in the exposed cells was decreased in comparison to that in the control and sham exposed cells. Cell proliferation during the period following exposure varied not only with the various SAR levels, but also with the length of exposure time. On the other hand, repeated periods of exposure did not seem to change the effects. There was a general linear correlation between power level and growth change. However, the exposure time required to obtain the maximum effect was not the same for the various power levels. It turned out that at low power level, a maximum effect was first reached after a longer exposure time than at higher power level. A similar phenomenon was registered in the studies on ELF electromagnetic fields. Here, it was found that there was a linear correlation between the length of exposure time to obtain maximum effect and field strength.

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