Towards a high-throughput label-free detection system combining localized-surface plasmon resonance and microfluidics.

This work reports an integrated platform combining localized-surface plasmon resonance (LSPR) and microfluidic chips to carry out multiplexed and label-free protein analysis. We fabricated an optical bench to enable detection using only a laboratory UV-Vis spectrophotometer. This assay not only improves throughput, but also allows quantitative analysis.

[1]  C. Mirkin,et al.  Protein Nanoarrays Generated By Dip-Pen Nanolithography , 2002, Science.

[2]  Shangjr Gwo,et al.  The facile fabrication of tunable plasmonic gold nanostructure arrays using microwave plasma. , 2010, Nanotechnology.

[3]  Kang Sun,et al.  Recent developments employing new materials for readout in lab-on-a-chip , 2010 .

[4]  E. Grabbe Total internal reflection fluorescence with energy transfer : a method for analyzing IgG adsorption on nylon thin films , 1993 .

[5]  E. Hutter,et al.  Exploitation of Localized Surface Plasmon Resonance , 2004 .

[6]  Kuan-Jiuh Lin,et al.  High sensitivity and selectivity of human antibody attachment at the interstices between substrate-bound gold nanoparticles. , 2011, Chemical communications.

[7]  N. Sugimoto,et al.  Measurements of the binding of a large protein using a substrate density-controlled DNA chip. , 2011, Analytical chemistry.

[8]  Tatsuro Endo,et al.  A localized surface plasmon resonance based immunosensor for the detection of casein in milk , 2007 .

[9]  Jean-Francois Masson,et al.  Advances in surface plasmon resonance sensing with nanoparticles and thin films: nanomaterials, surface chemistry, and hybrid plasmonic techniques. , 2011, Analytical chemistry.

[10]  Yunyan Xie,et al.  Using azobenzene-embedded self-assembled monolayers to photochemically control cell adhesion reversibly. , 2009, Angewandte Chemie.

[11]  Masato Saito,et al.  A Microfluidic Chip Based on Localized Surface Plasmon Resonance for Real-Time Monitoring of Antigen-Antibody Reactions , 2008 .

[12]  Xingyu Jiang,et al.  Microfluidic Western blot. , 2010, Analytical chemistry.

[13]  Liesbet Lagae,et al.  Localized surface plasmon resonance biosensor integrated with microfluidic chip , 2009, Biomedical microdevices.

[14]  Jeffrey N. Anker,et al.  Biosensing with plasmonic nanosensors. , 2008, Nature materials.

[15]  M. Komiyama,et al.  Single-molecule accommodation of streptavidin in nanometer-scale wells formed in DNA nanostructures. , 2008, Nucleic acids symposium series.

[16]  R. V. Van Duyne,et al.  Localized surface plasmon resonance spectroscopy and sensing. , 2007, Annual review of physical chemistry.

[17]  Dong Wang,et al.  Using self-polymerized dopamine to modify the antifouling property of oligo(ethylene glycol) self-assembled monolayers and its application in cell patterning. , 2011, Langmuir : the ACS journal of surfaces and colloids.