IL-17 siRNA promotes human proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK 1 / 2 , JNK and p 38 MAPK signaling

Objective: Inhibition of IL-17 on periodontal ligament stem cells (PDLSCs) proliferation osteogenic differentiation had been reported. This study was to investigate the effect of IL-17 on human PDLSCs (hPDLSCs). Methods: Isolated hPDLSCs from healthy fresh premolars were identified and then transfected with IL-17 silencing lentivirus plasmid. Then cell proliferation, colony formation, apoptosis, adipogenic and osteogenic differentiation was detected. Moreover, the activation of ERK1/2, JNK and p38 MAPK signaling was detected. Results: The isolated hPDLSCs were identified with > 99% percentage of STRO-1+/CD146+/CD44+/CD45cells. When transfected with IL-17 silencing lentivirus plasmid, about 50% percentage was STRO-1+/CD146+/CD44+/CD45+ cells. Moreover, cell proliferation, colony formation, osteogenic differentiation, as well as expression of p-ERK1/2, p-JNK and p-p38 MAPK were significantly enhanced. On contrast, adipogenic differentiations were inhibited by IL-17 silencing. No difference was observed in cell apoptotic percentage before and after transfection. Conclusion: We concluded that IL-17 siRNA administration promoted cell proliferation, colony formation, osteogenic differentiation of hPDLSCs via the activation of ERK1/2, JNK and p38 MAPK signaling.

[1]  C. Mao,et al.  Double-edged-sword effect of IL-1β on the osteogenesis of periodontal ligament stem cells via crosstalk between the NF-κB, MAPK and BMP/Smad signaling pathways , 2016, Cell Death and Disease.

[2]  J. Krstić,et al.  The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs. , 2016, The international journal of biochemistry & cell biology.

[3]  E. Mazzon,et al.  Alternative source of stem cells derived from human periodontal ligament: a new treatment for experimental autoimmune encephalomyelitis , 2016, Stem Cell Research & Therapy.

[4]  S. Miscia,et al.  Human Periodontal Stem Cells Release Specialized Proresolving Mediators and Carry Immunomodulatory and Prohealing Properties Regulated by Lipoxins , 2015, Stem cells translational medicine.

[5]  Gao-qin Liu,et al.  The effects of interleukin 17 on the proliferation, migration and apoptosis of human retinal vascular endothelial cells , 2015 .

[6]  A. Magnan,et al.  IL-17 in severe asthma. Where do we stand? , 2014, American journal of respiratory and critical care medicine.

[7]  Youngkyun Lee,et al.  IL-17 inhibits osteoblast differentiation and bone regeneration in rat. , 2014, Archives of oral biology.

[8]  Q. Shi,et al.  Bone Morphogenetic Protein-9 Induces PDLSCs Osteogenic Differentiation through the ERK and p38 Signal Pathways , 2014, International journal of medical sciences.

[9]  L. Cui,et al.  Salvianolic acid B promotes osteogenesis of human mesenchymal stem cells through activating ERK signaling pathway. , 2014, The international journal of biochemistry & cell biology.

[10]  P. Pavasant,et al.  High Glucose Condition Suppresses Neurosphere Formation by Human Periodontal Ligament‐Derived Mesenchymal Stem Cells , 2014, Journal of cellular biochemistry.

[11]  Jinhua Yu,et al.  Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways , 2014, BioMed research international.

[12]  Dajin Li,et al.  Decidual stromal cells recruit Th17 cells into decidua to promote proliferation and invasion of human trophoblast cells by secreting IL-17 , 2014, Cellular and Molecular Immunology.

[13]  Malcolm L Snead,et al.  Application of stem cells derived from the periodontal ligament or gingival tissue sources for tendon tissue regeneration. , 2014, Biomaterials.

[14]  Zhihe Zhao,et al.  The osteogenic differentiation of PDLSCs is mediated through MEK/ERK and p38 MAPK signalling under hypoxia. , 2013, Archives of oral biology.

[15]  Yin Ding,et al.  Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments. , 2013, International journal of molecular medicine.

[16]  S. Gaffen,et al.  IL-17 inhibits adipogenesis in part via C/EBPα, PPARγ and Krüppel-like factors. , 2013, Cytokine.

[17]  Y. Ding,et al.  Nuclear factor-κB modulates osteogenesis of periodontal ligament stem cells through competition with β-catenin signaling in inflammatory microenvironments , 2013, Cell Death and Disease.

[18]  G. Armitage.  Bi-directional relationship between pregnancy and periodontal disease. , 2013, Periodontology 2000.

[19]  W. Zou,et al.  IL‐17 mediates estrogen‐deficient osteoporosis in an Act1‐dependent manner , 2012, Journal of cellular biochemistry.

[20]  Hongwei Liu,et al.  A novel mixed-type stem cell pellet for cementum/periodontal ligament-like complex. , 2012, Journal of periodontology.

[21]  Ming Yan,et al.  Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways , 2012, Histochemistry and Cell Biology.

[22]  Seong-Ho Choi,et al.  Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations. , 2011, Journal of clinical periodontology.

[23]  Wei Wang,et al.  Allogeneic Periodontal Ligament Stem Cell Therapy for Periodontitis in Swine , 2010, Stem cells.

[24]  H. J. Kim,et al.  IL-17 stimulates the proliferation and differentiation of human mesenchymal stem cells: implications for bone remodeling , 2009, Cell Death and Differentiation.

[25]  S. Gaffen,et al.  A bone‐protective role for IL‐17 receptor signaling in ovariectomy‐induced bone loss , 2009, European journal of immunology.

[26]  Y. Miao,et al.  Stimulation of osteogenic differentiation and inhibition of adipogenic differentiation in bone marrow stromal cells by alendronate via ERK and JNK activation. , 2008, Bone.

[27]  Ki Dong Park,et al.  ERK 1/2 activation in enhanced osteogenesis of human mesenchymal stem cells in poly(lactic-glycolic acid) by cyclic hydrostatic pressure. , 2007, Journal of biomedical materials research. Part A.

[28]  L. V. Van Gaal,et al.  Human leptin: from an adipocyte hormone to an endocrine mediator. , 2000, European journal of endocrinology.

[29]  H. Löe.  Periodontal Disease: The sixth complication of diabetes mellitus , 1993, Diabetes Care.

[30]  P. Papapanou,et al.  Epidemiology of association between maternal periodontal disease and adverse pregnancy outcomes--systematic review. , 2013, Journal of clinical periodontology.

[31]  P. Buekens,et al.  Periodontal disease as a potential risk factor for the development of diabetes in women with a prior history of gestational diabetes mellitus. , 2013, Journal of public health dentistry.