A microfluidic platform to study pathogen-host interactions at single cell level
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[1] Jerome P Ferrance,et al. Microfluidic-based DNA purification in a two-stage, dual-phase microchip containing a reversed-phase and a photopolymerized monolith. , 2007, Analytical chemistry.
[2] Timothy B. Stockwell,et al. Nanoliter Reactors Improve Multiple Displacement Amplification of Genomes from Single Cells , 2007, PLoS genetics.
[3] R. Gibson,et al. Pathophysiology and management of pulmonary infections in cystic fibrosis. , 2003, American journal of respiratory and critical care medicine.
[4] T. Pitt,et al. Role of lipopolysaccharide in virulence of Pseudomonas aeruginosa , 1984, Infection and immunity.
[5] T. Mok,et al. Single-Molecule Detection of Epidermal Growth Factor Receptor Mutations in Plasma by Microfluidics Digital PCR in Non–Small Cell Lung Cancer Patients , 2009, Clinical Cancer Research.
[6] Vincent Studer,et al. A nanoliter-scale nucleic acid processor with parallel architecture , 2004, Nature Biotechnology.
[7] Robin H. Liu,et al. Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection. , 2004, Analytical chemistry.
[8] Ujwal S. Setlur,et al. Development of an automated DNA purification module using a micro-fabricated pillar chip. , 2008, The Analyst.
[9] H. Maeda,et al. Isolation and characterization of nucleases from a clinical isolate of Serratia marcescens kums 3958. , 1983, Journal of Biochemistry (Tokyo).
[10] Varun Reddy,et al. Interfacial stabilization of organic-aqueous two-phase microflows for a miniaturized DNA extraction module. , 2005, Journal of colloid and interface science.
[11] R. Jaenisch,et al. Microfluidic Control of Cell Pairing and Fusion , 2009, Nature Methods.
[12] R. Phadke,et al. Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studies. , 2003, Journal of microbiological methods.
[13] S. Quake,et al. Monolithic microfabricated valves and pumps by multilayer soft lithography. , 2000, Science.
[14] Chaoyong James Yang,et al. High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets. , 2008, Analytical chemistry.
[15] Luke P. Lee,et al. Dynamic single cell culture array. , 2006, Lab on a chip.
[16] Dieter Klein,et al. Quantification using real-time PCR technology : applications and limitations , 2002 .
[17] T. F. Smith,et al. Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing , 2006, Clinical Microbiology Reviews.
[18] A. Folch,et al. Large-scale single-cell trapping and imaging using microwell arrays. , 2005, Analytical chemistry.
[19] Stephen R Quake,et al. Parallel picoliter rt-PCR assays using microfluidics. , 2006, Analytical chemistry.
[20] Y. Murakami,et al. Development of a microchamber array for picoliter PCR. , 2001, Analytical chemistry.
[21] David J. Evans,et al. Pseudomonas aeruginosa Invasion and Cytotoxicity Are Independent Events, Both of Which Involve Protein Tyrosine Kinase Activity , 1998, Infection and Immunity.
[22] H. Moriguchi,et al. An agar-based on-chip neural-cell-cultivation system for stepwise control of network pattern generation during cultivation , 2004 .
[23] Z. Karim,et al. Acid pH increases the stability of BSC1/NKCC2 mRNA in the medullary thick ascending limb. , 2003, Journal of the American Society of Nephrology : JASN.
[24] Elisabetta Delibato,et al. Evaluation of DNA Extraction Methods for Use in Combination with SYBR Green I Real-Time PCR To Detect Salmonella enterica Serotype Enteritidis in Poultry , 2003, Applied and Environmental Microbiology.
[25] Numrin Thaitrong,et al. Integrated microfluidic bioprocessor for single-cell gene expression analysis , 2008, Proceedings of the National Academy of Sciences.
[26] Andrew D Griffiths,et al. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. , 2009, Analytical chemistry.
[27] Brian J. Mailloux,et al. Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Environments , 2000, Applied and Environmental Microbiology.
[28] Peter J. Maimonis,et al. Affinity and size capture of circulating tumor cells: A platform for increased sensitivity , 2010 .
[29] Stephen R. Quake,et al. Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria , 2006, Science.
[30] S. Quake,et al. An Integrated Microfabricated Cell Sorter , 2022 .
[31] Scott A. Rifkin,et al. Imaging individual mRNA molecules using multiple singly labeled probes , 2008, Nature Methods.
[32] Alessandra Ghiani,et al. Resolution of Viable and Membrane-Compromised Bacteria in Freshwater and Marine Waters Based on Analytical Flow Cytometry and Nucleic Acid Double Staining , 2001, Applied and Environmental Microbiology.
[33] Helene Andersson-Svahn,et al. A microwell array device with integrated microfluidic components for enhanced single‐cell analysis , 2009, Electrophoresis.
[34] D. Weitz,et al. Geometrically mediated breakup of drops in microfluidic devices. , 2003, Physical review letters.
[35] Real-time quantitative polymerase chain reaction analysis of mitochondrial DNA point mutation. , 2006, Methods in molecular biology.
[36] S. Lory,et al. Interaction of Pseudomonas aeruginosa with A549 pneumocyte cells , 1991, Infection and immunity.
[37] Christopher R. Lowe,et al. Silicon microchambers for DNA amplification , 1998 .
[38] K. A. Wolfe,et al. Microchip-based purification of DNA from biological samples. , 2003, Analytical chemistry.
[39] R. Zare,et al. Chemical cytometry on a picoliter-scale integrated microfluidic chip. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[40] D J Harrison,et al. mRNA isolation in a microfluidic device for eventual integration of cDNA library construction. , 2000, The Analyst.
[41] Gwo-Bin Lee,et al. Membrane-activated microfluidic rotary devices for pumping and mixing , 2007, Biomedical microdevices.
[42] M. Kew,et al. Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit, GeneReleaser, and the phenol-chloroform method , 1996, Journal of clinical microbiology.
[43] M. Goldsworthy. Gene expression of Pseudomonas aeruginosa and MRSA within a catheter-associated urinary tract infection biofilm model , 2008 .
[44] D. Durand,et al. A novel integrable microvalve for refreshable Braille display system , 2003 .
[45] S. Klaschik,et al. Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis , 2004, Journal of Clinical Microbiology.
[46] C. Di Serio,et al. Pseudomonas aeruginosa microevolution during cystic fibrosis lung infection establishes clones with adapted virulence. , 2009, American journal of respiratory and critical care medicine.
[47] C. S. Chen,et al. Geometric control of cell life and death. , 1997, Science.
[48] M. A. Northrup,et al. A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers. , 1998, Analytical chemistry.
[49] Wan-Chi Lee,et al. An integrated microfluidic system using magnetic beads for virus detection. , 2008, Diagnostic microbiology and infectious disease.
[50] J. Zahn,et al. Two phase micromixing and analysis using electrohydrodynamic instabilities , 2006 .
[51] T. Pistole,et al. OmpD but not OmpC is involved in adherence of Salmonella enterica serovar typhimurium to human cells. , 2004, Canadian journal of microbiology.
[52] P. Poole. The role of hydration in lysozyme structure and activity: relevance in protein engineering and design , 1994 .
[53] D. Meldrum,et al. Real-time PCR of single bacterial cells on an array of adhering droplets. , 2011, Lab on a chip.
[54] Hiroyuki Kishi,et al. Single lymphocyte analysis with a microwell array chip , 2007, Cytometry. Part A : the journal of the International Society for Analytical Cytology.
[55] Mehmet Toner,et al. Controlled encapsulation of single-cells into monodisperse picolitre drops. , 2008, Lab on a chip.
[56] Ronald R. Breaker,et al. Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group , 1999 .
[57] Saeed A. Khan,et al. A simple and efficient Triton X-100 boiling and chloroform extraction method of RNA isolation from Gram-positive and Gram-negative bacteria. , 2003, FEMS microbiology letters.
[58] R. C. Davies,et al. The dependence of lysozyme activity on pH and ionic strength. , 1969, Biochimica et biophysica acta.
[59] C. Hauck,et al. Microscopic quantification of bacterial invasion by a novel antibody-independent staining method. , 2004, Journal of microbiological methods.
[60] S. Bonifacio,et al. Pseudomonas pneumonia in infants: an autopsy study. , 2003, Human pathology.
[61] G. Rogers,et al. Characterization of Bacterial Community Diversity in Cystic Fibrosis Lung Infections by Use of 16S Ribosomal DNA Terminal Restriction Fragment Length Polymorphism Profiling , 2004, Journal of Clinical Microbiology.
[62] C. Batt,et al. Nucleic acid purification using microfabricated silicon structures. , 2003, Biosensors & bioelectronics.
[63] M. Kolak,et al. Molecular typing of the bacterial flora in sputum of cystic fibrosis patients. , 2003, International journal of medical microbiology : IJMM.
[64] A. Hauser. The type III secretion system of Pseudomonas aeruginosa: infection by injection , 2009, Nature Reviews Microbiology.
[65] Stephen R Quake,et al. Microfluidic single-cell mRNA isolation and analysis. , 2006, Analytical chemistry.
[66] Makoto Ishida,et al. A MEMS microvalve with PDMS diaphragm and two-chamber configuration of thermo-pneumatic actuator for integrated blood test system on silicon , 2005 .
[67] G. Bellon,et al. Localization of Staphylococcus aureus in infected airways of patients with cystic fibrosis and in a cell culture model of S. aureus adherence. , 1998, American journal of respiratory cell and molecular biology.
[68] L. Burrows,et al. Genetics of O-Antigen Biosynthesis inPseudomonas aeruginosa , 1999, Microbiology and Molecular Biology Reviews.
[69] J. Lacroix,et al. PCR-based technique for the detection of bacteria in semen and urine , 1996 .
[70] C. Hart,et al. Increased morbidity associated with chronic infection by an epidemic Pseudomonas aeruginosa strain in CF patients , 2004, Thorax.
[71] M. Donnenberg,et al. Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFP), EspA filaments and intimin. , 2004, Microbiology.
[72] Werner Karl Schomburg,et al. An electrostatically actuated polymer microvalve equipped with a movable membrane electrode , 1997 .
[73] Bacterial Diversity in Cases of Lung Infection in Cystic Fibrosis Patients: 16S Ribosomal DNA (rDNA) Length Heterogeneity PCR and 16S rDNA Terminal Restriction Fragment Length Polymorphism Profiling , 2003, Journal of Clinical Microbiology.
[74] P. Chomczyński,et al. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on , 2006, Nature Protocols.
[75] Hai-Qing Gong,et al. Rapid distribution of a liquid column into a matrix of nanoliter wells for parallel real-time quantitative PCR , 2009 .
[76] E. Fletcher,et al. Pseudomonas aeruginosa invades corneal epithelial cells during experimental infection , 1994, Infection and immunity.
[77] Kwai Peng Chan,et al. Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma. , 2002, Journal of virological methods.
[78] Hans Lehrach,et al. Quantitative PCR based expression analysis on a nanoliter scale using polymer nano-well chips , 2007, Biomedical microdevices.
[79] V. N. Perera,et al. Evaluation of eight RNA isolation methods for transcriptional analysis in Campylobacter jejuni. , 2007, Journal of microbiological methods.
[80] J. Emerson,et al. Impact of Pseudomonas and Staphylococcus infection on inflammation and clinical status in young children with cystic fibrosis. , 2009, The Journal of pediatrics.
[81] J. Rossier,et al. Integrating whole transcriptome assays on a lab-on-a-chip for single cell gene profiling. , 2008, Lab on a chip.
[82] W. Strober. Trypan blue exclusion test of cell viability. , 2001, Current protocols in immunology.
[83] W. Grajek,et al. Evaluation of quantitative PCR measurement of bacterial colonization of epithelial cells. , 2010, Polish journal of microbiology.
[84] Gwo-Bin Lee,et al. Magnetic-bead-based microfluidic system for ribonucleic acid extraction and reverse transcription processes , 2009, Biomedical microdevices.
[85] Tomoharu Kajiyama,et al. Quantitative analysis of gene expression in a single cell by qPCR , 2009, Nature Methods.
[86] D. Chiu,et al. Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets. , 2005, Analytical chemistry.
[87] G. Whitesides,et al. Rapid prototyping of microfluidic switches in poly(dimethyl siloxane) and their actuation by electro-osmotic flow , 1999 .
[88] M. Magnani,et al. Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods , 2007 .
[89] M. Bowen,et al. Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples. , 2009, Journal of microbiological methods.
[90] Jerome P Ferrance,et al. DNA extraction using a tetramethyl orthosilicate-grafted photopolymerized monolithic solid phase. , 2006, Analytical chemistry.
[91] Julie A. Wu,et al. Lysostaphin Disrupts Staphylococcus aureus and Staphylococcus epidermidis Biofilms on Artificial Surfaces , 2003, Antimicrobial Agents and Chemotherapy.
[92] C. Sze,et al. Dual fluorescence system for flow cytometric analysis of Escherichia coli transcriptional response in multi-species context. , 2009, Journal of microbiological methods.
[93] B. P. Kaistha,et al. Systematic comparison of RNA extraction techniques from frozen and fresh lung tissues: checkpoint towards gene expression studies , 2009, Diagnostic pathology.
[94] P Belgrader,et al. A minisonicator to rapidly disrupt bacterial spores for DNA analysis. , 1999, Analytical chemistry.
[95] Jerome P Ferrance,et al. Microchip-based macroporous silica sol-gel monolith for efficient isolation of DNA from clinical samples. , 2006, Analytical chemistry.
[96] M. Lonetto,et al. Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action. , 2000, Gene.
[97] Beatrice Vitali,et al. Real-time PCR quantification of bacterial adhesion to Caco-2 cells: competition between bifidobacteria and enteropathogens. , 2005, Research in microbiology.
[98] Helene Andersson-Svahn,et al. Overview of single-cell analyses: microdevices and applications. , 2010, Lab on a chip.
[99] Kevin D Dorfman,et al. Droplet fusion by alternating current (AC) field electrocoalescence in microchannels , 2005, Electrophoresis.
[100] W. Tan,et al. Comparison of different methods of total RNA extraction for viral detection in sputum. , 2001, Journal of virological methods.
[101] Kensall D. Wise,et al. A high-flow thermopneumatic microvalve with improved efficiency and integrated state sensing , 2003 .
[102] Sung-Dong Yang,et al. Experimental Demonstration and Numerical Simulation of Organic-Aqueous Liquid Extraction Enhanced by Droplet Formation in a Microfluidic Channel , 2006 .
[103] A. Azghani,et al. Virulence Factors from Pseudomonas aeruginosa Increase Lung Epithelial Permeability , 2000, Lung.
[104] M. Preobrazhenskaya,et al. Structures of Staphylococcus aureus cell-wall complexes with vancomycin, eremomycin, and chloroeremomycin derivatives by 13C{19F} and 15N{19F} rotational-echo double resonance. , 2006, Biochemistry.
[105] K. A. Wolfe,et al. Toward a microchip‐based solid‐phase extraction method for isolation of nucleic acids , 2002, Electrophoresis.
[106] M. Salimans,et al. Rapid and simple method for purification of nucleic acids , 1990, Journal of clinical microbiology.
[107] J. Yadav,et al. Development of a Single-Tube, Cell Lysis-Based, Genus-Specific PCR Method for Rapid Identification of Mycobacteria: Optimization of Cell Lysis, PCR Primers and Conditions, and Restriction Pattern Analysis , 2004, Journal of Clinical Microbiology.
[108] Gerald B. Pier,et al. Lung Infections Associated with Cystic Fibrosis , 2002, Clinical Microbiology Reviews.
[109] K. Manning,et al. Isolation of nucleic acids from plants by differential solvent precipitation. , 1991, Analytical biochemistry.
[110] T. Asahara,et al. Sensitive Quantitative Detection of Commensal Bacteria by rRNA-Targeted Reverse Transcription-PCR , 2006, Applied and Environmental Microbiology.
[111] Richard Novak,et al. High-performance single cell genetic analysis using microfluidic emulsion generator arrays. , 2010, Analytical chemistry.
[112] C. Klapperich,et al. Cell lysis and DNA extraction of gram-positive and gram-negative bacteria from whole blood in a disposable microfluidic chip. , 2009, Lab on a chip.
[113] G. Felsenfeld,et al. Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci , 2001, The EMBO journal.
[114] Samuel Aparicio,et al. High-throughput microfluidic single-cell RT-qPCR , 2011, Proceedings of the National Academy of Sciences.
[115] O. Nanassy,et al. Capture of genomic DNA on glass microscope slides. , 2007, Analytical biochemistry.
[116] N. Blin,et al. A general method for isolation of high molecular weight DNA from eukaryotes. , 1976, Nucleic acids research.
[117] Dong-Chul Han,et al. PDMS-based micro PCR chip with Parylene coating , 2003 .
[118] Axel Scherer,et al. A microfluidic processor for gene expression profiling of single human embryonic stem cells. , 2008, Lab on a chip.
[119] Catalin C. Barbacioru,et al. mRNA-Seq whole-transcriptome analysis of a single cell , 2009, Nature Methods.
[120] M. Surette,et al. A polymicrobial perspective of pulmonary infections exposes an enigmatic pathogen in cystic fibrosis patients , 2008, Proceedings of the National Academy of Sciences.
[121] Stephen R Quake,et al. Solving the "world-to-chip" interface problem with a microfluidic matrix. , 2003, Analytical chemistry.