Selective isolation and enumeration of Gliocladium virens and G. roseum from soil

Selective media were developed for the isolation and enumeration of Gliocladium virens and G. roseum from soil. The basic antibiotic medium consisted of 3.0 g of glucose, 0.2 g of MgSO 4 .7H 2 O, 1.0 g of K 2 HPO 4 , 0.5 g of KCl, 1.0 g of NaNO 3 , 0.01 g of FeSO 4 .7H 2 O, 50 mg of chloramphenicol, 50 mg of rose bengal, 50 mg of streptomycin sulfate, 500μg of benomyl (50WP), 500 mg of sodium propionate, and 20 g of Bacto agar in 1 L of distilled water (...)