Tenderization of buffalo meat using plant proteases from Cucumis trigonus Roxb (Kachri) and Zingiber officinale roscoe (Ginger rhizome).

This study was conducted to develop a method for improving tenderness and overall qualities of tough buffalo meat using plant proteolytic enzymes from Cucumis trigonus Roxb (Kachri) and Zingiber officinale roscoe (Ginger rhizome). Their tenderizing efficacy was compared with the most popular enzyme papain. 3×3×3 cm chunks from Biceps femoris muscles of spent Murrah buffaloes (4-5 years age) were marinated with distilled water (control), 2% (w/w) powdered cucumis extract, 5% (w/v) ginger extract or 0.2% (w/w) papain for 48 h at 4 °C and subjected to various physico-chemical, histological and sensory evaluations. An increase (p<0.01) in collagen solubility, sarcoplasmic and myofibrillar protein solubility, and reduction (p<0.01) in shear force values were observed in all enzyme-treated samples compared to control. Electrophoretic pattern of muscle proteins also revealed extensive proteolysis and reduction in number of protein bands in all treated samples. Improvement (p<0.01) in flavor, juiciness, tenderness and overall acceptability scores were observed in all enzyme-treated samples compared to controls. Ginger extract-treated meat samples received better scores for appearance, flavor, tenderness and overall acceptability. From these results, it is shown that ginger and cucumis can be used as an effective alternative to papain.

[1]  F. B. Wardlaw,et al.  EFFECT OF POSTMORTEM MUSCLE CHANGES ON POULTRY MEAT LOAF PROPERTIES , 1973 .

[2]  J. Sahoo,et al.  Extension of shelf life of ground chevon during refrigerated storage by using ascorbic acid , 2000 .

[3]  N. Mahendrakar,et al.  Observations on some chemical and physical characteristics of buffalo meat. , 1994, Meat science.

[4]  Sanjod Kumar Mendiratta,et al.  Tenderizing and antioxidant effect of ginger extract on sheep meat , 2000 .

[5]  J. L. Forrest,et al.  Principles of Meat Science , 1994 .

[6]  R. E. Neuman,et al.  The determination of hydroxyproline. , 1950, The Journal of biological chemistry.

[7]  G. W. Snedecor.  Statistical Methods , 1964 .

[8]  J. T. Keeton.  Effects of Fat and NaCl/Phosphate Levels on the Chemical and Sensory Properties of Pork Patties , 1983 .

[9]  B M Naveena,et al.  Tenderisation of spent hen meat using ginger extract , 2001, British poultry science.

[10]  R. G. Kauffman,et al.  The relationship of sarcoplasmic and myofibrillar protein solubility to colour and water-holding capacity in porcine longissimus muscle. , 1999, Meat science.

[11]  Thomas E. Creighton,et al.  Protein structure : a practical approach , 1997 .

[12]  K. V. Gilbert,et al.  Studies in Meat Tenderness. , 1968 .

[13]  N. Mahendrakar,et al.  Studies on influence of age of sheep and post-mortem carcass conditioning treatments on muscular collagen content and its thermolability , 1989 .

[14]  H. Tuma,et al.  Relationship of Fiber Diameter to Tenderness and Meatiness as Influenced by Bovine Age , 1962 .

[15]  D. L. Harrison,et al.  Collagen Determination, Comparison of Enzyme and Waring Blendor Methods for Determination of Collagen in Beef , 1960 .

[16]  M. Inouye,et al.  The effect of amino acid deletion in subtilisin E, based on structural comparison with a microbial alkaline elastase, on its substrate specificity and catalysis. , 1992, Journal of Biochemistry (Tokyo).

[17]  E. E. Rice,et al.  DEGRADATION OF VARIOUS MEAT FRACTIONS BY TENDERIZING ENZYMES , 1970 .

[18]  J. F. Price,et al.  PROTEOLYTIC ACTIVITY OF Pseudomonas perolens AND EFFECTS ON PORCINE MUSCLE , 1974 .

[19]  C. E. Allen,et al.  GINGER RHIZOME: A NEW SOURCE OF PROTEOLYTIC ENZYME , 1973 .

[20]  B. Amla,et al.  Effect of Lactic Acid, Ginger Extract and Sodium Chloride on Electrophoretic Pattern of Buffalo Muscle Proteins , 1995 .

[21]  P. V. Harris,et al.  INFLUENCE OF pH AND FIBER CONTRACTION STATE UPON FACTORS AFFECTING THE TENDERNESS OF BOVINE MUSCLE , 1973 .

[22]  E. Huff-Lonergan,et al.  Proteolysis of specific muscle structural proteins by mu-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle. , 1996, Journal of animal science.

[23]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.