Natural occurrence of Aflatoxin B1 in peanut collected from Kinshasa, Democratic Republic of Congo

Aflatoxin B1 is a potent carcinogen to both animal and human health. Since peanut is a suitable substrate for aflatoxin production as well as an important oilseed and food in the Democratic Republic of Congo, the risk of consuming aflatoxin-contaminated peanuts is very high. This paper assessed the natural occurrence of aflatoxin B1 in raw peanuts collected in rural areas of Kinshasa, Democratic Republic of Congo. A total of 60 peanut samples were analyzed for aflatoxin B1, using thin layer chromatography. The results show that aflatoxin B1 levels increased from the dry season to the rainy season with values ranging from 1.5 to 390 and 12 to 937, respectively. 70% of the peanut samples from both seasons exceeded the maximum limit of 5 μg/kg prescribed by the World Health Organization (WHO). As the Democratic Republic of Congo is amongst African countries listed with high prevalence of liver cancer, continuous research on aflatoxin B1 is sought after.

[1]  M. Moss.  Risk assessment for aflatoxins in foodstuffs , 2002 .

[2]  R. Kendall,et al.  Comparative acute and combinative toxicity of aflatoxin B1 and T‐2 toxin in animals and immortalized human cell lines , 2006, Journal of applied toxicology : JAT.

[3]  I. Purchase,et al.  Hepatocellular carcinoma and dietary aflatoxin in Mozambique and Transkei. , 1985, British Journal of Cancer.

[4]  C. Wannop.  Turkey "X" disease. , 1961 .

[5]  S. Kazadi,et al.  Interannual and long‐term climate variability over the Zaire River Basin during the last 30 years , 1996 .

[6]  F. X. Bosch,et al.  Reducing Liver Cancer--Global Control of Aflatoxin , 1999, Science.

[7]  H. Whittle,et al.  Hepatitis B infection and aflatoxin biomarker levels in Gambian children , 2000, Tropical medicine & international health : TM & IH.

[8]  M. Clements,et al.  Mortality in the Democratic Republic of Congo: a nationwide survey , 2006, The Lancet.

[9]  S. Macfarlane,et al.  Aflatoxins and kwashiorkor. Epidemiology and clinical studies in Sudanese children and findings in autopsy liver samples from Nigeria and South Africa. , 1983, Bulletin de la Societe de pathologie exotique et de ses filiales.

[10]  V. Freitas,et al.  Occurrence of aflatoxins B1, B2, G1, and G2 in peanuts and their products marketed in the region of Campinas, Brazil in 1995 and 1996. , 1998, Food additives and contaminants.

[11]  R B Jones,et al.  Prevalence and factors associated with aflatoxin contamination of peanuts from Western Kenya. , 2009, International journal of food microbiology.

[12]  R. Clark,et al.  Health Effects of Mycotoxins: A Toxicological Overview , 2004, Journal of toxicology. Clinical toxicology.

[13]  R. R. Marquardt.  Effects of molds and their toxins on livestock performance: a western Canadian perspective , 1996 .

[14]  D. Eaton,et al.  5 – Nonhepatic Disposition and Effects of Aflatoxin B1 , 1993 .

[15]  O. Kawamura,et al.  Aflatoxins and fumonisins in corn from the high-incidence area for human hepatocellular carcinoma in Guangxi, China. , 2001, Journal of agricultural and food chemistry.

[16]  S. A. Bankole,et al.  Mycotoxins in food in West Africa: current situation and possibilities of controlling it , 2003 .

[17]  P. Hamilton,et al.  Dietary exposure of broiler breeders to aflatoxin results in immune dysfunction in progeny chicks. , 1998, Poultry science.

[18]  John Davis Groopman,et al.  The toxicology of aflatoxins: human health, veterinary and agricultural significance. , 1993 .

[19]  A J Hall,et al.  Dietary aflatoxin exposure and impaired growth in young children from Benin and Togo: cross sectional study , 2002, BMJ : British Medical Journal.

[20]  J. Miller,et al.  Mycotoxins in grain: compounds other than aflatoxin. , 1994 .

[21]  J. P. F. D'Mello,et al.  Food Safety: Contaminants and Toxins , 2003 .

[22]  A. Ramos,et al.  In vitro aflatoxin adsorption by means of a montmorillonite silicate. A study of adsorption isotherms , 1996 .

[23]  G. Shephard.  Aflatoxin and Food Safety: Recent African Perspectives , 2003 .

[24]  J. Wagacha,et al.  Mycotoxin problem in Africa: current status, implications to food safety and health and possible management strategies. , 2008, International journal of food microbiology.

[25]  J. Bennett,et al.  Mycotoxins and Mycotoxicoses , 1980 .

[26]  J Stroka,et al.  Immunoaffinity column clean-up prior to thin-layer chromatography for the determination of aflatoxins in various food matrices. , 2000, Journal of chromatography. A.

[27]  R. Kendall,et al.  Comparative acute and combinative toxicity of aflatoxin B1 and fumonisin B1 in animals and human cells. , 2006, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[28]  D. Mcdonald,et al.  Growth of Aspergillus flavus and production of aflatoxin in groundnuts. Part VIII. , 1963 .

[29]  Henry Njapau,et al.  Aflatoxin Contamination of Commercial Maize Products during an Outbreak of Acute Aflatoxicosis in Eastern and Central Kenya , 2005, Environmental health perspectives.

[30]  D. Arora.  Fungal Biotechnology in Agricultural, Food, and Environmental Applications , 2003 .

[31]  H. S. Hussein,et al.  Toxicity, metabolism, and impact of mycotoxins on humans and animals. , 2001, Toxicology.

[32]  E. Papp,et al.  Liquid chromatographic determination of aflatoxins , 2002 .

[33]  T. Otsuki,et al.  What price precaution? European harmonisation of aflatoxin regulations and African groundnut exports , 2001 .

[34]  D M Parkin,et al.  Estimates of the worldwide frequency of sixteen major cancers in 1980 , 1988, International journal of cancer.

[35]  M. Moss.  Microbial food poisoning , 1987 .

[36]  A. Prentice,et al.  Modification of immune function through exposure to dietary aflatoxin in Gambian children. , 2003, Environmental health perspectives.

[37]  K. Cardwell,et al.  The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, west Africa. , 2000, Journal of stored products research.

[38]  E. Creppy.  Update of survey, regulation and toxic effects of mycotoxins in Europe. , 2002, Toxicology letters.

[39]  H. Njapau,et al.  The effect of village processing techniques on the content of aflatoxins in corn and peanuts in Zambia , 1998 .

[40]  W. Horwitz.  Official Methods of Analysis , 1980 .

[41]  H. P. Egmond.  Current situation on regulations for mycotoxins. Overview of tolerances and status of standard methods of sampling and analysis. , 1989 .

[42]  R. Baht,et al.  Occurrence of aflatoxin and its economic impact on human nutrition and animal feed : the new regulations , 1999 .

[43]  L. Rocha,et al.  DISTRIBUTION OF FUNGI AND AFLATOXINS IN A STORED PEANUT VARIETY , 2008 .

[44]  J. D'mello.  Mycotoxins in cereal grains, nuts and other plant products. , 2003 .