Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig, and mouse.

The factors that control fat deposition in adipose tissues are poorly understood. It is known that visceral adipose tissues display a range of biochemical properties that distinguish them from adipose tissues of subcutaneous origin. However, we have little information on gene expression, either in relation to fat deposition or on interspecies variation in fat deposition. The first step in this study was to identify genes expressed in fat depot of cattle using the differential display RT-PCR method. Among the transcripts identified as having differential expression in the two adipose tissues were cell division cycle 42 homolog (CDC42), prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and kelch repeat and BTB domain containing 2 (Kbtbd2). In subsequent experiments, we determined the expression levels of these latter genes in the pig and in mice fed either a control or high-fat diet to compare the regulation of fat accumulation in other animal species. The levels of CDC42 and decorin mRNA were found to be higher in visceral adipose tissue than in subcutaneous adipose tissue in cattle, pig, and mice. However, the other genes studied did not show consistent expression patterns between the two tissues in cattle, pigs, and mice. Interestingly, all genes were upregulated in subcutaneous and/or visceral adipose tissues of mice fed the high-fat diet compared with the control diet. The data presented here extend our understanding of gene expression in fat depots and provide further proof that the mechanisms of fat accumulation differ significantly between animal species.

[1]  M. Bonnet,et al.  Nutritional status induces divergent variations of GLUT4 protein content, but not lipoprotein lipase activity, between adipose tissues and muscles in adult cattle , 2004, British Journal of Nutrition.

[2]  J. Wood,et al.  Restriction of dietary energy and protein induces molecular changes in young porcine skeletal muscles. , 2004, The Journal of nutrition.

[3]  D. Lahiri,et al.  Age‐related changes in murine CNS mRNA gene expression are modulated by dietary melatonin , 2004, Journal of pineal research.

[4]  J. Świerczyński,et al.  Potential role of high serum leptin concentration in age-related decrease of fatty acid synthase gene expression in rat white adipose tissue , 2004, Experimental Gerontology.

[5]  C. Zwieb,et al.  Characterization of a collection of deletion mutants at the 3′-end of 16S ribosomal RNA of Escherichia coli , 1986, Molecular and General Genetics MGG.

[6]  Soren Prag,et al.  Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals , 2003, BMC Bioinformatics.

[7]  M. Imagawa,et al.  Crucial Role of TCL/TC10βL, a Subfamily of Rho GTPase, in Adipocyte Differentiation* , 2003, The Journal of Biological Chemistry.

[8]  J. Olefsky,et al.  Cdc42 Is a Rho GTPase Family Member That Can Mediate Insulin Signaling to Glucose Transport in 3T3-L1 Adipocytes* , 2003, The Journal of Biological Chemistry.

[9]  E. Van Obberghen,et al.  Matrix Metalloproteinases Are Differentially Expressed in Adipose Tissue during Obesity and Modulate Adipocyte Differentiation* , 2003, The Journal of Biological Chemistry.

[10]  Byungkook Lee,et al.  Discovery of the breast cancer gene BASE using a molecular approach to enrich for genes encoding membrane and secreted proteins , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Ki-Choon Choi,et al.  Differential Expression of the Nonmuscle-type Cofilin Gene between Subcutaneous and Visceral Adipose Tissue , 2003, Bioscience, biotechnology, and biochemistry.

[12]  R. Schmidt-Kastner,et al.  DNA microarray analysis of cortical gene expression during early recirculation after focal brain ischemia in rat. , 2002, Brain research. Molecular brain research.

[13]  E. Lander,et al.  Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Xiaoming Li,et al.  A Human Mitochondrial GTP Binding Protein Related to tRNA Modification May Modulate Phenotypic Expression of the Deafness-Associated Mitochondrial 12S rRNA Mutation , 2002, Molecular and Cellular Biology.

[15]  Jinping Li,et al.  Gene expression profile of rat adipose tissue at the onset of high-fat-diet obesity. , 2002, American journal of physiology. Endocrinology and metabolism.

[16]  Ansuman Bagchi,et al.  Printed in U.S.A. Copyright © 2002 by The Endocrine Society Gene Expression Profile of Adipocyte Differentiation and Its Regulation by Peroxisome Proliferator-Activated , 2022 .

[17]  D. Gerhold,et al.  Gene expression profile of adipocyte differentiation and its regulation by peroxisome proliferator-activated receptor-gamma agonists. , 2002, Endocrinology.

[18]  C. Niesler,et al.  Adipose depot-specific expression of cIAP2 in human preadipocytes and modulation of expression by serum factors and TNFα , 2001, International Journal of Obesity.

[19]  M. Beylot,et al.  Effects of isoenergetic high-carbohydrate compared with high-fat diets on human cholesterol synthesis and expression of key regulatory genes of cholesterol metabolism. , 2001, The American journal of clinical nutrition.

[20]  B. Yandell,et al.  The expression of adipogenic genes is decreased in obesity and diabetes mellitus. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[21]  N. Welsh,et al.  Interleukin-1β Regulates Phospholipase D-1 Expression in Rat Pancreatic β-Cells. , 2000, Endocrinology.

[22]  N. Welsh,et al.  Interleukin-1beta regulates phospholipase D-1 expression in rat pancreatic beta-cells. , 2000, Endocrinology.

[23]  L. Cooley,et al.  The kelch repeat superfamily of proteins: propellers of cell function. , 2000, Trends in cell biology.

[24]  G. Privé,et al.  Crystal structure of the BTB domain from PLZF. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[25]  S. O’Rahilly,et al.  Depot-related gene expression in human subcutaneous and omental adipocytes. , 1998, Diabetes.

[26]  G. Fiermonte,et al.  Expression in Escherichia coli, Functional Characterization, and Tissue Distribution of Isoforms A and B of the Phosphate Carrier from Bovine Mitochondria* , 1998, The Journal of Biological Chemistry.

[27]  J. Vandekerckhove,et al.  Prefoldin, a Chaperone that Delivers Unfolded Proteins to Cytosolic Chaperonin , 1998, Cell.

[28]  L. Lönn,et al.  Assimilation of triglycerides in subcutaneous and intraabdominal adipose tissues in vivo in men: effects of testosterone. , 1996, The Journal of clinical endocrinology and metabolism.

[29]  D. Leprince,et al.  The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins. , 1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[30]  A. Kissebah,et al.  Regional adiposity and morbidity. , 1994, Physiological reviews.

[31]  F. Hilliou,et al.  Forskolin induces the reorganization of extracellular matrix fibronectin and cytoarchitecture in 3T3-F442A adipocytes: its effect on fibronectin gene expression. , 1994, Biochimica et biophysica acta.

[32]  J. Reich,et al.  Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). , 1993, Nucleic acids research.

[33]  L. Cooley,et al.  Kelch encodes a component of intercellular bridges in Drosophila egg chambers , 1993, Cell.

[34]  M. Jensen,et al.  Effects of body fat distribution on regional lipolysis in obesity. , 1991, The Journal of clinical investigation.

[35]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.