β-Carotene Probes Energy Transfer Pathway in Photosystem II Core Complex.

The dynamics of intact photosystem II core complex (PSII-CC) has been investigated extensively to elucidate its excellent photofunction. However, it is significantly difficult to observe the primary photosynthetic processes in PSII-CC, because vast number of chlorophylls (Chl) in the core complex show similar spectral features. In the present work, dynamics of the energy transfer (ET) from β-carotene (Bcr) in intact PSII-CC followed by charge separation (CS) at reaction center (RC) with different excitation wavelengths were compared. Upon excitation at 510 nm, which selectively excite Bcr (Bcr651) inside the D1-D2 RC, pheophytin anion absorption band appeared within 9.6 ps. On the other hand, upon excitation at 490 nm, mainly exciting unspecified Bcr in the antenna complex, the anion band appeared after 20 ps. These excitation wavelength dependence experiments revealed a new ET pathway of PSII-CC which indicates that the initial CS of PSII-CC is limited by ET to the RC.

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