Fermentation of Opuntia stricta (Haw.) fruits for betalains concentration.

Fermentation of juice and homogenized fruits of Opuntia stricta fruits has been developed and optimized. The aim was to obtain the red food colorant betanin from prickly pear, at high concentration and low viscosity. Among three strains assayed, Saccharomyces cerevisiae var. bayanus AWRI 796 has been the optimum for this process. The optimum temperature value was found to be 35 degrees C for both sugar consumption and pigment preservation. After fermentation, biomass and residual vegetal tissue were discarded by centrifugation. Supernatant was concentrated under vacuum. Therefore, liquid concentrated betanin was obtained, with low viscosity and being sugar free. Besides, bioethanol was obtained as byproduct. Characteristics of the final product obtained were pH 3.41, 5.2 degrees Brix, 9.65 g/L betanin, color strength of 10.8, and viscosity of 52.5 cP. These values are better than obtained by other procedures.

[1]  G. Greif,et al.  Effect of fermentation on the composition of the red beet pigments , 1989 .

[2]  R. Carle,et al.  Phytochemical and nutritional significance of cactus pear , 2001 .

[3]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[4]  H. Corke,et al.  Effect of Postharvest Treatments on Amaranthus Betacyanin Degradation Evaluated by Visible/Near‐Infrared Spectroscopy , 2008 .

[5]  J. Fernández-López,et al.  Color properties and stability of betacyanins from Opuntia fruits. , 2003, Journal of agricultural and food chemistry.

[6]  J. Karovičová,et al.  Red beet pigment composition. Effects of fermentation by different strains of Saccharomyces cerevisiae , 1992 .

[7]  Seung-Heon Yoon,et al.  Specificity of yeast (Saccharomyces cerevisiae) in removing carbohydrates by fermentation. , 2003, Carbohydrate research.

[8]  U. Wissgott,et al.  Prospects for new natural food colorants , 1996 .

[9]  J. Fernández-López,et al.  The isolation and properties of a concentrated red-purple betacyanin food colourant from Opuntia stricta fruits , 2006 .

[10]  J. Fernández-López,et al.  Screening and mass-spectral confirmation of betalains in cactus pears , 2002 .

[11]  G. Sandoval,et al.  Effect of different fermentation conditions on the kinetic parameters and production of volatile compounds during the elaboration of a prickly pear distilled beverage , 2006, Journal of Industrial Microbiology and Biotechnology.

[12]  Mario Piattelll.  19 – The Betalains: Structure, Biosynthesis, and Chemical Taxonomy , 1981 .

[13]  F. García-Carmona,et al.  Characterization of the antiradical activity of betalains from Beta vulgaris L. roots , 1998 .

[14]  S. Laakso,et al.  Preparation of fermentable lingonberry juice through removal of benzoic acid by Saccharomyces cerevisiae yeast , 2003 .

[15]  D. Strack,et al.  Recent advances in betalain research. , 2003, Phytochemistry.

[16]  R. Carle,et al.  Color, betalain pattern, and antioxidant properties of cactus pear (Opuntia spp.) clones. , 2005, Journal of agricultural and food chemistry.

[17]  A. Polesello,et al.  High-performance liquid chromatographic analysis of the pigments of blood-red prickly pear (Opuntia ficus indica) , 1992 .

[18]  R. Carle,et al.  Effects of processing and storage on juice colour and betacyanin stability of purple pitaya (Hylocereus polyrhizus) juice , 2007 .

[19]  Oscar E. Bustos.  Alcoholic Beverage from Chilean Opuntia Ficus Indica , 1981 .

[20]  J. Fernández-López,et al.  Application of high-performance liquid chromatography to the characterization of the betalain pigments in prickly pear fruits. , 2001, Journal of chromatography. A.

[21]  L. Tesoriere,et al.  Antioxidant activities of sicilian prickly pear (Opuntia ficus indica) fruit extracts and reducing properties of its betalains: betanin and indicaxanthin. , 2002, Journal of agricultural and food chemistry.

[22]  E. Karababa,et al.  The effects of fermentation on the thermostability of the yellow-orange pigments extracted from cactus pear (Opuntia ficus-indica) , 2001 .

[23]  R. Reynoso,et al.  Stability of Betalain Pigments from a Cactacea Fruit , 1997 .

[24]  R. Carle,et al.  Betacyanins in fruits from red-purple pitaya, Hylocereus polyrhizus (Weber) Britton & Rose , 2002 .

[25]  H. Gottlieb,et al.  Betacyanins from vine cactus Hylocereus polyrhizus. , 2001, Phytochemistry.

[26]  H. Corke,et al.  Colorant properties and stability of Amaranthus Betacyanin pigments , 1998 .

[27]  S. Lee,et al.  Alcohol Fermentation of Opuntia ficus Fruit Juice , 2000 .

[28]  C. Sáenz.  Processing technologies: an alternative for cactus pear (Opuntia spp.) fruits and cladodes , 2000 .

[29]  R. Carle,et al.  Pigment pattern and expression of colour in fruits from different Hylocereus sp. genotypes , 2007 .