EFFECT OF MICROBIAL GROWTH UPON SARCOPLASMIC AND UREA‐SOLUBLE PROTEINS FROM MUSCLE

SUMMARY– A comparison of starch gel patterns of sarcoplasmic proteins from aseptic and inoculated porcine and rabbit muscles after storage for 0, 8 and 20 days at 10°C indicated that different microorganisms preferentially utilized specific proteins. Pseudomonas fragi showed the greatest amount of proteolytic activity upon the sarcoplasmic fraction, causing extensive breakdown in both rabbit and porcine muscle. Leuconostoc mesenteroides caused extensive alteration in the sarcoplasmic proteins of rabbit muscle, but had less effect upon porcine muscle. Pediococcus cerevisiae exhibited similar action to L. mesenteroides upon rabbit muscle sarcoplasmic proteins, but had no effect upon pig muscle. Micrococcus luteus showed only minor breakdown of rabbit muscle sarcoplasmic proteins, and had no action upon porcine muscle. Both P. fragi and P. cerevisiae caused considerable breakdown of the urea-soluble proteins in pig muscle and to a lesser extent in rabbit muscle. Neither M. luteus nor L. mesenteroides exerted any measurable proteolytic effect upon the urea-soluble proteins. Possible implications concerning meat spoilage are discussed.

[1]  E. E. Burns,et al.  Starch characteristics of selected grain sorghums as related to human foods , 1970 .

[2]  R. Scopes.  Methods for starch-gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscles of a variety of species. , 1968, The Biochemical journal.

[3]  J. M. Jay,et al.  Fate of free amino acids and nucleotides in spoiling beef. , 1967, Applied microbiology.

[4]  J. M. Jay.  Nature, characteristics, and proteolytic properties of beef spoilage bacteria at low and high temperatures. , 1967, Applied microbiology.

[5]  R. Fischer,et al.  Acrylamide Gel Disc Electrophoretic Patterns and Extractability of Chicken Breast Muscle Proteins During Post‐Mortem Aging , 1966 .

[6]  B. Davis.  DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS * , 1964, Annals of the New York Academy of Sciences.

[7]  R. Scopes.  The influence of post-mortem conditions on the solubilities of muscle proteins. , 1964, The Biochemical journal.

[8]  A. M. Pearson,et al.  The Relationship of Some Intracellular Protein Characteristics to Beef Muscle Tenderness a,b , 1963 .

[9]  J. A. Alford.  EFFECT OF INCUBATION TEMPERATURE ON BIOCHEMICAL TESTS IN THE GENERA PSEUDOMONAS AND ACHROMOBACTER , 1960, Journal of bacteriology.

[10]  S. Jacobs.  Determination of Nitrogen in Proteins by means of Indanetrione Hydrate , 1959, Nature.

[11]  C. Got,et al.  Detection of Hæmoglobin, Hæmoglobin–Haptoglobin Complexes and other Substances with Peroxidase Activity after Zone Electrophoresis , 1958, Nature.

[12]  A. D. Brown,et al.  THE TAXONOMY OF THE PSYCHROPHILIC MEAT-SPOILAGE BACTERIA: A REASSESSMENT , 1958 .

[13]  J. Ayres.  TEMPERATURE RELATIONSHIPS AND SOME OTHER CHARACTERISTICS OF THE MICROBIAL FLORA DEVELOPING ON REFRIGERATED BEEF , 1960 .