论文信息 - Chemiluminescence assay of alkaline phosphatase using cortisol-21 -phosphate as substrate and its application to enzyme immunoassays

Chemiluminescence assay of alkaline phosphatase using cortisol-21 -phosphate as substrate and its application to enzyme immunoassays

Abstract A method is described for the highly sensitive chemiluminescence (CL) assay for alkaline phosphatase (ALP) using cortisol-21-phosphate (cortisol-21-P) as substrate and lucigenin as CL reagent; it is applied to an enzyme immunoassay (EIA) for human chorionic gonadotropin (hCG), human growth hormone (hGH), α-fetoprotein (AFP) and estradiol (E 2 ). The detection limit for ALP was 8.75 × 10 −20 mol/assay, and the average coefficient of variation ( n = 11) over the calibration range (3.55 × 10 −15 –7.1 × 10 −10 mol l −1 ) was 6.4%. In EIA for hCG, hGH, AFP and E 2 , the detection limits were 0.008 mIU ml −1 . 10, 40 and 0.25 pg ml −1 , respectively.

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