Murine B cell differentiation lineages

Subpopulations of mouse B cells express different amounts of two antigens (BLA-1 and BLA-2) recognized by rat monoclonal antibodies (53- 10.1 and 30-E2). Two-color immunofluorescence analysis on the fluorescence-activated cell sorter (FACS) shows that the 53-10.1 monoclonal antibody reacts with a similar proportion of splenic B cells from normal and CBA/N (xid) mice, whereas 30-E2 reacts with most CBA/N B cells but with only a fraction of normal B cells. Data from three- and four-color immunofluorescence analyses with xid, athymic (nude), and normal mice suggest that the order in which these antigens are lost during B cell differentiation distinguishes two B cell lineages: immature B cells express both antigens, intermediate-stage B cells of one or the other lineage express only BLA-1 or only BLA-2, respectively, and mature resting B cells express neither. CBA/N mice lack one of the putative intermediate populations (BLA-1+,2-); thus, this population apparently gives rise to the predominant mature B cell population, which is present in normal adult spleen and lymph node but is missing in CBA/N. The other putative intermediate population (BLA-1- ,2+) is decreased by two- to threefold in spleens from nude mice compared with strain-matched controls. Both BLA-1 and BLA-2 antigens rapidly reappear after specific (antigen) or nonspecific (lipopolysaccharide) B cell activation. IgM plaque-forming cells (PFC) derived from such activated cells continue to express both antigens while IgG PFC express only BLA-1.

[1]  M. Wilchek,et al.  The use of the avidin-biotin complex as a tool in molecular biology. , 2006, Methods of biochemical analysis.

[2]  R. Hardy,et al.  Three-color immunofluorescence analysis of mouse B-lymphocyte subpopulations. , 1984, Cytometry.

[3]  R. Hardy,et al.  Demonstration of B-cell maturation in X-linked immunodeficient mice by simultaneous three-colour immunofluorescence , 1983, Nature.

[4]  R. Hardy,et al.  Dual immunofluorescence - new frontiers in cell analysis and sorting. , 1983, Immunology today.

[5]  R. Hardy,et al.  The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice , 1983, The Journal of experimental medicine.

[6]  W. Paul,et al.  Analysis of B Lymphocyte Differentiation Antigens by Flow Microfluorometry , 1983, Immunological reviews.

[7]  R. Hardy,et al.  B‐CELL SUBPOPULATIONS IDENTIFIABLE BY TWO‐COLOR FLUORESCENCE ANALYSIS USING A DUAL‐LASER FACS * , 1982, Annals of the New York Academy of Sciences.

[8]  R. Hardy,et al.  Molecular, Cellular and Systemic Mechanisms for Regulating IgCH Expression , 1982, Immunological reviews.

[9]  I. Weissman,et al.  Germinal centre B cells: antigen specificity and changes in heavy chain class expression , 1982, Nature.

[10]  R. Hardy,et al.  B-cell subpopulations identified by two-colour fluorescence analysis , 1982, Nature.

[11]  Aftab J. Ahmed,et al.  Distinct B Cell Subpopulations Differ in Their Genetic Requirements for Activation by T Helper Cells , 1982, Immunological reviews.

[12]  L. Stryer,et al.  Fluorescent phycobiliprotein conjugates for analyses of cells and molecules , 1982, The Journal of cell biology.

[13]  W. Paul,et al.  Regulation of Growth and Proliferation in B Cell Subpopulations , 1982, Immunological reviews.

[14]  L. Burkly,et al.  Lack of mature B cells in nude mice with X-linked immune deficiency , 1982, The Journal of experimental medicine.

[15]  W. Paul,et al.  Role of the thymus in directing the development of a subset of B lymphocytes , 1982, The Journal of experimental medicine.

[16]  L. Lanier,et al.  Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. , 1981, Journal of immunology.

[17]  W. Faulk,et al.  Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies. , 1981, Journal of immunological methods.

[18]  P. Kincade,et al.  Monoclonal rat antibodies to murine IgM determinants. , 1981, Journal of immunological methods.

[19]  V. Stevens,et al.  A method for preparing beta-hCG COOH peptide-carrier conjugates of predictable composition. , 1980, Molecular immunology.

[20]  L. Herzenberg,et al.  Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens * , 1979, Immunological reviews.

[21]  Ahmed Awad E. Ahmed,et al.  Lyb-7, a new B cell alloantigen controlled by genes linked to the IgCH locus. , 1979, Journal of immunology.

[22]  J Carlsson,et al.  Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. , 1978, The Biochemical journal.

[23]  Y. Li,et al.  Preparation of protein conjugates via intermolecular disulfide bond formation. , 1978, Biochemistry.

[24]  Ahmed Awad E. Ahmed,et al.  Surface Immunoglobulin D as a Functional Receptor for a Subclass of B Lymphocytes , 1977, Immunological reviews.

[25]  D R Parks,et al.  Two-color immunofluorescence using a fluorescence-activated cell sorter. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[26]  P. Kincade.  Defective colony formation by B lymphocytes from CBA/N and C3H/HeJ mice , 1977, The Journal of experimental medicine.

[27]  H. Cantor,et al.  Identification of a B-cell surface structure involved in antigen- dependent triggering: absence of this structure on B cells from CBA/N mutant mice , 1977, The Journal of experimental medicine.

[28]  J. Goding.  Conjugation of antibodies with fluorochromes: modifications to the standard methods. , 1976, Journal of immunological methods.

[29]  M. Rittenberg,et al.  Evidence for separate subpopulations of B cells responding to T-independent and T-dependent immunogens. , 1976, Journal of immunology.

[30]  W. Paul,et al.  X-linked B-lymphocyte defect in CBA/N mice. III. Abnormal development of B-lymphocyte populations defined by their density of surface immunoglobulin , 1976, The Journal of experimental medicine.

[31]  M. Raff,et al.  Early production of intracellular IgM by B-lymphocyte precursors in mouse , 1976, Nature.

[32]  W. Paul,et al.  X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanisms underlying the immune defect , 1975, The Journal of experimental medicine.

[33]  J. Playfair,et al.  Separate thymus dependent and thymus independent antibody forming cell precursors. , 1971, Nature: New biology.

[34]  A. Cunningham,et al.  Further improvements in the plaque technique for detecting single antibody-forming cells. , 1968, Immunology.

[35]  A. Glazer,et al.  Phycobilisomes: structure and dynamics. , 1982, Annual review of microbiology.

[36]  L. Herzenberg,et al.  Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. , 1978, Current topics in microbiology and immunology.