A Step Toward Learning to Control Tens of Optically Actuated Microrobots in Three Dimensions

Automated manipulation of a large number of independently controlled microrobots in three dimensions can potentially lead to a major breakthrough in the formation of functional microstructures, both engineering and biological, in a scalable and reliable manner. In this paper, we provide the first foundational step toward realizing this objective by developing a reinforcement learning-based model predictive controller. Our controller (approximately) optimizes the trajectories of the microrobots through importance sampling, while ensuring that the trajectories do not cause collisions with workspace obstacles, such as other randomly dispersed microobjects or already-formed microstructures. Simulation experiments with tens of microspheres, actuated using holographic optical traps, demonstrate the feasibility and usefulness of our method.

[1]  John D. Hunter,et al.  Matplotlib: A 2D Graphics Environment , 2007, Computing in Science & Engineering.

[2]  Satyandra K. Gupta,et al.  Research in Automated Planning and Control for Micromanipulation , 2013, IEEE Transactions on Automation Science and Engineering.

[3]  Pal Ormos,et al.  Orientation of flat particles in optical tweezers by linearly polarized light. , 2003, Optics express.

[4]  Xiang Li,et al.  Observer-Based Optical Manipulation of Biological Cells With Robotic Tweezers , 2014, IEEE Transactions on Robotics.

[5]  Wolfgang Wende,et al.  STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA , 2013, Nature Methods.

[6]  D. Grier A revolution in optical manipulation , 2003, Nature.

[7]  Wenqi Hu,et al.  Interactive actuation of multiple opto-thermocapillary flow-addressed bubble microrobots , 2014, Robotics and biomimetics.

[8]  Salvador Pané,et al.  Recent developments in magnetically driven micro- and nanorobots , 2017 .

[9]  Can Wang,et al.  Transportation of Multiple Biological Cells Through Saturation-Controlled Optical Tweezers In Crowded Microenvironments , 2016, IEEE/ASME Transactions on Mechatronics.

[10]  Keshav Rajasekaran,et al.  Imaging-guided collision-free transport of multiple optically trapped beads , 2017, 2017 International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS).

[11]  Stefan Schaal,et al.  Policy Gradient Methods for Robotics , 2006, 2006 IEEE/RSJ International Conference on Intelligent Robots and Systems.

[12]  Akbar Samadi,et al.  Evaluating the toxic effect of an antimicrobial agent on single bacterial cells with optical tweezers. , 2015, Biomedical optics express.

[13]  L. Oddershede,et al.  Expanding the optical trapping range of gold nanoparticles. , 2005, Nano letters.

[14]  Giovanni Volpe,et al.  Optical trapping and manipulation of nanostructures. , 2013, Nature nanotechnology.

[15]  Jiashu Sun,et al.  Microfluidics for manipulating cells. , 2013, Small.

[16]  Markus Böl,et al.  Micro-Gripper: A new concept for a monolithic single-cell manipulation device , 2015 .

[17]  Zhidong Wang,et al.  Cooperative Micromanipulation Using the Independent Actuation of Fifty Microrobots in Parallel , 2017, Scientific Reports.

[18]  Satyandra K. Gupta,et al.  Generating Simplified Trapping Probability Models From Simulation of Optical Tweezers System , 2009, J. Comput. Inf. Sci. Eng..

[19]  Marco Balucani,et al.  Development of Micro-Grippers for Tissue and Cell Manipulation with Direct Morphological Comparison , 2015, Micromachines.

[20]  Steven M Block,et al.  Direct observation of processive exoribonuclease motion using optical tweezers , 2015, Proceedings of the National Academy of Sciences.

[21]  A. Ashkin,et al.  History of optical trapping and manipulation of small-neutral particle, atoms, and molecules , 2000, IEEE Journal of Selected Topics in Quantum Electronics.

[22]  M. Sitti,et al.  Biohybrid Microtube Swimmers Driven by Single Captured Bacteria. , 2017, Small.

[23]  Won Gu Lee,et al.  Cell manipulation in microfluidics , 2013, Biofabrication.

[24]  Aaron Becker,et al.  Swarm control of cell-based microrobots using a single global magnetic field , 2013, 2013 10th International Conference on Ubiquitous Robots and Ambient Intelligence (URAI).

[25]  Jennifer E. Curtis,et al.  Dynamic holographic optical tweezers , 2002 .

[26]  Hongkai Wu,et al.  Recent Developments in Microfluidics for Cell Studies , 2014, Advanced materials.

[27]  Benjamin V. Johnson,et al.  Designing local magnetic fields and path planning for independent actuation of multiple mobile microrobots , 2017 .

[28]  Vijay Kumar,et al.  Automated biomanipulation of single cells using magnetic microrobots , 2013, Int. J. Robotics Res..

[29]  Xiang Li,et al.  Dynamic trapping and manipulation of biological cells with optical tweezers , 2013, Autom..

[30]  Ashis Gopal Banerjee,et al.  Toward automated formation of microsphere arrangements using multiplexed optical tweezers , 2016, NanoScience + Engineering.

[31]  Metin Sitti,et al.  Independent control of multiple magnetic microrobots in three dimensions , 2013, Int. J. Robotics Res..

[32]  Satyandra K. Gupta,et al.  Automated Manipulation of Biological Cells Using Gripper Formations Controlled By Optical Tweezers , 2014, IEEE Transactions on Automation Science and Engineering.

[33]  Satyandra K. Gupta,et al.  Optical Tweezers: Autonomous Robots for the Manipulation of Biological Cells , 2014, IEEE Robotics & Automation Magazine.

[34]  Nolan Wagener,et al.  Information theoretic MPC for model-based reinforcement learning , 2017, 2017 IEEE International Conference on Robotics and Automation (ICRA).

[35]  David J. Cappelleri,et al.  Towards Independent Control of Multiple Magnetic Mobile Microrobots† , 2015, Micromachines.

[36]  D B Phillips,et al.  A compact holographic optical tweezers instrument. , 2012, The Review of scientific instruments.

[37]  John Stewart,et al.  An accurate perception method for low contrast bright field microscopy in heterogeneous microenvironments , 2017 .

[38]  Satyandra K. Gupta,et al.  Indirect pushing based automated micromanipulation of biological cells using optical tweezers , 2014, Int. J. Robotics Res..

[39]  M. Jamal,et al.  Self-Folding Single Cell Grippers , 2014, Nano letters.

[40]  Giovanni Volpe,et al.  Computational toolbox for optical tweezers in geometrical optics , 2014, 1402.5439.

[41]  Daniel Isabey,et al.  Assessment of mechanical properties of adherent living cells by bead micromanipulation: comparison of magnetic twisting cytometry vs optical tweezers. , 2002, Journal of biomechanical engineering.