Electrochemical Characterization of Human Skin by Impedance Spectroscopy: The Effect of Penetration Enhancers

The electrochemical properties of human cadaver skin were studied in a diffusion cell with impedance spectroscopy as a function of time in the absence and presence of penetration enhancers dodecyl N,N-dimethylamino acetate and Azone. An improved electrochemical model of skin is presented, and combining the novel model with modern fractal mathematics, the effect of enhancers on the surface of skin is demonstrated. The enhancers appeared to open new penetration routes and increase the ohmic resistance, capacitive properties, and fractal dimension of skin, which means a rougher or more heterogeneous surface.

[1]  K. Kontturi,et al.  Transdermal iontophoresis of sotalol and salicylate; the effect of skin charge and penetration enhancers , 1993 .

[2]  J. Diard,et al.  Calculation, simulation and interpretation of electrochemical impedancesPart 3. Conditions for observation of low frequency inductive diagrams for a two-step electron transfer reaction with an adsorbed intermediate species , 1992 .

[3]  W. Higuchi,et al.  Skin alteration and convective solvent flow effects during iontophoresis: I. Neutral solute transport across human skin , 1991 .

[4]  B. Ongpipattanakul,et al.  Characterization of the permselective properties of excised human skin during iontophoresis. , 1987, Journal of pharmaceutical sciences.

[5]  T. Yamamoto,et al.  Electrical properties of the epidermal stratum corneum. , 1973, Medical & biological engineering.

[6]  C. Pepine,et al.  Rationale for use of glucocorticoids in modification of restenosis after percutaneous transluminal coronary angioplasty. , 1987, The American journal of cardiology.

[7]  J. Diard,et al.  Calculation, simulation and interpretation of electrochemical impedance: Part II. Interpretation of Volmer-Heyrovsky impedance diagrams , 1988 .

[8]  S. Grimnes,et al.  Skin impedance and electro-osmosis in the human epidermis , 1983, Medical and Biological Engineering and Computing.

[9]  B. Boukamp.  A Nonlinear Least Squares Fit procedure for analysis of immittance data of electrochemical systems , 1986 .

[10]  J. Denuzzio,et al.  Electrochemical and iontophoretic studies of human skin , 1990 .

[11]  M. Pikal,et al.  Transport Mechanisms in Iontophoresis. III. An Experimental Study of the Contributions of Electroosmotic Flow and Permeability Change in Transport of Low and High Molecular Weight Solutes , 1990, Pharmaceutical Research.

[12]  Peter M. Elias,et al.  Epidermal barrier function: intercellular lamellar lipid structures, origin, composition and metabolism , 1991 .

[13]  A. Ghanem,et al.  Transdermal iontophoretic drug delivery: mechanistic analysis and application to polypeptide delivery. , 1989, Journal of pharmaceutical sciences.

[14]  Michael J. Pikal,et al.  Transport Mechanisms in Iontophoresis. II. Electroosmotic Flow and Transference Number Measurements for Hairless Mouse Skin , 1990, Pharmaceutical Research.

[15]  Y. Yamamoto,et al.  The measurement principle for evaluating the performance of drugs and cosmetics by skin impedance , 1978, Medical and Biological Engineering and Computing.

[16]  S. Zucker,et al.  Evaluating the fractal dimension of surfaces , 1989, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.

[17]  S. M. Sims.  Iontophoretic transport mechanisms across skin , 1990 .

[18]  T. Pajkossy,et al.  Electrochemistry at fractal surfaces , 1991 .

[19]  Yoshitake Yamamoto,et al.  Analysis for the change of skin impedance , 1977, Medical and Biological Engineering and Computing.

[20]  JOHN W. Moore.  Membranes, ions, and impulses , 1976 .

[21]  A. van Boxtel,et al.  Skin resistance during square-wave electrical pulses of 1 to 10 mA , 1977, Medical and Biological Engineering and Computing.

[22]  J. Rosell,et al.  Skin impedance from 1 Hz to 1 MHz , 1988, IEEE Transactions on Biomedical Engineering.

[23]  B. Ongpipattanakul,et al.  Characterization of the pore transport properties and tissue alteration of excised human skin during iontophoresis. , 1988, Journal of pharmaceutical sciences.

[24]  Yie W. Chien,et al.  Iontophoretic delivery of drugs: Fundamentals, developments and biomedical applications , 1988 .

[25]  P. Paronen,et al.  Dodecyl N,N-Dimethylamino Acetate and Azone Enhance Drug Penetration Across Human, Snake, and Rabbit Skin , 1991, Pharmaceutical Research.

[26]  M. Pikal.  Transport Mechanisms in Iontophoresis. I. A Theoretical Model for the Effect of Electroosmotic Flow on Flux Enhancement in Transdermal Iontophoresis , 1990, Pharmaceutical Research.

[27]  T. Yamamoto,et al.  Dispersion and correlation of the parameters for skin impedance , 2006, Medical and Biological Engineering and Computing.