Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased (P < 0.001). Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 (P < 0.001). Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 (P < 0.001). In case-2, serum creatinine was significantly correlated with SPA only (P < 0.001). Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage.

[1]  A. Verma,et al.  Serum Prolidase Activity, Oxidant and Antioxidant Status in Nonulcer Dyspepsia and Healthy Volunteers , 2013, ISRN biochemistry.

[2]  R. Prasad,et al.  Oxidative stress in children with bacterial meningitis. , 2013, Journal of tropical pediatrics.

[3]  E. Uzar,et al.  Serum prolidase activity and oxidative status in patients with diabetic neuropathy , 2012, Neurological Sciences.

[4]  H. Çelik,et al.  The association of serum prolidase activity and erectile dysfunction. , 2010, Journal of andrology.

[5]  C. Schmid,et al.  A new equation to estimate glomerular filtration rate. , 2009, Annals of internal medicine.

[6]  A. Surazynski,et al.  Prolidase-dependent regulation of collagen biosynthesis , 2008, Amino Acids.

[7]  M. Salifu,et al.  Oxidative stress, glucose metabolism, and the prevention of type 2 diabetes: pathophysiological insights. , 2007, Antioxidants & redox signaling.

[8]  R. Robertson,et al.  Diabetes, glucose toxicity, and oxidative stress: A case of double jeopardy for the pancreatic islet beta cell. , 2006, Free radical biology & medicine.

[9]  Jean-Paul Cristol,et al.  Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes. , 2006, JAMA.

[10]  O. Erel,et al.  A new automated colorimetric method for measuring total oxidant status. , 2005, Clinical biochemistry.

[11]  T. Hosoya,et al.  Oxidative stress is enhanced in correlation with renal dysfunction: examination with the redox state of albumin. , 2004, Kidney international.

[12]  O. Erel,et al.  A novel automated method to measure total antioxidant response against potent free radical reactions. , 2004, Clinical biochemistry.

[13]  R. Robertson,et al.  β-Cell Glucose Toxicity, Lipotoxicity, and Chronic Oxidative Stress in Type 2 Diabetes , 2004 .

[14]  Kuo-Chin Huang,et al.  Reduced hemodialysis-induced oxidative stress in end-stage renal disease patients by electrolyzed reduced water. , 2003, Kidney international.

[15]  A. Erbaǧci,et al.  Serum prolidase activity as a marker of osteoporosis in type 2 diabetes mellitus. , 2002, Clinical biochemistry.

[16]  M. Cooper,et al.  The tubulointerstitium in progressive diabetic kidney disease: more than an aftermath of glomerular injury? , 1999, Kidney international.

[17]  J. Phang,et al.  Prolidase activity in fibroblasts is regulated by interaction of extracellular matrix with cell surface integrin receptors , 1997, Journal of cellular biochemistry.

[18]  T. Schacker,et al.  Clinical and Epidemiologic Features of Primary HIV Infection , 1996, Annals of Internal Medicine.

[19]  I. Myara,et al.  Plasma prolidase activity: a possible index of collagen catabolism in chronic liver disease. , 1984, Clinical chemistry.

[20]  C. Mogensen,et al.  The Stages in Diabetic Renal Disease: With Emphasis on the Stage of Incipient Diabetic Nephropathy , 1983, Diabetes.

[21]  M. Bergmann,et al.  A NEW TYPE OF ENZYME IN THE INTESTINAL TRACT. , 1936, Science.

[22]  Richard Barnett.  Diabetes , 1904, The Lancet.

[23]  G. Nath,et al.  Mucosal IgA & IL-1β in Helicobacter pylori Infection , 2012, Indian Journal of Clinical Biochemistry.

[24]  W. Marsden.  I and J , 2012 .

[25]  J. Pincemail,et al.  [Oxidative stress]. , 2007, Revue medicale de Liege.

[26]  M. Schiffer,et al.  Glucose-induced reactive oxygen species cause apoptosis of podocytes and podocyte depletion at the onset of diabetic nephropathy. , 2006, Diabetes.

[27]  J. Watkins,et al.  Diabetes, oxidative stress, and antioxidants: A review , 2003, Journal of biochemical and molecular toxicology.

[28]  S. De Flora,et al.  Oltipraz chemoprevention trial in Qidong, Jiangsu Province, People's Republic of China , 1997, Journal of cellular biochemistry. Supplement.

[29]  G. Cetta,et al.  Prolidase deficiency: biochemical study of erythrocyte and skin fibroblast prolidase activity in Italian patients. , 1994, Haematologica.

[30]  E. Joslin,et al.  Joslin's Diabetes Mellitus , 1971 .

[31]  H. Hanson.  Proteolytic enzymes. , 1962, Experimental eye research.