Suppressive effects of low-power laser irradiation on bradykinin evoked action potentials in cultured murine dorsal root ganglion cells

The effects of Ga-Al-As diode laser (830 nm, 16.2 mW) irradiation on the distal portion of the processes of cultured murine dorsal root ganglion (DRG) neurons associated with C-fibers were studied by patch-clamp whole-cell recording of membrane potentials at the cell body. The chemical as well as laser light stimulations were limited to the processes of the neuron isolated from the cell body with a separator. The action potentials elicited by bradykinin (BK) in the cell body were reversibly suppressed by the irradiation of laser light. The laser irradiation may block the conduction of nociceptive signals in primary afferent neurons. The present experimental method offers a simple and easy to use procedure for studying the pain relief effects by laser irradiation.

[1]  L. Snyder-Mackler,et al.  Effect of helium-neon laser irradiation on peripheral sensory nerve latency. , 1988, Physical therapy.

[2]  L. Mackler,et al.  The effect of helium neon laser on latency of sensory nerve , 1985 .

[3]  M. Kawatani,et al.  Diode laser irradiation selectively diminishes slow component of axonal volleys to dorsal roots from the saphenous nerve in the rat , 1993, Neuroscience Letters.

[4]  T. Kreczi,et al.  A comparison of laser acupuncture versus placebo in radicular and pseudoradicular pain syndromes as recorded by subjective responses of patients. , 1986, Acupuncture & electro-therapeutics research.

[5]  土屋 喜由.  Laser irradiation abates neuronal responses to nociceptive stimulation of rat-paw skin , 1995 .

[6]  Fmv. Plog.  Biophysical application of the laser beam , 1980 .

[7]  M. Yanase,et al.  Therapeutic effect of Ga‐Al‐As diode laser irradiation on experimentally induced inflammation in rats , 1992, Lasers in surgery and medicine.

[8]  A. Cameron,et al.  The electrophysiological and morphological characteristics of feline dorsal root ganglion cells , 1986, Brain Research.

[9]  T. Maeda.  MORPHOLOGICAL DEMONSTRATION OF LOW REACTIVE LASER THERAPEUTIC PAIN ATTENUATION EFFECT OF THE GALLIUM ALUMINIUM ARSENIDE DIODE LASER , 1989 .

[10]  P. Hogan,et al.  Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Pekka J. Pöntinen,et al.  Low level laser therapy as a medical treatment modality : a manual for physicians, dentists, physiotherapists and veterinary surgeons , 1992 .

[12]  Effects of Diode Laser Irradiation on Sensory Ganglion Cells from the Rat , 1996 .

[13]  A. Welch,et al.  Laser‐irradiation‐induced relaxation of blood vessels in vivo , 1990, Lasers in surgery and medicine.

[14]  K. Yoda,et al.  Excitatory effects of algesic compounds on neuronal processes in murine dorsal root ganglion cell culture , 1997, Brain Research.

[15]  J. Wood,et al.  Bradykinin evoked depolarization of a novel neuroblastoma × DRG neurone hybrid cell line (ND723) , 1991, Brain Research.